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Plasmid#119770PurposeConversion of C to T in wheat plants or monocotyledons protoplasts without uracil glycosylase inhibitor (UGI)DepositorInsertAPOBEC3A-nCas9-ΔUGI-NLS
UseTagsNLSExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9Promotermaize Ubiquitin-1Available sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 STAT3
Plasmid#67120PurposeStandard for cell-free expression benchmarking.DepositorInsertSTAT3 (STAT3 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c-rac2 guide
Plasmid#107600PurposeAn entry vector with U6a and U6c promoter driving rac2 guide RNAs expressionDepositorInsertrac2 guide RNA sequences
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-TYK2V362F-VSV
Plasmid#139345PurposeExpression of TYK2 V362F protein (rs2304256)DepositorInsertTYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
UseTagsVSVExpressionMammalianMutationV362FPromoterCMVAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMEH305 HA NLS SpCas9 3x high affinity MS2 loops
Plasmid#168216PurposeMammalian expression SV40 and nucleoplasmin NLS SpCas9 with 3 high affinity MS2 loops and fused to an N-terminal HA tagDepositorInsertSV40 NLS SpCas9
UseLentiviralTagsHA, SV40 NLS, and nucleoplasmin NLSExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-p27(kip1)
Plasmid#50026PurposepENTR vector for human cyclin-dependent kinase inhibitor 1BDepositorInsertp27(kip1) (CDKN1B Human)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceDec. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shPrc1
Plasmid#160959PurposePrc1 shRNA in pMKO.1 retroviral vectorDepositorInsertPrc1 shRNA (Prc1 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TET1-848-952(pL91)
Plasmid#124388PurposeE.Coli produced TET1-848-952 purified by FPLC nickel columnDepositorInsertGB1-His-TET1-848-952 wildtype (TET1 Human)
UseTagsGB1-HistidineExpressionBacterialMutationPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-SAP25-126-186(pL91)
Plasmid#124387PurposeE.Coli produced SAP25 SID purified by FPLC nickel columnDepositorInsertGB1-His-SAP25-126-186 (SAP25 Human)
UseTagsGB1-HistidineExpressionBacterialMutationPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-BE3-2NLS
Plasmid#119031PurposeStably express BE3 in mammalian cellsDepositorInsertBE3
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-shMdk-334
Plasmid#99025PurposeExpresses shRNA targeting mouse Mdk (midkine)DepositorInsertMdk shRNA (Mdk Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromotermouse U6Available sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
YFP(1-158) in pcDNAI/Amp
Plasmid#60888PurposeThis vector attaches YFP(1-158) to the N-terminus of a protein.DepositorInsertYFP(1-158)
UseTagsExpressionMammalianMutationMet was substituted for Gln-69.PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
rGSKmyc-R85-pXT7
Plasmid#15493DepositorInsertGSK-3 beta R85 (Gsk3b Rat)
UseXenopus rna expressionTagsmycExpressionMutationR85: K85R, dominant negativePromoterAvailable sinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TET1-878-911(pL91)
Plasmid#124390PurposeE.Coli produced TET1 SID (TET1-878-911) purified by FPLC nickel columnDepositorInsertGB1-His-TET1-878-911 wildtype (TET1 Human)
UseTagsGB1-HistidineExpressionBacterialMutationPromoterAvailable sinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
AV4-EF1-GFP
Plasmid#124971PurposeControl for AV4-EF1-NMNAT2DepositorInsertGFP
UseAAVTagsExpressionMutationPromoterEF1-alphaAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAL950
Plasmid#119748Purposeprecursor outer membrane pore protein E, phoE with mutations Alanine 249 to Cysteine and Glutamine 307 to CysteineDepositorInsertprecusor Outer membrane pore protein E, phoE, with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine
UseTagsnoneExpressionBacterialMutationInternal NdeI site removed and mutations Alanine …PromoterT7Available sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-BE3-3NLS
Plasmid#119032PurposeStably express BE3 in mammalian cellsDepositorInsertBE3
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
YFP(159-238) in pcDNAI/Amp
Plasmid#60889PurposeThis vector attaches YFP(159-238) to the N-terminus of a protein.DepositorInsertYFP(159-238)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST39-Hrs-STAM1 core
Plasmid#21497DepositorUseTagsHisExpressionBacterialMutationnonePromoterAvailable sinceFeb. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_STAM-1/1
Plasmid#91428PurposeProtein expression and purification of human SH3 domain construct STAM-1/1DepositorInsertSTAM-1/1 (STAM Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only