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Plasmid#139462PurposeLentiviral vector with gRNA scaffold and hygromycin selectable markerDepositorInsertno sgRNA inserted; resistance gene: hygroR
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCas
Plasmid#62225PurposeConstitutive expression of cas9 and inducible expression of lambda RED and sgRDepositorHas ServiceCloning Grade DNAInsertcas9
UseCRISPR; Lambda red recombinaseTagsExpressionBacterialMutationPromoternative cas9 promoterAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-EGFP (AAV Retrograde trial size)
Viral Prep#50465-AAVrg.TPurposeReady-to-use AAV Retrograde trial size particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons.DepositorPromoterSynTagsEGFPAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDY0663 ACTB atgRNA with v2 scaffold
Plasmid#179108PurposeACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA with v2 scaffoldDepositorInsertACTB N-term PBS 13 RT 29 AttB 46 atgRNA with v2 scaffold
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ma-pREP
Plasmid#197571PurposePositive selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses TAG-codon interrupted chloramphenicol resistance cassette and M. alvus Pyl-tRNA(6). p15a oriDepositorInsertsChloramphenicol resistance gene - 111TAG
T7 polymerase -TAG
GFPuv
M. alvus Pyl-tRNA(6)
UseTagsExpressionBacterialMutation111TAG and 8TAG/112TAGPromoterAraC, T7, cat promoter (constitutive), and lpp (c…Available sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1346 U6-B2M sgRNA Gag-pol v2
Plasmid#201917PurposeCas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)DepositorInsertsGag-pol
B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
UseTagsExpressionMammalianMutationPromoterCAG and Human U6Available sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-CamKIIa-jGCaMP8m-WPRE (AAV1)
Viral Prep#176751-AAV1PurposeReady-to-use AAV1 particles produced from AAV-CamKIIa-jGCaMP8m-WPRE (#176751). In addition to the viral particles, you will also receive purified AAV-CamKIIa-jGCaMP8m-WPRE plasmid DNA. CamKIIa-driven expression of calcium sensor GCaMP8m. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCamKIIalphaTagsNoneAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pALS2-sfGFP 150TAG
Plasmid#197574PurposeFluorescence selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-150TAG and M. alvus Pyl-tRNA(6). p15a origin of replication.DepositorInsertssfGFP 150TAG
M. alvus Pyl-tRNA (6)
UseTagsHis6ExpressionBacterialMutationN150TAGPromoteraraC and lppAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLL7.0: tgRFPt-SSPB WT
Plasmid#60415PurposeMammalian Expression of tgRFPt-NanoDepositorInserttgRFPt-SSPB
UseCre/Lox and LentiviralTagsTagRFPtExpressionMammalianMutationY11K and A15EPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28:GFP
Plasmid#60733PurposepET28 driving expression of GFP, biobrick part Bba_E0040DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-tdTomato (AAV2)
Viral Prep#28306-AAV2PurposeReady-to-use AAV2 particles produced from pAAV-FLEX-tdTomato (#28306). In addition to the viral particles, you will also receive purified pAAV-FLEX-tdTomato plasmid DNA. CAG-driven, Cre-dependent tdTomato expression control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagstdTomato (Cre-dependent)Available sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-dLight1.3b (AAV9)
Viral Prep#135762-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-syn-dLight1.3b (#135762). In addition to the viral particles, you will also receive purified pAAV-syn-dLight1.3b plasmid DNA. Syn-driven expression of dLight1.3b dopamine sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLCK-mScarlet3_C1
Plasmid#189771PurposeConstruct for labelling plasma membranes in mammalian cells using LCK-mScarlet3DepositorInsertLCK-mScarlet3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmU6-gRNA
Plasmid#53187PurposeExpresses the S. pyogenes sgRNA from the mouse U6 promoterDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromotermouse U6Available sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-GfaABC1D-MCS--4x6T-WPRE
Plasmid#196417PurposeEmpty vector for astrocyte-selective expression in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassetteDepositorTypeEmpty backboneUseAAV; Astrocyte-selectiveTagsExpressionMammalianMutationPromoterGfaABC1DAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLEX_305-C-dTAG
Plasmid#91798PurposeLentiviral/gateway cloning vector for C-terminally tagging proteins of interest with the dTAG systemDepositorTypeEmpty backboneUseLentiviralTagsFKBP F36VExpressionMammalianMutationPromoterhPGKAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV MCS mCherry-KASH
Plasmid#139654PurposeCloning template for AAV-based ORANGE knock-in constructsDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterU6Available sinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-HNF4A-FOXA2-FOXA3
Plasmid#149724Purposeexpressing three liver promoting transcription factors (HNF4A, FOXA2, FOXA3)DepositorInsertHNF4A-FOXA2-FOXA3
UseLentiviralTagsP2A, T2AExpressionMutationPromoterCMVAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEN114 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-PuroR-bpA-Frt-Rosa26
Plasmid#92143PurposeTargeting vector to introduce an osTir1 expressing cassette at the mouse Rosa26 locus using Puromycin selection. Auxin-inducible degron system.DepositorInsertosTir1
UseMouse TargetingTagsV5 tagExpressionMammalianMutationPromoterAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-VSVN
Plasmid#64087PurposeExpression vector for Vesicular Stomatitis Virus Indiana strain nucleoprotein geneDepositorInsertVSV nucleoprotein
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only