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p221- purVSG117UTR

Plasmid

#59732

Purpose

Inserts a puromycin resistance gene and a VSG117 gene into the VSG221 expression site of T.brucei downstream of the expression site promoter.

Depositor

Gloria Rudenko

Article

Smith et al PLoS One. 2009 Oct 26;4(10):e7532. doi:

Insert

VSG117 flanked upstream and downstream by sequences homologous to the VSG221 expression site

Use

For homologous recombination into vsg221 expressi…

Tags

Expression

Mutation

Promoter

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-hGRM1-RFP

Plasmid

#22920

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 . 3():19.

Insert

human receptor metabotropic 1 promoter driving DsRedExpress (GRM1 Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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HOXB4 (human) FLAG MSCV

Plasmid

#8528

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB4 (HOXB4 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Missing N-terminal 14 amino acids; improved Ribos…

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pAAV-mA93-RFP

Plasmid

#22918

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 . 3():19.

Insert

mouse riken gene A930038C07Rik promoter driving DsRedExpress (A930038C07Rik Mouse)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2010

Availability

Academic Institutions and Nonprofits only

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HOXB4 (human) HA-TAT-tag pET

Plasmid

#8526

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB4 (HOXB4 Human)

Use

Tags

HA

Expression

Bacterial

Mutation

replace T7 and HIS tags of pET with an HA tag;

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pLenti-sgCCR5

Plasmid

#83930

Purpose

Lentiviral vector expressing an sgRNA targeting CCR5 NOTE: This plasmid does NOT contain Cas9.

Depositor

David Sabatini

Article

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

sgCCR5

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-HDAC4 C-terminus

Plasmid

#71400

Purpose

CAG promoter, mammalian expression

Depositor

Bo Chen

Article

Guo et al Nat Commun. 2015 Aug 14;6:8005. doi: 10.

Insert

HDAC4 C-terminus (HDAC4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 29, 2015

Availability

Academic Institutions and Nonprofits only
pFLAG-4S Tetherin

Plasmid

#41073

Depositor

Paul Spearman

Article

Hammonds et al J Virol. 2012 Feb;86(4):2259-72. Epub 20

Insert

Tetherin (BST2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S74, S84, 137S, and 144S

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pEMS2049

Plasmid

#79657

Purpose

ssAAV genome with Ple260 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

ssAAV Ple260-EmGFP WPRE

Use

AAV

Tags

Expression

Mutation

Promoter

PAX6 Retinal Enhancer

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pEMS2045

Plasmid

#79653

Purpose

ssAAV genome with Ple256 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

ssAAV Ple256-EmGFP WPRE

Use

AAV

Tags

Expression

Mutation

Promoter

PAX6 enhancer

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pFLAG-137.144S Tetherin

Plasmid

#41072

Depositor

Paul Spearman

Article

Hammonds et al J Virol. 2012 Feb;86(4):2259-72. Epub 20

Insert

Tetherin (BST2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

137S, 144S

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pEMS2048

Plasmid

#79656

Purpose

ssAAV genome with Ple259 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

ssAAV Ple259-EmGFP WPRE

Use

AAV

Tags

Expression

Mutation

Promoter

PAX6 MiniPromoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-HDAC4 1-747

Plasmid

#71399

Purpose

CAG promoter, mammalian expression

Depositor

Bo Chen

Article

Guo et al Nat Commun. 2015 Aug 14;6:8005. doi: 10.

Insert

HDAC4 1-747 (HDAC4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pEMS2043

Plasmid

#79651

Purpose

ssAAV genome with Ple254 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

ssAAV Ple254-EmGFP WPRE

Use

AAV

Tags

Expression

Mutation

Promoter

PAX6 Retinal Enhancer

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hADM-RFP

Plasmid

#22922

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 . 3():19.

Insert

human adrenomedullin promoter driving DsRedExpress (ADM Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pFLAG-74.84S Tetherin

Plasmid

#41071

Depositor

Paul Spearman

Article

Hammonds et al J Virol. 2012 Feb;86(4):2259-72. Epub 20

Insert

Tetherin (BST2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

74S, 84S

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pCAG-HDAC4 1-129

Plasmid

#71401

Purpose

CAG promoter, mammalian expression

Depositor

Bo Chen

Article

Guo et al Nat Commun. 2015 Aug 14;6:8005. doi: 10.

Insert

HDAC4 1-129 (HDAC4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
10XUAS IVS Jaws mCherry_tr

Plasmid

#111550

Purpose

Optogenetics in Drosophila: UAS-induced expression of Jaws mCherry

Depositor

Vivek Jayaraman

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws_mCherry_trafficked

Use

Tags

Expression

Insect

Mutation

wt

Promoter

hsp70

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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20XUAS IVS Jaws mVenus_tr

Plasmid

#111552

Purpose

Optogenetics in Drosophila: UAS-induced expression of Jaws mVenus

Depositor

Vivek Jayaraman

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws_mVenus_tr

Use

Tags

Expression

Insect

Mutation

wt

Promoter

hsp70

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pEMS1932

Plasmid

#79781

Purpose

High copy number pUC vector containing Ple259 (PAX6 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

Ple259

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Expression

Mutation

Promoter

Ple259 (PAX6 MiniPromoter)

Available since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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