Purpose
Egg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPC
Inserts
sgRNA targeting TRY and CPC genes
sgRNA targeting ETC2 gene
zCas9
Use
CRISPR; Plant binary vector
Tags
3x FLAG and NLS
Expression
Plant
Mutation
Zea mays codon-optimized Cas9
Promoter
EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
Available since
Jan. 14, 2016
Availability
Academic Institutions and Nonprofits only