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Plasmid#141417PurposeExpress scFvGCN4-sfGFP-VP64 fusion protein for dCas9-SunTag mediated targeted gene activationDepositorInsertscFvGCN4sfGFP-VP64-GB1
UseTagsHA and sfGFPExpressionMammalianMutationPromoterCAGAvailable sinceJune 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFBD-SpyTag-noro-VLP
Plasmid#165989PurposeExpresses norovirus-like particles with SpyTags presented on their surface for convenient decoration of SpyCatcher fusion proteinsDepositorInsertSpyTag-noro-VLP (ORF2 norovirus, Synthetic)
UseTagsSpyTagExpressionInsectMutationPromoterPolyhedrinAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-SV40-Galpha-i3 ONE-GO
Plasmid#204329PurposeGPCR/G protein BRET biosensorDepositorInsertSV40 Galpha-i3 ONE-GO
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P28
Plasmid#120039PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xHADepositorInsert3xHA
UsePcr templateTags3xHAExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2_Firefly_luc-60A
Plasmid#200395PurposeA plasmid IVT template containing a Firefly luciferase CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.DepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIVT-dCas9-mCherry
Plasmid#174443Purposein vitro transcription template for dCas9-mCherryDepositorInsertCas9 Endonuclease Dead
UseIn vitro transcription templateTagsmCherryExpressionMutationPromoterT7 SP6Available sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAT11
Plasmid#204290PurposeFor inserting a phiC31 attP site. HDR integrant is marked with removable ie1-DsRed cassette, which is expressed in abdomen and mouthparts.DepositorInsertie1-DsRed
UseCRISPRTagsExpressionMutationPromoterie1Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_Z17-PDGFR_EF1a_mCherry
Plasmid#186303PurposePlasmid for inducible mammalian cell expression of the Z17 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction.DepositorInsertsZ17-PDGFR
mCherry
UseTagsHA, mycExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P05
Plasmid#120016PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with eGFPDepositorInserteGFP
UsePcr templateTagseGFPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
SaCas9_sgRNA_expression_in_pBluescript
Plasmid#122090PurposeU6 driven SaCas9 sgRNA expression vector for cloning own guidesDepositorInsertSaCas9 tracr (NEWENTRY S. pyogenes, Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-SV40-Galpha-q ONE-GO
Plasmid#204331PurposeGPCR/G protein BRET biosensorDepositorInsertSV40 Galpha-q ONE-GO
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETx-SR6v15
Plasmid#176212PurposeBacterial expression of the SR6v15 nanobody targeting the receptor binding domain of SARS-CoV-2 spike proteinDepositorInsertSR6v15
UseTags6XHisExpressionBacterialMutationPromoterT7Available sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAT62
Plasmid#204301PurposeExpress phiC31 integrase under heat shock control. Insert marked with ie1-EGFP, which expresses in abdomen and mouthparts.DepositorInsertie1-EGFP
UseCRISPRTagsExpressionMutationPromoterie1Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETx-SR6c3
Plasmid#176211PurposeBacterial expression of the SR6c3 nanobody targeting the receptor binding domain of SARS-CoV-2 spike proteinDepositorInsertSR6c3
UseTags6XHisExpressionBacterialMutationPromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
SpCas9_sgRNA_expression_in_pBluescript
Plasmid#122089PurposeU6 driven SpCas9 sgRNA expression vector for cloning own guidesDepositorInsertSpCas9 tracr (NEWENTRY S. pyogenes, Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACT-Lex
Plasmid#183149PurposeYeast-two-hybrid AD vector with 4XLexOp (activated by Lex-ER-B112)DepositorInsertGAL4 AD
UseYeat two-hybridTagsHA tagExpressionYeastMutationPromoterAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
MXS_PGK::tTA2-bGHpA
Plasmid#62448PurposeMXS_chaining vector with PGK::tTA2-bGHpADepositorInsertcassette containing the tetracycline transactivator with PGK promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
ArcLight-S249
Plasmid#36855DepositorInsertfluorescent protein voltage sensor
UseTagsExpressionMammalianMutationCi-VSP contains R217Q mutation; super ecliptic pH…Promotersimian CMVAvailable sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P07
Plasmid#124788PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mNeonGreenDepositorInsertmNeonGreen
UsePcr templateTagsmNeonGreenExpressionMutationPromoterNoneAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV Puro-CCSP:GFP
Plasmid#68487PurposeRetrovirus expressing GFP driven by CCSP promoterDepositorInsertsPuromycin Resistance
GFP
UseRetroviralTagsExpressionMammalianMutationPromoterCCSP and LTRAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only