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Plasmid#169108PurposeExpresses protein G fused to 3 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.DepositorInsertprotein G - NanoLuc - NanoLuc - NanoLuc
UseTagshis-tag and strep-tagExpressionBacterialMutationAla 24 stopPromoterT7Available sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28b-PU.1-ETS
Plasmid#85734PurposeExpresses DNA-binding (ETS) domain of murine PU.1, residues 167-272DepositorInsertETS domain of murine PU.1 (residues 167-272) (Spi1 Human, Mouse)
UseTags6xHis following thrombin cleavage site LVPR|GExpressionBacterialMutationPromoterAvailable sinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MSP1D1deltaH4/2
Plasmid#71712PurposeMembrane scaffold protein (MSP), half of helix 4 truncatedDepositorInsertApolipoprotein A1 (APOA1 Human)
UseTags6xHis and TEV siteExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-103 FL
Plasmid#47090DepositorInsertAL-103 (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET15b-12x
Plasmid#61699PurposeExpresses PTDH mutant Opt12xDepositorInsertOpt12x
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30a-YedK
Plasmid#86884PurposeBacterial expression of E. coli SRAP ortholog YedKDepositorInsertYedK (yedK Eschericia coli)
UseTags6xHis, HA tag, and S-TagExpressionBacterialMutationPromoterAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-soNadV
Plasmid#83363PurposeExpresses nadV from Shewanella oneidensis in Escherichia coliDepositorInsertnadV
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
PCx35IL-pET42
Plasmid#42916DepositorInsertConnexin 35 cytoplasmic loop
UseTags6x His, GST, and S-tagExpressionBacterialMutationcytoplasmic loop is aa 101-179PromoterT7Available sinceMarch 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFS_0354_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2_RC
Plasmid#116966PurposeExpresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2 RC, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETM41-EcPPXc
Plasmid#38329DepositorInsertE. coli polyphosphatase (ppx Escherichia coli)
UseTagsHis, MBP, and TEVExpressionBacterialMutationC-terminus of E. coli polyphosphatase (described …PromoterT7Available sinceAug. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HsSENP6
Plasmid#71469PurposeEncodes human full length SENP6 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).DepositorInsertSUMO1/sentrin specific peptidase 6 (SENP6 Human)
UseTagsHexahistidineExpressionBacterialMutationGene optimised for E. coli expression (Genscript)PromoterT7Available sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET24c(+)-EFTs
Plasmid#31173DepositorInsertE. coli elongation factor Ts
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET21a - Mtb-TF
Plasmid#111513PurposeExpresses M. tuberculosis Trigger Factor with a C-terminal His tagDepositorInsertTrigger Factor
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21a Hbb
Plasmid#26639DepositorInsertHbb (hbb Borrelia burgdorferi)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET28a-hdNadV
Plasmid#83362PurposeExpresses nadV from Haemophilus ducreyi in Escherichia coliDepositorInsertnadV
UseTagsnoneExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET29b-pduCDE
Plasmid#110138PurposeExpresses B12-dependent propanediol dehydratase in E. coli BL21(DE3)DepositorUseTagsExpressionBacterialMutationPromoterT7Available sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pET3a-SF-LMNC-TS
Plasmid#169374PurposeE. coli expression plasmid for human LMNA residues 31-542, corresponding to the shorter lamin C isoform lacking the unstructured head, with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.DepositorInsertLMNA residues 31-542 (LMNA Human)
UseTagsSplit-FlAsH Tag and TwinStrep TagExpressionBacterialMutationdeleted amino acid 1-30 (unstructured head domain…PromoterNative pET3a promotorAvailable sinceMay 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pET3_HMV-His6
Plasmid#164843PurposeExpression of FL human metavinculinDepositorInsertVCL (VCL Human)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only