Addgene

We narrowed to 6,418 results for: lum

Showing: 1261 - 1280 of 6418 results

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EED-F97A

Plasmid

#131326

Purpose

gRNA to generate F97A cage-mutant EED

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

EED-KO-gRNA-2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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EED-KO-gRNA-1

Plasmid

#131321

Purpose

gRNA to knockout EED. Use with EED-KO-gRNA-2

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

EED-KO-gRNA-1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only
pEF1a-T2-GpNLuc

Plasmid

#135949

Purpose

Expresses the GpNLuc LumiFluor targeted to the mitochondira in mammalian cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

GpNLuc LumiFluor

Use

Tags

T2 mitochondrial localization sequence

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pEF1a-T2-OgNLuc

Plasmid

#135951

Purpose

Expresses the OgNLuc LumiFluor targeted to the mitochondira in mammalian cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

OgNLuc LumiFluor

Use

Tags

T2 mitochondrial localization sequence

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pVITRO-Fab_Theo-LUCID

Plasmid

#119048

Purpose

Plasmid encoding the mammalian expression of the Theophiline LUCID based on antibodies (BRET semi-synthetic biosensor)

Depositor

Kai Johnsson

Article

Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2

Inserts

Fab_Theo-LUCID_part1
Fab_Theo-LUCID_part2

Use

Tags

FLAG-tag, His-tag, and Strep-tag

Expression

Mammalian

Mutation

Promoter

EF-1α + CMV/SV40 enhancers and IRES

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pVITRO-Fab_Quin-LUCID

Plasmid

#119049

Purpose

Plasmid encoding the mammalian expression of the Quinine LUCID based on antibodies (BRET semi-synthetic biosensor)

Depositor

Kai Johnsson

Article

Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2

Inserts

Fab_Quin-LUCID_part1
Fab_Quin-LUCID_part2

Use

Tags

FLAG-tag, His-tag, and Strep-tag

Expression

Mammalian

Mutation

Promoter

EF-1α + CMV/SV40 enhancers and IRES

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pVITRO-Fab_MTX-LUCID

Plasmid

#119047

Purpose

Plasmid encoding the mammalian expression of the Metotraxate LUCID based on antibodies (BRET semi-synthetic biosensor)

Depositor

Kai Johnsson

Article

Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2

Inserts

Fab_MTX-LUCID_part1
Fab_MTX-LUCID_part2

Use

Tags

FLAG-tag, His-tag, and Strep-tag

Expression

Mammalian

Mutation

Promoter

EF-1α + CMV/SV40 enhancers and IRES

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-SAC1C389S-HLx8

Plasmid

#108140

Purpose

Inactive SAC1 with extended linker

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)

Use

Tags

Expression

Mammalian

Mutation

SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587)

Promoter

CMV

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pONL-C1_RhoB

Plasmid

#65698

Purpose

Expresses ONL-RhoB in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

RhoB (RHOB Human)

Use

Luciferase

Tags

ONL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pYNL-C1_RhoB

Plasmid

#65696

Purpose

Expresses YNL-RhoB in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

RhoB (RHOB Human)

Use

Luciferase

Tags

YNL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pCNL-N1_vinculin

Plasmid

#65691

Purpose

Expresses Vinculin-CNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

vinculin (Vcl Mouse)

Use

Luciferase

Tags

CNL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only
pONL-N1_vinculin

Plasmid

#65692

Purpose

Expresses Vinculin-ONL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

vinculin (Vcl Mouse)

Use

Luciferase

Tags

ONL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only
pYNL-N1_vinculin

Plasmid

#65690

Purpose

Expresses Vinculin-YNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

vinculin (Vcl Mouse)

Use

Luciferase

Tags

YNL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only
pSEW-Venus-Sec16A-deltaN

Plasmid

#66605

Purpose

Lentiviral vector for express truncated form of Sec16A as a fusion with Venus FP

Depositor

David Stephens

Article

Hughes et al J Cell Sci. 2010 Dec 1;123(Pt 23):4032-8

Insert

Sec16A (SEC16A Human)

Use

Tags

Venus (EYFP derivative)

Expression

Mammalian

Mutation

encodes amino acids 1107-1217 of human Sec16A

Promoter

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
pONL-N1_ITPKA

Plasmid

#65689

Purpose

Expresses ITPKA-ONL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

inositol triphosphate 3-kinase A

Use

Luciferase

Tags

ONL

Expression

Mammalian

Mutation

contains residues 9-52

Promoter

CMV

Available since

July 22, 2015

Availability

Academic Institutions and Nonprofits only

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pYNL-C1_PTS1

Plasmid

#65699

Purpose

Expresses YNL-PTS1 in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

peroxisomal targeting signal 1 (tripeptide SKL)

Use

Luciferase

Tags

YNL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCNL-N1_Golgi

Plasmid

#65694

Purpose

Expresses Golgi-CNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

N-terminal 81 amino acids of beta 1,4-galactosyltransferase

Use

Luciferase

Tags

CNL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCNL-N1_TUBB5

Plasmid

#65679

Purpose

Expresses beta-tubulin-CNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

beta-tubulin class V

Use

Luciferase

Tags

CNL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 17, 2015

Availability

Academic Institutions and Nonprofits only
pYNL-N1_Golgi

Plasmid

#65693

Purpose

Expresses Golgi-YNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

N-terminal 81 amino acids of beta 1,4-galactosyltransferase

Use

Luciferase

Tags

YNL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pYNL-N1_ITPKA

Plasmid

#65687

Purpose

Expresses ITPKA-YNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

inositol triphosphate 3-kinase A

Use

Luciferase

Tags

YNL

Expression

Mammalian

Mutation

contains residues 9-52

Promoter

CMV

Available since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge