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Plasmid#141119PurposeRetroviral overexpression of mutant METTL14 in mammalian cells.DepositorInsertMETTL14 (METTL14 Human)
UseRetroviralTagsExpressionMammalianMutationSubstitution - Missense, position 298, RâžžPPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGF_RT_FAST_Venus
Plasmid#136980PurposeGoldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescenceDepositorInsertseed coat marker, replaced RFP w/ Venus. Replaced NOS terminator w/ Arabidopsis HSP18-2 terminator
UseTagsExpressionBacterialMutationBsaI site domestication in pOLE1:OLE1-RFP 688 G &…PromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGF_RT_FAST_Tq2CFP
Plasmid#136981PurposeGoldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescenceDepositorInsertseed coat marker, replaced RFP w/ Tq2CFP. Replaced NOS terminator w/ Arabidopsis HSP18-2 terminator
UseTagsExpressionBacterialMutationBsaI site domestication in pOLE1:OLE1-RFP 688 G &…PromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSFFV_sfCherry2_TagBFP
Plasmid#83031PurposeExpresses full length sfCherry2 and TagBFP fusion in mammalian cellsDepositorInsertsfCherry+full length_TagBFP
UseLentiviralTagsExpressionMammalianMutation3 mutations in sfCherry full length: E118Q, T128I…PromoterSFFVAvailable sinceJan. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXTm-PH domain
Plasmid#210326PurposeExpresses EGFP with PH domainDepositorInsertPH domain
UseFish expressionTagsExpressionMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
PA-Dre
Plasmid#169706PurposePhotoactivatable Dre recombinaseDepositorInsertDre246-nMag-T2A-pMag-Dre247
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXTm-zebrafish Tspan4a
Plasmid#210327PurposeExpresses Tetraspanin-4a (TSPAN4a) as migrasome markerDepositorInsertTetraspanin 4a (tspan4a Zebrafish)
UseFish expressionTagsExpressionMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
PC1-29-9-2_GAL4-VHL
Plasmid#200434PurposeExpresses the GAL4 DNA Binding domain linked to the VHL E3 Ubiquitin LigaseDepositorInsertGAL4-VHL
UseTagsGAL4, VHLExpressionMammalianMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
PC7-709-28-1_CRBN-VPR
Plasmid#200437PurposeExpresses the VPR transactivation domain linked to the CRBN E3 Ubiquitin LigaseDepositorInsertCRBN-VPR
UseTagsCRBN, VPRExpressionMammalianMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
PA-DreN
Plasmid#169707PurposeThe optimized DreN246-nMagDepositorInsertDre246-nMag
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
PA-DreC
Plasmid#169705PurposeThe optimized pMag-Dre247DepositorInsertpMag-Dre247
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
PC17-605-9-1_BRD4-BD2-VPR
Plasmid#200436PurposeExpresses the VPR transactivation domain linked to the BRD4(BD2) target proteinDepositorInsertBRD4(BD2)-VPR
UseTagsBRD4(BD2), VPRExpressionMammalianMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
PC15-421-14-1_TRIM24-BD-VPR
Plasmid#200435PurposeExpresses the VPR transactivation domain linked to the TRIM24(BD) target proteinDepositorInsertTRIM24(BD)-VPR
UseTagsTRIM24(BD), VPRExpressionMammalianMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL
Plasmid#196286Purpose35S promoter-driven expression of the positive-sense TSWV L RNA segment encoding codon-optimized L (RdRp)DepositorInsertFull length TSWV L antigenome encoding codon-optimized L (RdRp)
UseCRISPRTagsExpressionPlantMutationPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-G-Flamp1
Plasmid#188567Purposegreen biosensor for cAMP in mammalian cellsDepositorInsertG-Flamp1
UseTagsHis tagExpressionMammalianMutationPromoterCAGAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
bNS2B47NS3
Plasmid#155320Purposeco-expression of DENV4 47 peptides from NS2B cofactor and NS3 full lengthDepositorInsertCo expression of DENV4 NS3 full length protein with 47 peptides from NS2B cofactor
UseTagsHis tagExpressionBacterialMutationWTPromoterT7Available sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
RBD#4L
Plasmid#193021PurposehnRNP C RRM RNA binding domain inserted in between N415 and N416 of Loop 1 of LwaCas13aDepositorInsertCas13a
UseTagshnRNP C RRMExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
RBD#3L
Plasmid#193020PurposehnRNP A1 RRM2 RNA binding domain inserted in between N415 and N416 of Loop 1 of LwaCas13aDepositorInsertCas13a
UseTagshnRNP A1 RRM2ExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MCS-13 x Linker-BioID2-HA-GPI-PSS
Plasmid#194750PurposeTo fuse your protein of interest to the N-terminus of GPI-anchored-BioID2DepositorTypeEmpty backboneUseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
CALID
Plasmid#169708PurposeCre-activated light-inducible Dre recombinaseDepositorInserta double-floxed inverted Dre246-nMag-T2A-pMag-Dre247
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only