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Plasmid#133060PurposeLentiviral vector with a (Ubiquitous Chromatin Opening Element) UCOE upstream of a CAG promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-mScarlet-I fusion protein.DepositorInsertTau.K18(LM)
UseLentiviralTagsmScarletIExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
p6XHis_NLS-SaCas9
Plasmid#101086PurposeExpress SaCas9 in bacteria with a 6xHis tag for purificationDepositorInsertCRISPR-associated protein Cas9/Csn1
UseCRISPRTagsTrxA-6xHis-STagExpressionBacterialMutationPromoterT7lacAvailable sinceDec. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
p5E-3x125bp-mnx1
Plasmid#169043Purpose5' entry vector for Gateway cloning for expression in spinal motor neurons using 3 tandem repeats of the zebrafish mnx1 enhancer.DepositorInsertMnx1 enhancer (3x) (mnx1 Zebrafish)
UseGateway entry cloning vectorTagsExpressionMutationPromoter3xMnx1Available sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
CLYBL-TO-PAX7
Plasmid#204346PurposeKnock-in vector to insert a dox-inducible PAX7 transgene into the CYBL locus in human pluripotent stem cells. PAX7 induction results in myogenic differentiation.DepositorInserthuman PAX7
UseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
SID4x-dCas9-KRAB
Plasmid#106399PurposeExpresses catalytically inactive dCas9 with N-terminal fusion of Sin3a Interacting Domain (SID) and C-terminal fusion of repressive KRAB domainDepositorUseTagsFLAG, HA, and NLSExpressionMammalianMutationPromoterAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET30a-SARS-CoV2-NP-Nt(47-173)
Plasmid#165091Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal(47-173) (N SARS-CoV2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
Lenti-sgNT.2#2/Cre
Plasmid#173661PurposeExpresses a NT.2-targeting gRNA and Cre-recombinaseDepositorInsertNon-targeting gRNA
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMutationPromoterAvailable sinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET32a-Spike-S-4P
Plasmid#165094Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 spike protein site 550-570, 785- 805, 810-830 and 1146 1166 (S SARS-CoV2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 2, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET30a-SARS-CoV2-ORF3b (S23Q)
Plasmid#165088Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 ORF3b (S23Q)
UseTagsExpressionBacterialMutationStop codon to glutamine at 23rd amino acidPromoterAvailable sinceFeb. 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
Lenti-BaeI-Pgk-Cre
Plasmid#173663PurposeVector with BaeI site to allow cloning of custom sgRNA into vector containing Cre-recombinaseDepositorInsertBaeI site
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMutationPromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET32a-Spike-403-505
Plasmid#165095Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 spike protein site 403-505 (S SARS-CoV2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRCIscript-crhr1_TAL1B
Plasmid#44235DepositorInsertcrhr1 pTAL scaffold 1B (crhr1 Zebrafish)
UseMrna transcription vectorTagsExpressionMutationPromoterT3Available sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRCIscript-crhr1_TAL1A
Plasmid#44234DepositorInsertcrhr1 pTAL scaffold 1A (crhr1 Zebrafish)
UseMrna transcription vectorTagsExpressionMutationPromoterT3Available sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
GoldyTALEN-ppp1cab TAL1
Plasmid#41835DepositorInsertprotein phosphatase 1, catalytic subunit, alpha isoform b (ppp1cab Zebrafish)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJan. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgNT.3#3/Cre
Plasmid#173662PurposeExpresses a NT.3-targeting gRNA and Cre-recombinaseDepositorInsertNon-targeting gRNA
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMutationPromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRD403
Plasmid#163108PurposeExpression of mScarlet-I_H-NSdbd in Escherichia coli (and, potentially, other bacteria)DepositorInsertmScarlet-I_H-NSdbd
UseTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
GoldyTALEN-ppp1cab TAL2
Plasmid#41836DepositorInsertprotein phosphatase 1, catalytic subunit, alpha isoform b (ppp1cab Zebrafish)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-ER WT
Plasmid#49498PurposeExpresses HA-tagged ER wild-type in mammalian cellsDepositorInsertESR1 (ESR1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-ChR2-YFP
Plasmid#204345PurposePiggyBac transposon vector suitable for inserting the ChR2-YFP optogenetic actuator driven by the ubiquitous CAG promoter into the genome of mammalian cell lines.DepositorInsertChR2-YFP
UseTagsYellow fluorescent proteinExpressionMammalianMutationC-terminus (aa315-737) is deleted - PMID: 1461559…PromoterCAGAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-hBMPR-1A CA
Plasmid#49527Purposeexpresses constitutively active human BMP receptor 1ADepositorInsertBMPR-1A CA (BMPR1A Human)
UseTagsExpressionMammalianMutationconstitutively active mutation (Q233D); also P2A …PromoterCMVAvailable sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only