Skip to main content
Addgene
Showing: 1261 - 1280 of 3905 results
  1. pcDNA3-EngPDm

    Plasmid
    #64040
    Purpose
    Mammalian expression of mutant paired domain of Pax8 fused to the N-terminal repressor domain of the drosophila engrailed protein (Eng)
    Depositor
    Insert
    Pax8 paired domain (Pax8 Mouse)
    Use
    Tags
    Eng (N-terminal repressor domain of the drosophi…
    Expression
    Mammalian
    Mutation
    paired domain, aa 1-147, with aa 30 to 32 changed…
    Promoter
    CMV
    Available since
    April 23, 2015
    Availability
    Academic Institutions and Nonprofits only
  2. HTR1A-Tango

    Plasmid
    #66404
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Insert
    HTR1A (HTR1A Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
  3. pH-nCas9-PPE

    Plasmid
    #140447
    Purpose
    For plant prime editing in rice plants or monocotyledons protoplasts
    Depositor
    Insert
    nCas9(H840A)-M-MLV
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    H840A for Cas9; D200N, T306K, W313F, T330P and L…
    Promoter
    maize Ubiquitin-1, OsU3
    Available since
    May 5, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. Cav3P104L-YFP

    Plasmid
    #68404
    Purpose
    Expression of fluorescently tagged Caveolin3 point mutation in mammalian cells
    Depositor
    Insert
    Cav3 (Cav3 Mouse)
    Use
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    Proline at position 104 changed to a Leucine
    Promoter
    CMV
    Available since
    Sept. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
  5. ANGPT-bio-His

    Plasmid
    #53404
    Purpose
    Expresses full-length Angiopoietin-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Insert
    ANGPT (ANGPT1 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
  6. p426_Cas9_gRNA-YPRCd15c

    Plasmid
    #87404
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting YPRCd15c sequence AATCCGAACAACAGAGCATA in yeast chromosome 14.
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting YPRCd15c
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 1261 - 1280 of 3905 results