1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. pUASp YFP Rho1 DN

   Plasmid

   #52231

   Purpose

   Drosophila expression of YFP-Rho1 dominant negative mutant

   Depositor

   Matthew Scott

   Insert

   Rho1 (Rho1 Fly)
   

   Use

   Drosophila

   Tags

   YFP

   Expression

   Insect

   Mutation

   T19N; DN mutation

   Promoter

   GAL4

   Available since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pCAG-sfGFP-GSAx9-iCre-ERT2

   Plasmid

   #125748

   Purpose

   sfGFP and iCreERT2 fused by GSAx9 linker

   Depositor

   Mario Capecchi

   Article

   Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

   Insert

   sfGFP-GSAx9-iCre-ERT2
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p426-SNR52p-gRNA.clr-2.Y-SUP4t

   Plasmid

   #89692

   Purpose

   gRNA for clr-2 locus

   Depositor

   Christian Hong

   Article

   Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

   Insert

   gRNA for clr-2 locus
   

   Use

   CRISPR; Grna

   Tags

   No

   Expression

   Mutation

   Promoter

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG-TetR-RNF169UBD

   Plasmid

   #119011

   Purpose

   Mammalian expression of a fusion protein of single chain Tet repressor with the human RNF169 Ubiquitin binding domain and BFP expression

   Depositor

   Ralf Kuehn

   Article

   Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

   Insert

   TetR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PSUC2-GFPantisense-CYC1t-pRS413

   Plasmid

   #64404

   Purpose

   SUC2 regulated expression of an antisense RNAi construct for GFP

   Depositor

   Claudia Vickers

   Article

   Williams et al Microb Cell Fact. 2015 Apr 1;14:43. doi:

   Insert

   GFPantisense
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SUC2

   Available since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PABE-2

   Plasmid

   #115623

   Purpose

   Targeted A to G in wheat plants or monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Li et al Genome Biol. 2018 May 29;19(1):59. doi:

   Insert

   wtTadA-TadA7.10-nCas9-NLS
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLenti-CMV-SMCHD1-R1868*-3xFLAG

   Plasmid

   #130679

   Purpose

   Expresses human SMCHD1 R1868* with a C-terminal 3xFLAG tag

   Depositor

   Stephen Tapscott

   Article

   Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

   Insert

   SMCHD1 R1868* (SMCHD1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   R1868_V2005delinsX

   Promoter

   CMV

   Available since

   June 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBN79

   Plasmid

   #86717

   Purpose

   DamID plasmid for C. elegans

   Depositor

   Peter Askjaer

   Article

   Gonzalez-Aguilera et al Genome Biol. 2014 Feb 3;15(2):R21. doi:

   Insert

   Phsp16.41::Dam::MYC::Emr-1
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Feb. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBLIC-neo

   Plasmid

   #45198

   Purpose

   a ligation-independent cloning (LIC) retroviral vector for stable gene transduction in mammalian cells. Neo selection.

   Depositor

   Priyamvada Rai

   Article

   Patel et al BMC Biotechnol. 2012 Jan 16;12:3. doi: 1

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 19, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pET19b_4CL:P5SHb + STS:P6SHb

    Plasmid

    #139792

    Purpose

    expression of biosynthetic enzymes 4CL and STS in fusion with CC

    Depositor

    Roman Jerala

    Article

    Fink et al AMB Express. 2020 May 24;10(1):97. doi:

    Inserts

    4CL:P5SHb
    STS:P6SHb
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET19b_4CL:P3S + STS:AP4S

    Plasmid

    #139795

    Purpose

    expression of biosynthetic enzymes 4CL and STS in fusion with CC

    Depositor

    Roman Jerala

    Article

    Fink et al AMB Express. 2020 May 24;10(1):97. doi:

    Inserts

    4CL:P3S
    STS:AP4S
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEL089

    Plasmid

    #137897

    Purpose

    iSTU-CRISPR/Cas12a HH-HDV structure crRNA insertion vector with OsCDPK2 intron

    Depositor

    Yong Zhang

    Article

    Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti-CMV-SMCHD1-Exon37-48-3xFLAG

    Plasmid

    #130688

    Purpose

    Expresses human SMCHD1 Exon37-48 with a C-terminal 3xFLAG tag

    Depositor

    Stephen Tapscott

    Article

    Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

    Insert

    SMCHD1 Exon37-48 (SMCHD1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    1M_T1522delinsM

    Promoter

    CMV

    Available since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti-CMV-SMCHD1-Exon1-36-3xFLAG

    Plasmid

    #130681

    Purpose

    Expresses human SMCHD1 Exon1-36 with a C-terminal 3xFLAG tag

    Depositor

    Stephen Tapscott

    Article

    Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

    Insert

    SMCHD1 Exon1-36 (SMCHD1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D1523_V2005delinsX

    Promoter

    CMV

    Available since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFH99

    Plasmid

    #128204

    Purpose

    classic sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    classic sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFH84

    Plasmid

    #128195

    Purpose

    Level1 ZmUBIp::A3A-PBE (wheat codon optimized):NOSt

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    ZmUBIp::A3A-PBE (wheat codon optimized):NOSt
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFH100

    Plasmid

    #128205

    Purpose

    improved sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    improved sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTC151

    Plasmid

    #70015

    Purpose

    TALENs targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a non-replicating, mutant version of the Gemini Viral Replicon

    Depositor

    Daniel Voytas

    Article

    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

    Insert

    Nuclease (TALENs) + Donor + GVR
    

    Use

    TALEN

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFH82

    Plasmid

    #128193

    Purpose

    Level1 ZmUBIp::enCas9-Poll3M-TBD (wheat codon optimized):NOSt

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    ZmUBIp::enCas9-Poll3M-TBD (wheat codon optimized):NOSt
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFH93

    Plasmid

    #128196

    Purpose

    Level1 ZmUBIp::nScCas9-ABE7.10 (wheat/human codon optimized):NOSt

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    ZmUBIp::nScCas9-ABE7.10 (wheat/human codon optimized):NOSt
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge