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Plasmid#205268PurposeHuman expression vector for SpuFz1 omegaRNA 5' +275 nt extended scaffold (BbsI guide entry site)DepositorInsertSpuFz1 omegaRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pME-cas9-2A-mCherry
Plasmid#107592PurposeAn entry vector with cas9 -2A-mCherryDepositorInsertcas9-2A-mCherry
UseCRISPRTags2A-mCherryExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSSM16: pCMV-HA-NLS-SpuFz1
Plasmid#205271PurposeHuman expression vector for human codon optimized N-terminal NLS-tagged SpuFz1DepositorInsertSpuFz1
UseTagsHA, NLS(Makkerh)ExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMJ548: pU6-SpuFz1_MS2-omegaRNA(75nt)-entry
Plasmid#205269PurposeHuman expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (BbsI guide entry site)DepositorInsertSpuFz1 omegaRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-cdc2-AF (718)
Plasmid#39872DepositorInsertCdc2 (CDK1 Human)
UseTagsExpressionMammalianMutationAF = T14A, Y15FPromoterCMVAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pegRNA_LMNA-1
Plasmid#199273PurposepegRNA used to induce LMNA (c.1824C > T) mutationDepositorInsertLMNA 1824CtoT pegRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMJ529: pU6-SpuFz1_MS2-omegaRNA(75nt)-Guide(B2M)
Plasmid#205278PurposeHuman expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (Guide:B2M)DepositorInsertSpuFz1 omegaRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMJ106: pRS426-His-MBP-NlovFz2_Native
Plasmid#205253PurposeS. cerevisiae expression vector for NlovFz2 native guideDepositorInsertNlovFz2
UseTags10xHis, MBPExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
PE-Cas9
Plasmid#174851PurposeExpress PE-Cas9 in mammalian cellsDepositorInsertPE-Cas9
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMJ45: pRS426-His-MBP-SpuFz1_Native
Plasmid#205252PurposeS. cerevisiae expression vector for SpuFz1 native guideDepositorInsertSpuFz1
UseTags10xHis, MBPExpressionYeastMutationPromoterAvailable sinceSept. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMEH373 dTomato 3x high affinity MS2 loops
Plasmid#168221PurposeMammalian expression of dTomato with 3 high affinity MS2 loopsDepositorInsertdTomato
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC-GoldyTALEN
Plasmid#38143Purposedestination vector for the Golden Gate TALEN kit, directs expression of TALENs from a truncated CAGs promoterDepositorInsertGoldyTALEN
UseTagsAcV5 and FokI homodimerExpressionMammalianMutationTruncate at N and C terminousPromoterminiCaggsAvailable sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGFP-E p27 (CC#631)
Plasmid#15192DepositorInsertp27 (Cdkn1b Mouse)
UseRetroviralTagsFlag and HisExpressionMammalianMutationPromoterAvailable sinceJune 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMSSM151: pU6-NlovFz2_omegaRNA-entry
Plasmid#205263PurposeHuman expression vector for NlovFz2 omegaRNA (BsmBI guide entry site)DepositorInsertNlovFz2 omegaRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHis-zfCSF3a
Plasmid#65612Purposeexpress soluble His-tagged zebrafish CSF3a/Gcsfa in E. coliDepositorInsertCsf3a (csf3a Zebrafish)
UseTags(His)6ExpressionBacterialMutationPromoterT7Available sinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMJ123: pRS426-His-MBP-SpuFz1_PSP1
Plasmid#205256PurposeS. cerevisiae expression vector for SpuFz1 reprogrammed guide (targeting PSP1)DepositorInsertSpuFz1
UseTags10xHis, MBPExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
RCIscript-GoldyTALEN
Plasmid#38142Purposedestination vector for the Golden Gate TALEN kit, for in vitro synthesis of TALEN mRNAsDepositorInsertGoldyTALEN
UseMrna transcription vectorTagsAcV5 and FokI homodimerExpressionMutationTruncate at N and C terminousPromoterT3Available sinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMJ333: pU6-MmeFz2_omegaRNA-entry
Plasmid#205267PurposeHuman expression vector for MmeFz2 omegaRNA (BbsI guide entry site)DepositorInsertMmeFz2 omegaRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI-J23106-geneIII
Plasmid#80843PurposePhagemid (encodes M13 gene III and lambda cIopt).DepositorInsertsM13 gene III
Lambda cI
UseTagsExpressionBacterialMutationPromoterBBa_J23106 and rpoNAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMJ240: pRS426-His-MBP-MmeFz2_Native
Plasmid#205255PurposeS. cerevisiae expression vector for MmeFz2 native guideDepositorInsertMmeFz2
UseTags10xHis, MBPExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only