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pCI-Puro_hCOL4A4-Myc-His
Plasmid#198093PurposeExpress hCOL4A4 in mammalian cellsDepositorInsertCOL4A4 (COL4A4 Human)
UseTagsTEV-Myc-HisExpressionMammalianMutationPromoterCMV, beta-globin/IgG chimeric intronAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_mouse_Adar1_ccC
Plasmid#158121Purposedeletion mAdar1DepositorInsertAdar1 (Adar Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-GFP_mmu-miR-16-5p_sponge
Plasmid#132624PurposeExpresses a TurboGFP transcript with microRNA sponge for mmu-miR-16-5p as 3'UTRDepositorInsertstop codon, mmu-miR-16-5p sponge sequence and polyA signal
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT2MPEF-NLS-QUE37C
Plasmid#129326PurposeATP measurement, nucleus, Tol2 transposonDepositorInsertQUEEN-37C
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
ELL3 Streptag CtoA
Plasmid#127283PurposeExpresses mouse ELL3 with streptactin tag; To interrogate if the mutation decreases putative Myc activityDepositorInsertELL3 (NM_145973) (ELL3 Mouse)
UseTagsTwin strep-tagExpressionMammalianMutationCEYLHNK to AEYLHQKPromoterEF1aAvailable sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
ELL1 Streptag
Plasmid#127278PurposeExpresses mouse ELL1 with streptactin tag in mammalian cellsDepositorInsertELL1 (NM_007924) (ELL Mouse)
UseTagsTwin strep-tagExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-V5-Ccdc167
Plasmid#120242PurposeExpresses V5-tagged Ccdc167 in lentiviral vectorDepositorInsertCcdc167 (Ccdc167 Mouse)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-SapI
Plasmid#196078Purpose(Empty Backbone) Constitutive CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.DepositorInsertsdCas9-KRAB-MeCP2 repressor
hU6 promoter and gRNA scaffold
UseTagsExpressionMammalianMutationCatalytically dead mutant of the Cas9 endonucleas…PromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
lenti-sgRNA puro
Plasmid#104990PurposeThis 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and puromycin resistance from an EF-1a promoter.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP
Plasmid#132393PurposeExchange cassette containing inducible TauP301L 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 siteDepositorInserthuman TauP301L 2N4R-EGFP (MAPT Synthetic, Human)
UseCRISPR and Cre/LoxTagsEGFPExpressionMammalianMutationProline 301 to Leucine (P301L)PromoterTREtightAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
SF-SON IRES Puro
Plasmid#214402PurposeExpresses human SOX10, OLIG2, NKX6.2 and PAC (puromycin N-acetyl-transferase)DepositorUseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE
Plasmid#85452PurposeLentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.DepositorInsertU6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro
UseCRISPR and LentiviralTagsNLSExpressionMammalianMutationPromoterAvailable sinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting
Plasmid#92139PurposeTargeting vector to introduce a Fucci cell cyle reporter cassette mCherry(G1)/BFP(S/G2) at the mouse TIGRE acceptor locus using puromycin selectionDepositorUseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-RAP1A-WT-puro
Plasmid#156166PurposeLentiviral vector for expression of RAP1A-WT, with puromycin selectionDepositorInsertRAP1A (RAP1A Human)
UseLentiviralTagsnoneExpressionMammalianMutationwild type formPromoterhuman PGKAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICE-EGFP-FLAG-PLIN3
Plasmid#161918PurposeExpresses human PLIN3 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.DepositorInsertPerilipin-3 (PLIN3 Human)
UseTagsGFP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
TRIPZ-CTID195-GSQ-UBnc-PURO
Plasmid#208037PurposeEnables inducible expression of C-terminal Split-TurboID-fused UBnc, to perform Ubiquitin-ID; nc = non-cleavable mutation; selection with puromycinDepositorInsertUbnc (UBC Human)
UseLentiviralTagsMyc, C-terminal Split-TurboIDExpressionMutationL73P Mutation near C-terminus suppresses cleavage…PromotertetO/UBCAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DEST (w118-1) eGFP
Plasmid#107505PurposeExpresses eGFP, puromycin resistantDepositorInserteGFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only
