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Plasmid#20389DepositorInsertCFP-reporter
UseTagsExpressionYeastMutationX=UbiY (see schematic)PromoterAvailable sinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pNC987
Plasmid#20404DepositorInsertUAS cassette (FUS1)
UseTagsExpressionYeastMutationX= M (see schematic)PromoterAvailable sinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pNC825
Plasmid#20385DepositorInsertUAS cassette (GAL1,10)
UseTagsExpressionYeastMutationX=UbiY (see schematic)PromoterAvailable sinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pNC840
Plasmid#20387DepositorInsertUAS cassette (GAL1,10)
UseTagsExpressionYeastMutationX=UbiM (see schematic)PromoterAvailable sinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pNC866
Plasmid#20395DepositorInsertUAS cassette (GAL1,10)
UseTagsExpressionYeastMutationX=UbiE (see schematic)PromoterAvailable sinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pNC948
Plasmid#20399DepositorInsertCFP-reporter
UseTagsExpressionYeastMutationX=UbiM (see schematic) linker=deltakPromoterAvailable sinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pX330A-FBL/PITCh
Plasmid#63671PurposeExpresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNADepositorInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBBK21 pCAS-Tyr-[gRNA: 4=XII-5] (SplitHygR, AmpR)
Plasmid#179005PurposeSp.Cas9 and gRNA yeast expression vector withXII-5 gRNA pre-cloned. Selection =SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK20 pCAS-Tyr-[gRNA: 3=XII-2] (SplitHygR, AmpR)
Plasmid#179004PurposeSp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK03 pCAS-Tyr-[gRNA: BsaI GFP dropout] (HygR,AmpR)
Plasmid#178987PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Hygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK04 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitHygR,AmpR)
Plasmid#178988PurposeSp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOsU3
Plasmid#170132PurposeFor sgRNA expression in monocotyledons protoplastsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBBK95 pCAS-Tyr-[gRNA: 2xBsaI+NotI cut sites] (HygR)
Plasmid#179079PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Hygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK01 pCAS-Tyr-[gRNA: BsaI GFP dropout] (KanR, AmpR)
Plasmid#178985PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330 EGFR-sgRNA
Plasmid#188633PurposeA human codon-optimized SpCas9 and chimeric guide RNA targeting C-terminus of human EGFRDepositorInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK05 pCAS-Tyr-[gRNA: BsaI GFP dropout] (NatR,AmpR)
Plasmid#178989PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Nourseothricin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK94 pCAS-Tyr-[gRNA: 2xBsaI+NotI cut sites] (KanR)
Plasmid#179078PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
EZ-L439
Plasmid#169146PurposeEncodes OptoINVRT7 circuitDepositorInsertVP16-EL222
UseTagsSV40 NLSExpressionYeastMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXCas9H840A
Plasmid#60900PurposeDual Expression Vector for Cas9 H840A nickase and gRNADepositorTypeEmpty backboneUseCRISPRTags3XFLAGExpressionMammalianMutationPromoterCBh and U6Available sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
KG#65
Plasmid#110933PurposeExpresses proteins in cholinergic neurons in the ventral nerve cord of C. elegansDepositorInsertsunc-17 beta promoter
unc-54 3' control region
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only