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Plasmid#19714DepositorUseTagsExpressionMammalianMutationmouse Brn2 DNA binding domain-Drosophila engraile…PromoterAvailable sinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only
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pIRTetR-GAL80
Plasmid#185853PurposePlugging the mechanism of tetracycline-mediated repression of GAL promoters in Saccharomyces cerevisiaeDepositorInsertgal80Arm1-PAgTEF1-KanMX4-TAgTEF1-PHAC1>TetR>TUP1>PABF1- PTEF1+[TetO]>GAL80Arm2
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4D41
Plasmid#185841PurposeTesting the CYC1 core promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PCYC1+[Z268]>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10CF72 (B11)
Plasmid#185859PurposeTesting the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M3(2*PZ4)>(BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP8EFA
Plasmid#185854PurposeTesting a heat inducible protein degradation tag -DHFR P66L using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1>UBI4>RHGSGTMV>DHFRP66L>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationDHFR P66LPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.LSL.tdTomato
Plasmid#100048PurposeAAV mediated Cre-dependent expression of tdtomato (Lox-Stop-Lox)DepositorInserttdtomato
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcS-RDG-C1C2
Plasmid#200163PurposeThis plasmid encodes non-binder control monobody (RDG) fused to the extracellular vesicle binding domain (C1C2) of lactadherin for surface engineering extracellular vesicles.DepositorInsertsRDG-C1C2
C1C2 domain of lactadherin
UseTagsHA and HISExpressionMammalianMutationsequence adopted from doi: 10.1371/journal.pone.0…PromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcS-E626-C1C2
Plasmid#200162PurposeThis plasmid encodes EGFR targeting monobody (E626) fused to the extracellular vesicle binding domain (C1C2) of lactadherin for surface engineering extracellular vesicles.DepositorInsertsE626
C1C2 domain of lactadherin
UseTagsHA and HISExpressionMammalianMutationsequence adopted from doi: 10.1371/journal.pone.0…PromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-FLEX-tdTomato-WPRE
Plasmid#51503PurposeCan be used to generate AAV virus that will express tdTomato in the presence of CreDepositorInserttdTomato
UseAAVTagsExpressionMutationPromoterpCAGAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1(S)-tdTomato-WPRE
Plasmid#51506PurposeCan be used to generate AAV virus that will express tdTomato in neurons from the synapsin promoterDepositorHas ServiceAAV5InserttdTomato
UseAAVTagsExpressionMutationPromoterphSyn1Available sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.LSL.EGFP
Plasmid#100047PurposeAAV mediated Cre-dependent expression of EGFP (Lox-Stop-Lox)DepositorInsertEGFP
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmiRA1-MYB
Plasmid#141120PurposeLentiviral overexpression of MYB in mammalian cells.DepositorInsertMYB (MYB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGS-CD64-2
Plasmid#109191PurposeEncodes full-length engineered CD64 with C-terminal Twin-Strep tag to be expressed in a baculovirus/insect cell expression systemDepositorInsertFull-length engineered CD64, derived from human CD64
UseTagsTwin-StrepExpressionInsectMutationengineered for efficient expression, see publicat…PromoterPolyhedrinAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGGC_RT_mScarlet
Plasmid#136967PurposeGoldengate/Greengate CDS module, containing mScarletDepositorInsertmScarlet
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGD_RT_SRDX
Plasmid#136976PurposeGoldengate/Greengate module EAR-repression domainDepositorInsertSRDX
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGC_RT_Tq2CFP
Plasmid#136969PurposeGoldengate/Greengate CDS module, containing Tq2CFP.DepositorInsertTq2CFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGD_RT_2NLS
Plasmid#136977PurposeGoldengate/Greengate module nuclear localization signalDepositorInsertSV40 NLS
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-FLEX-EGFP-WPRE
Plasmid#51502PurposeCan be used to generate AAV virus that will express EGFP in the presence of CreDepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertEGFP
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1(S)-FLEX-tdTomato-WPRE
Plasmid#51505PurposeCan be used to generate AAV virus that will express tdTomato in the presence of Cre in neurons from the synapsin promoterDepositorInserttdTomato
UseAAVTagsExpressionMutationPromoterphSyn1Available sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1(S)-FLEX-EGFP-WPRE
Plasmid#51504PurposeCan be used to generate AAV virus that will express EGFP in the presence of Cre in neurons from the synapsin promoterDepositorInsertEGFP
UseAAVTagsExpressionMutationPromoterphSyn1Available sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only