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Plasmid#184832PurposeExpresses human KIF5A(∆exon27) and human KLC1 in insect cellsDepositorUseCre/LoxTagsHis-FLAG and mScarlet-2xStrepIIExpressionInsectMutation∆exon27 form of human KIF5APromoterp10 and polyhedrinAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only
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pOmnibac_ZZ_linker_K963_GFP_SNAP
Plasmid#159727PurposeExpresses kinesin-1 (amino acids 1-963) in Sf9 cells with a C-terminal GFP and SNAP tagDepositorInsertKIF5B (full length, 1-963 amino acids), K963 (KIF5B Human)
UseTagsGFP and SNAP tags and ZZ affinity tagExpressionInsectMutationPromoterAvailable sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFl Strep-sumo-TEV-hAgo2 D597N
Plasmid#136236PurposeExpressing catalytically inactive human Argonaute-2 in insect cellsDepositorInserthuman Argonaute-2 (AGO2 Human)
UseTagsSterp SumoExpressionInsectMutationD597NPromoterpolyhedrinAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR207 SARS-CoV2 TEV-NSP3
Plasmid#154402PurposeGateway-compatible Entry vectorDepositorInsertNSP3 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vector, with insert of n…TagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceSept. 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR-BLINK2
Plasmid#117075PurposeBLINK2 gene in pDONR vector for Gateway cloning systemDepositorInsertBLINK2
UseGateway cloning systemTagsExpressionMutationglutamine 112 changed into aspartate (original LO…PromoterAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-Ma-Acridone-RS1 (A7)
Plasmid#197566PurposeExpression of Methanomethylophilus alvus (Ma) Acridone RS1 (A7) tRNA synthetase/Pyl-tRNA pair for the encoding of Acridone at TAG codons in HEK293 cellsDepositorInsertsM. alvus PylRS - Acd RS1 (A7)
M. alvus Pyl-tRNA (6) (4x copies)
UseTagsNoneExpressionMammalianMutationN166A, V168G, W239CPromoterCMV and U6/H1Available sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOmnibac_ZZ_linker_K560_GFP_SNAP
Plasmid#159720PurposeExpresses kinesin-1 (amino acids 1-560) in Sf9 cells with a C-terminal GFP and SNAP tagDepositorInsertKIF5B (only amino acids 1-560), K560 (KIF5B Human)
UseTagsGFP and SNAP tags and ZZ affinity tagExpressionInsectMutationOnly amino acids 1-560PromoterAvailable sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR207 SARS-CoV-2 NSP1_nostop
Plasmid#149304PurposeGateway-compatible Entry vector, with insert of NSP1 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.DepositorInsertNSP1 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceMay 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR207 SARS-CoV-2 E_nostop
Plasmid#149321PurposeGateway-compatible Entry vector, with insert of the envelope (E) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.DepositorInsertE (E SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR207 SARS-CoV-2 ORF3A_nostop
Plasmid#149319PurposeGateway-compatible Entry vector, with insert of ORF3A gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.DepositorInsertORF3A (ORF3a SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
ssAAV-EF1a-FLuc-WPRE-HgHpA_bac_293
Plasmid#118412PurposeExpresses firefly luciferase under the control of a strong ubiquitous promoter EF1a along with a WPRE cassette. Backbone is compatible with both baculoviral and human production methods of AAV.DepositorInsertFirefly luciferase
UseAAV, Luciferase, and Synthetic BiologyTagsExpressionInsect and MammalianMutationPromoterEF1aAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR207 SARS-CoV-2 M_nostop
Plasmid#149322PurposeGateway-compatible Entry vector, with insert of the membrane (M) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.DepositorInsertM (M SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR207 SARS-CoV-2 ORF7A_nostop
Plasmid#149324PurposeGateway-compatible Entry vector, with insert of ORF7A gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.DepositorInsertORF7A (ORF7a SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBFC0694
Plasmid#186693PurposeShDART under control of Lac promoter, original configuration (pre-gRNA terminator and J23119 promoter), Sh_lacZ_α_1 gRNA, GmR+sfGFP cargoDepositorInsertstnsB
tnsC
tniQ
Cas12k
Sh_lacZ_α_1 gRNA
Tn7 transposon
GmR+sfGFP ShDART Cargo
UseTagsExpressionBacterialMutationPromoterPLacAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR207 SARS-CoV-2 E 27nt-del
Plasmid#153954PurposeGateway-compatible Entry vector, with insert of E CDS bearing a 27nt deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020DepositorInsertE (E SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJune 9, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLIB_GST-nsp13 (SARS-CoV-2)
Plasmid#169190PurposeBaculoviral transfer vector to express GST-nsp13 (SARS-CoV-2) in insect cellsDepositorInsertGST-nsp13 (ORF1ab SARS-CoV-2, Synthetic)
UseTagsGSTExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR207 SARS-CoV-2 E 27nt-del_nostop
Plasmid#153955PurposeGateway-compatible Entry vector, with insert of E CDS bearing a 27nt deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020DepositorInsertE (E SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJune 23, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMMACE DEST CMV PE2
Plasmid#206276PurposeDEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes PE2 under the control of CMV promoter. CRE-Acceptor in MultiBac system.DepositorInsertPE2
UseCRISPR; Recombinant baculovirus production, multi…TagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFB-CG-LYCHOS WT-HA-GFP
Plasmid#199687PurposeExpression of LYCHOS WT in insect cellsDepositorInsertLYCHOS (GPR155 Human)
UseTags10xHis, EGFP, HA, HRV 3C, and HisExpressionInsectMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOmnibac_ZZ_linker_K490_GFP_SNAP
Plasmid#159721PurposeExpresses kinesin-1 (amino acids 1-490) in Sf9 cells with a C-terminal GFP and SNAP tagDepositorInsertKIF5B (only amino acids 1-490), K490 (KIF5B Human)
UseTagsGFP and SNAP tags and ZZ affinity tagExpressionInsectMutationOnly amino acids (1-490)PromoterAvailable sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only