pDual
Plasmid
#207883
Purpose
Expresses thermosensitive variants of the homing endonucleases I-OnuI and I-GpeMI, each in a different open reading frame. Both were made thermosensitive by a fusion with the L212P VMA1 intein.
Depositor
Inserts
Thermosensitive fusion of I-OnuI and the L212P VMA1 intein.
Thermosensitive fusion of I-GpeMI and the L212P VMA1 intein.
Thermosensitive fusion of I-GpeMI and the L212P VMA1 intein.
Use
Synthetic Biology
Tags
Expression
Bacterial
Mutation
Leucine 212 changed to proline, leucine 434 in th…
Promoter
Available since
Aug. 29, 2024
Availability
Academic Institutions and Nonprofits only
