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Plasmid#214075PurposeLuminescent reporter system to measure EJC-independent NMD activity. Intron present in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationLinker sequence present between firefly luciferas…PromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
LiRT_RTctrl
Plasmid#214078PurposeLuminescent reporter system, readthrough control, NMD negative control. Intron present in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationLinker sequence present between firefly luciferas…PromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
LiRTi
Plasmid#214076PurposeLuminescent reporter system to measure EJC-enhanced NMD activity. Intron present in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationLinker sequence present between firefly luciferas…PromoterAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKAd5f11p-EPG
Plasmid#122697PurposeTo generate E1/E3-deleted human adenovirus 5 (HAdV-5) bearing a chimeric fiber and an EF1a promoter-controlled transgene.DepositorInsertGFP
UseAdenoviralTagsExpressionMutationPromoterEF1aAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKAd5f11pABR-EPG
Plasmid#122699PurposeIt can be used to construct a recombinant virus carrying other transgene or an adenovector with modified fiber.DepositorInsertGFP
UseAdenoviralTagsExpressionMutationPromoterAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-ASAP3ER
Plasmid#195358PurposeASAP3ER under control of human synapsin promoterDepositorInsertASAP3ER
UseAAVTagsGFPExpressionMammalianMutationPromoterhuman synapsinAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKIF1C-mCherry-FRB
Plasmid#185982PurposeMammalian expression of human KIF1C with mCherry and FRB tags.DepositorInsertKIF1C (KIF1C Human)
UseTagsmCherry and FRBExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9-scFv
Plasmid#78900PurposeExpresses scFv-VP64 under actin promoter for SunTag CRISPRa in Drosophila cellsDepositorInsertscFV-VP64
UseCRISPRTagsGFPExpressionInsectMutationPromoterpActin (Drosophila)Available sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKIF1C_P176L-mCherry-FRB
Plasmid#185983PurposeMammalian expression of human KIF1C with P176L mutation and mCherry and FRB tags.DepositorInsertKIF1C (KIF1C Human)
UseTagsmCherry and FRBExpressionMammalianMutationP176L - mutation causing HSP (SPG58)PromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9-VPR
Plasmid#78898PurposeExpresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.DepositorInsertdCas9-VPR
UseCRISPRTagsExpressionInsectMutationPromoterpActin (Drosophila)Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLQ9834_sgTet: pHR-hU6-CasMINI sgRNA_#2 (sgTet); EF1a-Puro-T2A-BFP- WPRE
Plasmid#176273PurposeExpression of CasMINI sgRNA targeting TRE3G promoter in dCasMINI-mediated GFP activation assays.DepositorInsertCasMINI sgRNA (sgTet) and BFP
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMeCP2miRFP670 pc3942
Plasmid#167568PurposeThe miRFP670-gene is ligated c-terminally to the rat meCP2-gene.DepositorInsertMeCp2 (Mecp2 Rat)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKIF1C_R169W-mCherry-FRB
Plasmid#185984PurposeMammalian expression of human KIF1C with R169W mutation and mCherry and FRB tags.DepositorInsertKIF1C (KIF1C Human)
UseTagsmCherry and FRBExpressionMammalianMutationR169W - mutation causing HSP (SPG58) and silent m…PromoterAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
C1-mCitrine-FKBP-EZRabd(t567D)
Plasmid#155227PurposeAcute increase in MPA when used with FRB-FKBP dimerizaton system upon addition with RapamycinDepositorInsertmCitrine fused to FKBP and the actin BD of Ezrin (T567D), aa552-586 for use with FRB and rapamycin induced dimerization system
UseTagsmCitrineExpressionMammalianMutationChanged Thr 567 to Asp in original EzrinPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Sig10-Fc-IG
Plasmid#183722PurposeExpresses the fusion protein containing the extracellular part of human Siglec-10 and the Fc region of human IgG1, and GFPDepositorInsertSiglec-10
UseRetroviralTagsFc of human IgG1ExpressionMutationPromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-Casp2-shRNA
Plasmid#157926PurposeKnockdown of caspase-2DepositorInsertCasp2 shRNA (Casp2 Mouse)
UseLentiviral and RNAiTagsGFPExpressionMutationPromotermouse U6Available sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cas12f-GE ver4.0
Plasmid#176543PurposeExpresses Cas12f-GE in mammalian cellsDepositorInsertsCas12f-GE ver4.0
Cas12f-GE ver4.0
UseTagsExpressionMammalianMutationPromoterU6 and chicken β-actinAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cas12f-GE ver3.0
Plasmid#176508PurposeExpresses Cas12f-GE in mammalian cellsDepositorInsertsCas12f-GE ver3.0
Cas12f-GE ver3.0
UseTagsExpressionMammalianMutationPromoterU6 and chicken β-actinAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Sig10(M1)-Fc-IG
Plasmid#183723PurposeExpresses the fusion protein containing the extracellular part of the human Siglec-10 R47Q mutant and the Fc region of human IgG1, and GFPDepositorInsertSiglec-10
UseRetroviralTagsFc of human IgG1ExpressionMutationR47QPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Sig10(M2)-Fc-IG
Plasmid#183724PurposeExpresses the fusion protein containing the extracellular part of the human Siglec-10 A108V mutant and the Fc region of human IgG1, and GFPDepositorInsertSiglec-10
UseRetroviralTagsFc of human IgG1ExpressionMutationA108VPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only