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Flag Depdc5 pRK5

Plasmid

#46340

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Depdc5 (DEPDC5 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Differs from Depdc5 isoform 1 by 10 amino acids …

Promoter

Available since

July 16, 2013

Availability

Academic Institutions and Nonprofits only

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pQFlag-USP7 CS puroR

Plasmid

#46752

Purpose

Retroviral vector that expresses catalytically inactive form of Flag-tagged human USP7

Depositor

Gordon Peters, Goedele Maertens

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP7 (USP7 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

C223S--catalytically inactive

Promoter

CMV

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-HDAC4-FLAG

Plasmid

#30485

Depositor

Tso-Pang Yao

Insert

HDAC4 (HDAC4 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pTripZ RB1 WT-3XFLAG

Plasmid

#209070

Purpose

DOX-ON inducible expression vector that expresses RB1 WT with c-terminal 3X flag tag

Depositor

Matthew Oser

Article

Oser et al Cancer Discov. 2019 Feb;9(2):230-247. do

Insert

human RB1 WT after LR recombination with pTripZ NEO DEST to inducibly express RB1 WT with a c-terminal 3X flag tag

Use

Lentiviral

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pPIK16045_pX330_Flag-SuperFi-Cas9

Plasmid

#184370

Purpose

Mammalian expression of SuperFi-Cas9 nuclease

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

Insert

SuperFi-Cas9

Use

CRISPR

Tags

3X FLAG and NLS

Expression

Mammalian

Mutation

Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, K…

Promoter

CBh

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA Flag-Smad4M

Plasmid

#14959

Depositor

Joan Massague

Article

Kang et al Proc Natl Acad Sci U S A. 2005 Sep 27. 1

Insert

Smad4 (SMAD4 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

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Flag Nprl2 pRK5

Plasmid

#46333

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Nprl2 (NPRL2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pFLAG UBR1 SBX

Plasmid

#24506

Depositor

Alexander Varshavsky

Article

Xia et al J Biol Chem. 2008 Aug 29. 283(35):24011-

Insert

UBR1 (UBR1 Budding Yeast)

Use

Tags

FLAG

Expression

Yeast

Mutation

Promoter

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only
plenti-FLAG-CAMK2D-ED

Plasmid

#221700

Purpose

Lentiviral expression of FLAG-tagged human CAMK2D (K43R/D136N) in mammalian cells

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

CAMK2D (CAMK2D Human)

Use

Lentiviral

Tags

Expression

Mutation

K43R, D136N

Promoter

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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plenti-FLAG-CAMK2D

Plasmid

#221699

Purpose

Lentiviral expression of FLAG-tagged human CAMK2D in mammalian cells

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

CAMK2D (CAMK2D Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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CbS-Flag-TRRAP

Plasmid

#32103

Depositor

Michael Cole, Yardena Samuels

Insert

Transformation/transcription domain-associated protein (TRRAP Human)

Use

Tags

Flag 1x

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 14, 2011

Availability

Academic Institutions and Nonprofits only

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Flag-PML IV/pRK5

Plasmid

#59742

Purpose

Expresses the isoform IV of Promyelocytic Leukemia Protein (PML) in mammalian cells

Depositor

Xiaolu Yang

Article

Guo et al Mol Cell. 2014 Jul 3;55(1):15-30. doi: 1

Insert

PML isoform IV (PML Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA3-AUP1-FLAG

Plasmid

#185336

Purpose

Mammalian expression of AUP1

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Use

Tags

FLAG, 6xHis

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pBOBI C-FLAG PHGDH

Plasmid

#212995

Purpose

expresses human PHGDH

Depositor

Sheng-cai Lin

Article

Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

Insert

PHGDH (PHGDH Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

cmv

Available since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV5B-Flag-Smurf2 wt

Plasmid

#11746

Depositor

Jeff Wrana

Article

Kavsak et al Mol Cell. 2000 Dec . 6(6):1365-75.

Insert

SMURF2 (SMURF2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Blast-3xFLAG

Plasmid

#207684

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-3xFLAG cassette

Depositor

Laurence Pelletier

Article

Philip et al bioRxiv

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA-HDAC6-FLAG

Plasmid

#30482

Depositor

Tso-Pang Yao

Article

Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

Insert

HDAC6 (HDAC6 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pRK-flag-USP19

Plasmid

#78597

Purpose

Express flag-USP19 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 (USP19 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-mGCN2-FLAG-IP

Plasmid

#101794

Purpose

Retroviral vector encoding C-terminally FLAG tagged mouse GCN2

Depositor

Seiichi Oyadomari

Article

Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

Insert

Gcn2 (Eif2ak4 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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LPC-flag-REST-WT

Plasmid

#41903

Depositor

Stephen Elledge

Article

Westbrook et al Nature. 2008 Mar 20;452(7185):370-4. doi

Insert

RE1-silencing transcription factor (REST Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2013

Availability

Academic Institutions and Nonprofits only

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