Skip to main content
Addgene
Showing: 1201 - 1220 of 3108 results

  4. AAV Intein-C-SpyCas9, CMV-AcrIIA4-2xmiR-122 target sites

    Plasmid
    #120295
    Purpose
    AAV Vector for expression of C-terminal SpyCas9 fragement with split-intein and a CMV-driven AcrIIA4 with two miR-122 binding sites
    Depositor
    Dominik Niopek
    Article
    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545
    Insert
    C-terminal fragment of SpyCas9
    Use
    AAV and CRISPR
    Tags
    split-intein
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    120295-plasmid-map-sequence-id-232737

    Enlarge

  6. pcU6_3 MS2 sgRNA

    Plasmid
    #92394
    Purpose
    Chick-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter, with tracrRNA scaffold containing stem loops allowing binding of bacteriophage MS2 coat protein MCP
    Depositor
    Tatjana Sauka-Spengler
    Article
    Williams et al Development. 2018 Feb 23;145(4). pii: de
    Insert
    chick U6.3 promoter and gRNA cloning cassette including MS2 stem loops
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    chick U6.3
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    92394-plasmid-map-sequence-id-191866

    Enlarge

  7. pYPQ131-tRNA2.0

    Plasmid
    #158393
    Purpose
    Golden Gate entry vector to express the 1st gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Without promoter
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    158393-plasmid-map-sequence-id-330365

    Enlarge

  8. pYPQ134B2.0

    Plasmid
    #167158
    Purpose
    Golden Gate entry vector to express the 4th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    AtU3
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    167158-plasmid-map-sequence-id-331276

    Enlarge

  11. pYPQ135B2.0

    Plasmid
    #167159
    Purpose
    Golden Gate entry vector to express the 5th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    AtU3
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    167159-plasmid-map-sequence-id-331281

    Enlarge

  12. pYPQ136B2.0

    Plasmid
    #167160
    Purpose
    Golden Gate entry vector to express the 6th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    AtU3
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    167160-plasmid-map-sequence-id-331282

    Enlarge

  13. pYPQ132-tRNA2.0

    Plasmid
    #158394
    Purpose
    Golden Gate entry vector to express the 2nd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Without promoter
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    158394-plasmid-map-sequence-id-330366

    Enlarge

  15. pYPQ133-tRNA2.0

    Plasmid
    #158395
    Purpose
    Golden Gate entry vector to express the 3rd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Without promoter
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    158395-plasmid-map-sequence-id-330367

    Enlarge

  16. pYPQ134-tRNA2.0

    Plasmid
    #158396
    Purpose
    Golden Gate entry vector to express the 4th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Without promoter
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    158396-plasmid-map-sequence-id-330816

    Enlarge

  17. pYPQ135-tRNA2.0

    Plasmid
    #158397
    Purpose
    Golden Gate entry vector to express the 5th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Without promoter
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    158397-plasmid-map-sequence-id-330905

    Enlarge

  18. pYPQ136-tRNA2.0

    Plasmid
    #158398
    Purpose
    Golden Gate entry vector to express the 6th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Without promoter
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    158398-plasmid-map-sequence-id-330906

    Enlarge

  19. pPBO.ACT006

    Plasmid
    #182716
    Purpose
    Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region, with eGFP for cloning
    Depositor
    Joseph Schoeniger
    Article
    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666
    Insert
    CasRx crRNA cloning backbone
    Use
    CRISPR
    Tags
    GFP in cloning site of crRNA for easier screening…
    Expression
    Bacterial
    Mutation
    Catalytically deactivated R295A, H300A, R849A, H8…
    Promoter
    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)
    Available since
    Aug. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    182716-plasmid-map-sequence-id-361297

    Enlarge

  20. pRDA_792

    Plasmid
    #216084
    Purpose
    for adding plate and well barcodes via 6-9 and 9-10 modules, destination vector
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Insert
    for adding plate and well barcodes via 6-9 and 9-10 modules
    Use
    Lentiviral; Destination
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216084-plasmid-map-sequence-id-431431

    Enlarge

Showing: 1201 - 1220 of 3108 results