We narrowed to 22,746 results for: cas9

1. Lenti-eCas9-sgNT

   Plasmid

   #140238

   Purpose

   Lentiviral vector expressing high-specificity eSpCas9(1.1) and a control non-targeting sgRNA

   Depositor

   Urs Greber

   Article

   Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

   Insert

   non-targeting sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. eSpCas9(1.1)_No_FLAG_LMNA_G2

   Plasmid

   #178090

   Purpose

   Expresses the LMNA G2 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with LMNA_mScarlet-I_Donor to tag LMNA with mScarlet-I. pX330-like plasmid.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   LMNA G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Rad51–Cas9(D10A)

   Plasmid

   #125567

   Purpose

   Expresses the Rad51–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

   Depositor

   David Liu

   Article

   Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

   Insert

   Rad51–Cas9(D10A) (RAD51 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p-dCas9-VP64-Hygro

   Plasmid

   #104405

   Purpose

   transient expression of dCas9-VP64 fusion protein

   Depositor

   Stephan Beck

   Insert

   VP64
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available Since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pYPQ-dpcoCas9-Act3.0

   Plasmid

   #158408

   Purpose

   CRISPR-Act3.0 system containing dpcoCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Insert

   dpcoCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#2

   Plasmid

   #172984

   Purpose

   CRISPRi for TAOK2

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   sgTAOK2i (TAOK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. U6-CjCas9-MS2gRNA-Backbone

   Plasmid

   #210711

   Purpose

   This plasmid express MS2 loop containing Cj Cas9 gRNA

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   MS2 loop containing Cj Cas9 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. P823_eSpCas9(1.1)-RecA

   Plasmid

   #87264

   Purpose

   Human codon optimized RecA protein was fused to eSpCas9(1.1) for enhanced genome editing efficiency

   Depositor

   Yonglun Luo

   Article

   Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

   Insert

   RecA
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. eSpCas9(1.1)_No_FLAG

   Plasmid

   #79877

   Purpose

   FLAGless construct expressing high specificity eSpCas9(1.1). Px330-like plasmid.

   Depositor

   Yannick Doyon

   Article

   Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Untagged eSpCas9(1.1)

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. G1397 DddAtox-N–dSpCas9

    Plasmid

    #157835

    Purpose

    expresses split DddAtox-Cas9 construct in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    bpNLS–G1397 DddAtox-N–dSpCas9–UGI–UGI–bpNLS
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pX330-Flag-deSpCas9

    Plasmid

    #92114

    Purpose

    Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive eSpCas9 with FLAG tag
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A, K848A, K1003A, R1060A

    Promoter

    Cbh

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SpCas9 TadCBEa-V106W

    Plasmid

    #193838

    Purpose

    Express TadCBEa-V106W (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDa V106W-SpCas9 D10A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNS38-SadCas9-SNAP

    Plasmid

    #113718

    Purpose

    Bacterial vector for expression of Snap-tagged Staphylococcus aureus dCas9

    Depositor

    Gerald Schwank

    Article

    Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA-2xNLS-SNAP-NLS and His6-MBP-TEV

    Expression

    Bacterial

    Mutation

    D10A, N580A

    Promoter

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. xCas9(3.6)-BE4

    Plasmid

    #108386

    Purpose

    Mammalian expression vector for xCas9(3.6)-BE4

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9(3.6)-BE4
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

    Promoter

    Available Since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRS315e_pGal-dCas9-PmCDA1

    Plasmid

    #79615

    Purpose

    Expresses dCas9-PmCDA1 in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Yeast

    Mutation

    D10A and H840A for nuclease deficient Cas9

    Promoter

    pGal1

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. (364) SB KRAB-SadCas9

    Plasmid

    #163022

    Purpose

    Sleeping Beauty TET-On expression of CRISPRi (without U6-gRNA)

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Mammalian codon-optimized SadCas9
    

    Use

    Transposon

    Tags

    KRAB and myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TET-On

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-Cas9-NRRH

    Plasmid

    #136924

    Purpose

    Expresses Cas9-NRRH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    Cas9-NRRH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti6.3-SauriCas9-BlastR

    Plasmid

    #220967

    Purpose

    Expresses human codon-optimized SauriCas9

    Depositor

    Hyongbum Kim

    Article

    Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

    Insert

    SauriCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid

    #112922

    Purpose

    Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidine resistant marker

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAV-P3-evoCjCas9-eAID_gRNA

    Plasmid

    #202562

    Purpose

    P3-evoCjCas9-eAID and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9-eAID
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge