Showing: 1201 - 1220 of 1585 results
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Plasmid#49258PurposeExpresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.DepositorInsertcaspase-2 (CASP2 Human)
UseTagsflag and split Venus NExpressionMammalianMutationC303SPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pBiFC-HA-hCaspase 2 CARD-VC155
Plasmid#49259PurposeExpresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.DepositorInsertCaspase-2 CARD (CASP2 Human)
UseTagsHA and split Venus CExpressionMammalianMutationdeleted aa 123-435PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pBiFC-flag-hCaspase 2 CARD-VN173
Plasmid#49260PurposeExpresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.DepositorInsertcaspase-2 CARD (CASP2 Human)
UseTagsFlag and split Venus NExpressionMammalianMutationdelete aa 123-435PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pBiFC-HA-hCaspase 2 prodomain-VC155
Plasmid#49261PurposeExpresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.DepositorInsertcaspase-2 prodomain (CASP2 Human)
UseTagsHA and split Venus CExpressionMammalianMutationdelete aa 148-435PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pBIFC-flag-hCaspase 2 prodomain-VN173
Plasmid#49262PurposeExpresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.DepositorInsertcaspase-2 prodomain (CASP2 Human)
UseTagsflag and split Venus NExpressionMammalianMutationdelete aa 148-435PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pBiFC-PBZ-VN
Plasmid#110646Purposeexpresses a PAR-binding (PBZ) domain fused to venus N-terminusDepositorInsertPBZ domain from APLF, amino acids 371-451 (APLF Human)
UseTagsDDK and VNExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pBiFC-PBZ-VC
Plasmid#110648Purposeexpresses a PAR-binding (PBZ) domain fused to venus C-terminusDepositorInsertPBZ domain from APLF, amino acids 371-451 (APLF Human)
UseTagsHA and VCExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pKK-mAmber-TEV
Plasmid#105775PurposeExpression of your protein of interest in fusion with non-absorbing, non-emitting Venus mutant at the N-terminus (cleavable by TEV). Negative control for FRET measurements (PMID: 17040988, 22264545).DepositorTypeEmpty backboneUseFlp-in competentTagsmAmber-TEVExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-mAmber
Plasmid#105792PurposeExpression of your protein of interest in fusion with non-absorbing, non-emitting Venus mutant at the C-terminus (cleavable by TEV). Negative control for FRET measurements (PMID: 17040988, 22264545).DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mAmberExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
KA1042_pPB-hCMV*1-Esrrb-IV
Plasmid#124177PurposepiggyBac-based Dox-inducible expression of EsrrbDepositorInsertEsrrb-IRES-Venus (Esrrb Mouse)
UseTagsExpressionMammalianMutationPromotertetO-CMVminAvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-H2-Ld-VN
Plasmid#135520PurposeMammalian expression of VN-fused and myc-tagged H2-LdDepositorInsertH2-Ld
UseTagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Raichu-OsRac1 WT
Plasmid#133262PurposeBiosensor for monitoring activation for rice small GTPase OsRac1DepositorInsertOsRac1 (LOC4325879 Oryza sativa)
UseTagsCFP-lipid and Venus-CRIBExpressionPlantMutationPromoterUbiAvailabilityAcademic Institutions and Nonprofits only -
Raichu-OsRac1 DN
Plasmid#133263PurposeDominant-negative (DN) mutant of OsRac1; Acts as a negative control for small GTPases activation assaysDepositorInsertOsRac1(T24N) (LOC4325879 Oryza sativa)
UseTagsCFP-lipid and Venus-CRIBExpressionPlantMutationOsRac1 T24NPromoterUbiAvailabilityAcademic Institutions and Nonprofits only -
Raichu-OsRac1 CA
Plasmid#133264PurposeConstitutively-active (CA) mutant of OsRac1; Acts as a positive control for small GTPases activation assays.DepositorInsertOsRac1(G19V) (LOC4325879 Oryza sativa)
UseTagsCFP-lipid and Venus-CRIBExpressionPlantMutationOsRac1 G19VPromoterUbiAvailabilityAcademic Institutions and Nonprofits only -
LIC-Z-YFP
Plasmid#153544Purposeperform light induced optogenetic clustering of TCR ζ-Chain tagged with yellow fluorescent protein as described in the associated publicationDepositorInserthuman TCR ζ-Chain (CD247 Human)
UseTagsVenus YFPExpressionMammalianMutationwith mCherry in LIC-Z replaced by YFPPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
G12-CASE
Plasmid#190714PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein G12. Composed of the subunits G alpha 12 (GNA12) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorInsertsGNB3
GNGT9
GNA12
UseLuciferaseTagsNluc is inserted at A133/F134 within GNA12 and cp…ExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJW1507
Plasmid#62361PurposepFA6a -mVenus-BirA::HIS5DepositorInsertc-terminal mVenus-BirA tagging
UseYeast c-terminal tagging vectorTagsmVenus-BirAExpressionMutationL232H mutation compared to standard mVenusPromoterAvailabilityAcademic Institutions and Nonprofits only -
pVC
Plasmid#175063Purposeexpresses mVenus and mCherry pair in mammalian cellsDepositorInsertmVenus + mCherry
UseTagsExpressionMammalianMutationmVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…PromoterCMV PromoterAvailabilityAcademic Institutions and Nonprofits only -
pLBM0090
Plasmid#172824PurposeLevel 2 module containing mCHERRY-nVENUS HSP21-cVENUSDepositorInsert[35S-P19][35S-CTPSSU-mHSP21-nVENUS][35S-CTPSSU-mHSP21-X3FLAGcVENUS][35S-SP_SV40-CFP]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pQ54-VC
Plasmid#175060Purposeexpresses mVenus and mCherry tagged to a mutant of the huntingtin exon1 (polyQ54) in mammalian cellsDepositorInsertpolyQ54 (HTT Human)
UseTagsmVenus + mCherryExpressionMammalianMutationmVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…PromoterCMV PromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 1201 - 1220 of 1585 results