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Plasmid#180484PurposepFastBac1 human CENP-E-mNeon-His, containing a 3c protease cleavage site between mNeon and His tag. Codon optimized for expression in Sf9 cellsDepositorInsertCENP-E (CENPE Human, Synthetic, human)
UseTagsmNeon-6xHisExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT mCh-V5-NES-EGFP-EGFR NLS
Plasmid#192315PurposeGateway entry vector for an inducible mCh-V5-NES-EGFP-EGFR NLSDepositorInsertmCh-V5-NES-EGFP-EGFR NLS (EGFR Human)
UseGateway entry vectorTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT mCh-V5-NES-EGFP-Erbb2 NLS
Plasmid#192317PurposeGateway entry vector for an inducible mCh-V5-NES-EGFP-Erbb2 NLSDepositorInsertmCh-V5-NES-EGFP-Erbb2 NLS (Erbb2 Rat)
UseGateway entry vectorTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOmniBac zz TEV YBBR TRF1
Plasmid#185445PurposeExpresses human TRF1 with a zz affinity tag, YBBR labeling site, and TEV cleavage site in insect cellsDepositorInsertTRF1 (TERF1 Human)
UseTagsYBBR and ZZExpressionInsectMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-gamma-tubulin(N229A)-TEV-His6
Plasmid#178077PurposeInsect cell expression of gamma-tubulin(N229A)-TEV-His6DepositorInsertgamma-tubulin with an N229A point mutation (TUBG1 Human)
UseTagsTEV-His6ExpressionInsectMutationChanged Asparagine 229 to AlaninePromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR207-MUM2sp-Citrine-ADPG2
Plasmid#170725PurposeGateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the Citrine and the coding sequence of the HG degrading enzyme ADPG2 (At2g41850.1)DepositorInsertARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2 (PGAZAT Mustard Weed)
UseGateway donor vector / entry cloneTagsCitrine tag (714bp)ExpressionMutationPromoterAvailable sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR207-MUM2sp-ADPG2
Plasmid#170723PurposeGateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the HG degrading enzyme ADPG2 (At2g41850.1)DepositorInsertARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2 (PGAZAT Mustard Weed)
UseGateway donor vector / entry cloneTagsExpressionMutationPromoterAvailable sinceNov. 11, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBac1_GI.1_A37C-A44C
Plasmid#162582PurposeExpresses norovirus GI.1 VP1 protein with mutations A37C-A44C in Sf9 insect cellsDepositorInsertVP1 protein from human norovirus GI.1
UseTagsExpressionInsectMutationAlanine 37 changed to Cysteine and Alanine 44 cha…PromoterpolyhedringAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1_GI.1_Q141V-P221L
Plasmid#162584PurposeExpresses norovirus GI.1 VP1 protein with mutations Q141V-P221L in Sf9 insect cellsDepositorInsertVP1 protein from human norovirus GI.1
UseTagsExpressionInsectMutationGlutamine 141 changed to Valine and Proline 221 c…PromoterpolyhedrinAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1_GI.1_L144C-P221C
Plasmid#162585PurposeExpresses norovirus GI.1 VP1 protein with mutations L144C-P221C in Sf9 insect cellsDepositorInsertVP1 protein from human norovirus GI.1
UseTagsExpressionInsectMutationLeucine 144 changed to Cysteine and Proline 221 c…PromoterpolyhderinAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1_GI.1_G131C-N172C
Plasmid#162586PurposeExpresses norovirus GI.1 VP1 protein with mutations G131C-N172C in Sf9 insect cellsDepositorInsertVP1 protein from human norovirus GI.1
UseTagsExpressionInsectMutationGlycine 131 changed to Cysteine and Asparagine 17…PromoterpolyhedrinAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1_GI.1_N167C-L169C
Plasmid#162587PurposeExpresses norovirus GI.1 VP1 protein with mutations N167C-L169C in Sf9 insect cellsDepositorInsertVP1 protein from human norovirus GI.1
UseTagsExpressionInsectMutationAsparagine 167 changed to Cysteine and Leucine 16…PromoterpolyhedrinAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-MAVS-Gln93
Plasmid#158629PurposeGateway entry vector for an inducible 3xFLAG-tagged MAVS mutantDepositorInsertMAVS (MAVS Human)
UseGateway entry vectorTags3XFLAGExpressionMutationE93QPromoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-MAVS-Ala148
Plasmid#158630PurposeGateway entry vector for an inducible 3xFLAG-tagged MAVS mutantDepositorInsertMAVS (MAVS Human)
UseGateway entry vectorTags3XFLAGExpressionMutationQ148APromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)
Plasmid#79030PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5alpha(African green monkey pygerythrus)(1-293)
UseTagsOneSTrEP-FLAGExpressionInsectMutationdeleted amino acid 294-515 from frican green monk…PromoterpolyhedrinAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pinducer 20 DN-KASHΔPPPL
Plasmid#129280PurposeTet-ON inducible lentivirus expressing mcherry-labeled DN KASHΔPPPLDepositorInsertSYNE1 (SYNE1 Human)
UseLentiviralTagsmcherryExpressionMutationthe last 4 amino acids (PPPL) of the KASH domain …PromoterCMVAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-Mb-sfGFP WT
Plasmid#197569PurposeExpression of sfGFP WT with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cellsDepositorInsertssfGFP WT
Pyl-tRNA (4 copies)
UseTagsV5-His6ExpressionMammalianMutationPromoterCMV and U6/H1Available sinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDyn1
Plasmid#64067PurposeHuman cytoplasmic dynein heavy chain (DYNC1H1) codon optimized for Sf9 expression with a SNAPf tag for fluorescent labeling and a TEV-cleavable His-ZZ tag for purification (His-ZZ-TCS-SNAPf-DYNC1H1).DepositorInsertDYNC1H1 (DYNC1H1 Human)
UseCre/LoxTagsHis-ZZ-TCS-SNAPfExpressionInsectMutationCodon optimized for Sf9PromoterAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.dEZH2
Plasmid#220241PurposeExpression of an EZH2 catalytic site mutant in insect cells.DepositorInsertdEZH2 (EZH2 Human)
UseTags6x His, MBPExpressionInsectMutationY731FPromoterPolyhedrinAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-Mb-Fluoro-Phe D6
Plasmid#197577PurposeExpression of Methanosarcina barkeri (Mb) Fluoro-Phe D6 tRNA synthetase/Pyl-tRNA pair for the encoding of fluorinated phenylalanine derivatives at TAG codons in HEK293 cellsDepositorInsertsMb Pyl Fluoro-Phe B5 synthetase
Pyl-tRNA (4 copies)
UseTagsNuclear export sequence + FLAGExpressionMammalianMutationN311A, C313M, V366G, W382TPromoterCMV and U6/H1Available sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only