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Plasmid#24262DepositorInsertBmf (S87A,S90A) (BMF Human)
UseTagsFlagExpressionMammalianMutationchanged Serine 74 to Alanine changed Serine 77 t…PromoterAvailable sinceJune 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag Bmf (S74D,S77D)
Plasmid#24263DepositorInsertBmf (S87D,S90D) (BMF Human)
UseTagsFlagExpressionMammalianMutationchanged Serine 74 to Aspartic Acid changed Serin…PromoterAvailable sinceJune 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide Puro-P2A-EGFP
Plasmid#137729PurposeFor simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).DepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyTagsEGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-SUMO-hGSDMD
Plasmid#111559PurposeExpress human GSDMD in E.coliDepositorInsertGasdermin D (GSDMD Human)
UseTagsHis, SUMOExpressionBacterialMutationPromoterT7Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
CLXSN 5myc-CDC6-WT
Plasmid#109332Purposeexpress tagged Cdc6 WTDepositorInsertCDC6 (CDC6 Human)
UseRetroviralTags5x MycExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
hCathepsin B
Plasmid#11249PurposeMammalian expression of human cathepsin BDepositorInsertcathepsin B (CTSB Human)
UseTagsC9 (GTETSQVAPA)ExpressionMammalianMutationPromoterAvailable sinceMay 25, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP-2xFYVE
Plasmid#140047PurposeExpresses GFP-tagged HRS 2xFYVE in mammalian cellsDepositorInserttandem HRS FYVE domain (Hgs Mouse)
UseTagsEGFPExpressionMammalianMutationtwo FYVE domains (amino acids 147-223), linked by…PromoterCMVAvailable sinceMay 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA GNSTM-3-Flag-10-Lamp2b-HA
Plasmid#71293PurposeEncodes (N to C): GNSTM glycosylation motif, 3 residue spacer, Flag tag,10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.DepositorInsertLamp2b (LAMP2 Human)
UseTagsFLAG, GNSTM glycosylation motif, HA, and Lamp2 si…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDB2-hPER1-mScarlet-I-CD4-bla
Plasmid#189980PurposeDonor Vector to integrate mScarlet-I C-terminal into the human PER1 Locus to express it as a fusion proteinDepositorInsertmScarlet-I
UseCRISPRTags3xFlagExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCriprV2-sgRNA-PER1-#1
Plasmid#189987PurposeLentiviral Cas9/sgRNA vector targeting C-terminus of hPER1, works with Addgene 189979-189982DepositorInsertPeriod1 (PER1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEMS1943
Plasmid#82565PurposeHigh copy number plasmid containing Ple266 (S100B MiniPromoter) insert.DepositorInsertPle266
UsePleiades promoter project [sic, pleaides plieades]TagsExpressionMutationPromoterAvailable sinceSept. 14, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pClneoMyc human ERBIN
Plasmid#40214DepositorInsertERBIN (ERBIN Human)
UseTagsmycExpressionMammalianMutationL143S, S274L and P338L mutation in ERBIN compared…PromoterCMVAvailable sinceSept. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
STR-KDEL_PGRN-SBP-mCherry
Plasmid#180439PurposeCo-expresses (via IRES) streptavidin-KDEL and Progranulin-SBP-mCherryDepositorInsertProgranulin (GRN Human)
UseTagsSBP-mCherryExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNAZeo(-) LAMP2C
Plasmid#89342PurposeExpresses human LAMP-2C in mammalian cellsDepositorInsertLysosomal-associated membrane protein 2C (LAMP2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
PT3.5/CMV-EGFRvIII
Plasmid#20280DepositorInsertEGFRvII (EGFR Human)
UseTransposonTagsExpressionMutationSee depositor comments below.PromoterAvailable sinceDec. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCAG/ATP8B1-HA
Plasmid#209209PurposeMammalian expression of ATP8B1DepositorInsertATP8B1 (ATP8B1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG/ATP8A1-HA
Plasmid#209208PurposeMammalian expression of ATP8A1DepositorInsertATP8A1 (ATP8A1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIG-VP16–RAR-alpha
Plasmid#80434PurposeExpression of constitutively active human RAR-alpha in chick embryosDepositorInsertRetinoic acid receptor alpha (RARA Human)
UseTagsEGFP and VP16ExpressionMammalianMutationPromoterbeta-actin promoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
p420-3
Plasmid#71257Purposeluciferase reporter with 3 copies of the GM420 NFAT-AP-1 site in front of hGM-CSF -55 to +28 minimal promoterDepositorInsert3 copies of the GM420 NFAT-AP-1 site (CSF2 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterGM-CSF minimalAvailable sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G_NT-GSDMD
Plasmid#201170PurposeDox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transductionDepositorInsertGasdermin D N-terminal domain (Gsdmd Mouse)
UseRetroviralTagsExpressionMutationaa1-276 onlyPromoterTRE3GAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only