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Plasmid#210132Purposeknock out BAK in mammalian cellsDepositorInsertBcl-2 homologous antagonist/killer (BAK1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgBAK-1
Plasmid#210131Purposeknock out BAK in mammalian cellsDepositorInsertBcl-2 homologous antagonist/killer (BAK1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgBAX-1
Plasmid#210013Purposeknock out BAX in mammalian cellsDepositorInsertApoptosis regulator BAX (BAX Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP C1-Eps8 ∆cap
Plasmid#74786Purposemammalian expression of the capping activity mutant Eps8 fused to GFPDepositorInsertEps8 (Eps8 Mouse)
UseTagsExpressionMammalianMutationV689D, L693DPromoterAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP C1-Eps8 ∆bund
Plasmid#74889Purposemammalian expression of the bundling activity mutant Eps8 fused to GFPDepositorInsertEps8 (Eps8 Mouse)
UseTagsExpressionMammalianMutationL757A, K759APromoterAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP C1-Eps8 ∆cap/∆bund
Plasmid#74891Purposemammalian expression of the capping and bundling activity mutant Eps8 fused to GFPDepositorInsertEps8 (Eps8 Mouse)
UseTagsEGFPExpressionMammalianMutationV689D, L693D, L757A, K759APromoterAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_delta5C-PolyA
Plasmid#112289PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) delta5C mutant lacking the last five residues (FLTWL) in the PDZ binding domain at the N-terminus of the proteinDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationHuman YAP1-2gamma lacking the last 5 Amino acids …PromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_Y3F-PolyA
Plasmid#112288PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) Y3F mutant _ tyrosines 341, 357 and 394 corresponding to tyrosines 391, 407 and 444 in this isoformDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutation3 Tyrosines (341, 357 and 394 corresponding to ty…PromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 mCherry_K-RasC14
Plasmid#101286PurposeLentiviral expression of K-Ras C14DepositorInsertK-Ras (KRAS Human)
UseLentiviralTagsmCherryExpressionMammalianMutationC14PromoterAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgABI2-1
Plasmid#210139Purposeknock out ABI2 in mammalian cellsDepositorInsertAbl interactor 2 (ABI2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgCYFIP1-1
Plasmid#210137Purposeknock out CYFIP1 in mammalian cellsDepositorInsertCytoplasmic FMR1-interacting protein 1 (CYFIP1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgABI2-2
Plasmid#210140Purposeknock out ABI2 in mammalian cellsDepositorInsertAbl interactor 2 (ABI2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgHSPC300-1
Plasmid#210141Purposeknock out HSPC300 in mammalian cellsDepositorInsertProtein BRICK1 (BRK1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgHSPC300-2
Plasmid#210142Purposeknock out HSPC300 in mammalian cellsDepositorInsertProtein BRICK1 (BRK1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgCYFIP1-2
Plasmid#210138Purposeknock out CYFIP1 in mammalian cellsDepositorInsertCytoplasmic FMR1-interacting protein 1 (CYFIP1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHD-dsRed-attP_dCrk_flankingHRs
Plasmid#131141PurposeContains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger).DepositorUseCRISPR and Cre/Lox; Homology directed repair temp…TagsExpressionMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
E. coli BL21 (DE3) ΔEntD
Bacterial Strain#192874PurposeDeletion of native E. coli EntD, a 4'-PPTase required for native enterobactin synthesis. Allows expression of NRPS carrier proteins that do not harbor a phosphopantetheinyl group.DepositorBacterial ResistanceNoneSpeciesEscherichia coliAvailable sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-ARP3-N-12
Plasmid#53995PurposeLocalization: Actin Related Protein, Excitation: 487, Emission: 509DepositorInsertARP3 (ACTR3 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mEmerald-Espin-C
Plasmid#54084PurposeLocalization: Actin Bundling , Excitation: 487, Emission: 509DepositorInsertEspin (ESPN Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 2, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -