Addgene

We narrowed to 3,036 results for: aaas

Showing: 1181 - 1200 of 3036 results

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mCherry-FKBP-SAC1-HLx6

Plasmid

#108135

Purpose

Recruitable SAC1 with extended linker

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-520):[EAAAR]6:SACM1L(521-587) (SACM1L Human)

Use

Tags

Expression

Mammalian

Mutation

SACM1L(1-520):[EAAAR]6:SACM1L(521-587)

Promoter

CMV

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-SAC1-HLx4

Plasmid

#108134

Purpose

Recruitable SAC1 with extended linker

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG::SACM1L(1-520):[EAAAR]4:SACM1L(521-587) (SACM1L Human)

Use

Tags

Expression

Mammalian

Mutation

SACM1L(1-520):[EAAAR]4:SACM1L(521-587)

Promoter

CMV

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-SAC1-HLx2

Plasmid

#108133

Purpose

Recruitable SAC1 with extended linker

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG::SACM1L(1-520):[EAAAR]2:SACM1L(521-587) (SACM1L Human)

Use

Tags

Expression

Mammalian

Mutation

SACM1L(1-520):[EAAAR]2:SACM1L(521-587)

Promoter

CMV

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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UNG D11.3 gRNA

Plasmid

#90936

Purpose

3rd generation lentiviral gRNA plasmid targeting human UNG

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

UNG (Guide Designation D11.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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PTTG1 A9.4 gRNA

Plasmid

#90862

Purpose

3rd generation lentiviral gRNA plasmid targeting human PTTG1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PTTG1 (Guide Designation A9.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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POC1A F11.2 gRNA

Plasmid

#90838

Purpose

3rd generation lentiviral gRNA plasmid targeting human POC1A

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

POC1A (Guide Designation F11.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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MCM10 B10.6 gRNA

Plasmid

#90757

Purpose

3rd generation lentiviral gRNA plasmid targeting human MCM10

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MCM10 (Guide Designation B10.6)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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SPAG5 G7.2 gRNA

Plasmid

#90900

Purpose

3rd generation lentiviral gRNA plasmid targeting human SPAG5

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SPAG5 (Guide Designation G7.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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CFDP1 C12.4 gRNA

Plasmid

#90625

Purpose

3rd generation lentiviral gRNA plasmid targeting human CFDP1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CFDP1 (Guide Designation C12.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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PAFAH1B1 G10.3 gRNA

Plasmid

#90823

Purpose

3rd generation lentiviral gRNA plasmid targeting human PAFAH1B1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PAFAH1B1 (Guide Designation G10.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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ECT2 B2.4 gRNA

Plasmid

#90675

Purpose

3rd generation lentiviral gRNA plasmid targeting human ECT2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ECT2 (Guide Designation B2.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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ZW10 H9.3 gRNA

Plasmid

#90942

Purpose

3rd generation lentiviral gRNA plasmid targeting human ZW10

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ZW10 (Guide Designation H9.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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TOPBP1 G1.4 gRNA

Plasmid

#90916

Purpose

3rd generation lentiviral gRNA plasmid targeting human TOPBP1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

TOPBP1 (Guide Designation G1.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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PTTG1 G9.1 gRNA

Plasmid

#90860

Purpose

3rd generation lentiviral gRNA plasmid targeting human PTTG1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PTTG1 (Guide Designation G9.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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DSN1 C12.1 gRNA

Plasmid

#90657

Purpose

3rd generation lentiviral gRNA plasmid targeting human DSN1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DSN1 (Guide Designation C12.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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Chr8q_Centromere-Targeting_gRNA

Plasmid

#195128

Purpose

gRNA targeting centromere-proximal location on Chromosome 8q in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Chr8q gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-NBRE3

Plasmid

#208254

Purpose

pGL3 promoter luciferase reporter vector containing 3 copies of the NBRE DNA response element (5′-GAGTTTTAAAAGGTCATGCTCAATT TGTC-3′) used for luciferase reporter assays in mammalian cells

Depositor

Douglas Kojetin

Article

Munoz-Tello et al J Med Chem. 2020 Dec 24;63(24):15639-156

Insert

3X NBRE-LUC

Use

Tags

Expression

Mammalian

Mutation

Promoter

SV40 early promoter

Available since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ7558 pHR (hU6-crB2M-EFS-PuroR-WPRE)

Plasmid

#214881

Purpose

Lentiviral vector encoding RfxCas13d targeting B2M guide

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crB2M-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shMKP5.v2 puro

Plasmid

#87793

Purpose

Expresses an inducible short hairpin targeting human MKP5 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP5 (DUSP10 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE tight

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shMKP5.v1 puro

Plasmid

#87792

Purpose

Expresses an inducible short hairpin targeting human MKP5 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP5 (DUSP10 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE tight

Available since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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