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Addgene
Showing: 1181 - 1200 of 1885 results

  5. 3xFLAG-dCas9/pMXs-puro

    Plasmid
    #51240
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Availability
    Academic Institutions and Nonprofits only
    51240-plasmid-map-sequence-id-410075

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  6. 3xFLAG-dCas9/pMXs-IG

    Plasmid
    #51258
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Availability
    Academic Institutions and Nonprofits only
    51258-plasmid-map-sequence-id-77869

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  7. 3xFLAG-dCas9/pMXs-neo

    Plasmid
    #51260
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Availability
    Academic Institutions and Nonprofits only
    51260-plasmid-map-sequence-id-77871

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  9. SEMA4-bio-His

    Plasmid
    #51827
    Purpose
    Expresses full-length Semaphorin-4D precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    SEMA4 (SEMA4D Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    51827-plasmid-map-sequence-id-79664

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  18. TetO-FUW-VdC9BV

    Plasmid
    #62195
    Purpose
    Expresses RNA-Guided, Nuclease-Inactive VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation of Endogenous Genes
    Depositor
    Kam Leong
    Article
    Chakraborty et al Stem Cell Reports. 2014 Dec 9;3(6):940-7
    Insert
    VP64dCas9BFPVP64
    Use
    Lentiviral
    Tags
    Two VP64s tagged to dCas9 fused to BFP
    Expression
    Mammalian
    Mutation
    D10A H840A (catalytically inactive) Cas9 (dCas9)
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    62195-plasmid-map-sequence-id-101643

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Showing: 1181 - 1200 of 1885 results