Addgene

We narrowed to 2,594 results for: BFP

Showing: 1181 - 1200 of 2594 results

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iF57 PB-FrtStopFrt-Zim3-dCas9-mScarlet-puro-BFP

Plasmid

#204727

Purpose

Frt-inducible CRISPRi by Zim3-dCas9

Depositor

Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

Insert

Zim3-dCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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iE65 PB-Zim3-XTEN-dCas9-mApple-puro-BFP

Plasmid

#204724

Purpose

Constitutive CRISPRi by Zim3-dCas9

Depositor

Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

Insert

Zim3-dCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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iE61 PB-Zim3-XTEN-dCas9-mScarlet-puro-BFP

Plasmid

#204722

Purpose

Constitutive CRISPRi by Zim3-dCas9

Depositor

Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

Insert

Zim3-dCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCA20-HSPB1 5'HA-mTagBFP-3'-UTR 12XMS2V5-3'HA (HDR donor)

Plasmid

#199451

Purpose

CRISPR donor plasmid to insert [mTagBFP-stop-12XMS2] into the C-terminus of human HSPB1 gene; 12XMS2 locates in the 3' UTR region

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

HDR donor template

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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EK0425 CMV-TO-BFPmut-stop-Bdnf.t+54@202 (FLP-IN)

Plasmid

#191147

Purpose

Expression of the sequence matching the 54 extra bases in EK0362 in mammalian cells

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

Bdfn.t+54

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TagBFP-P2A-Pre-SPHERE-Rncp-iGluSnFR1

Plasmid

#187093

Purpose

Glutamate sensor for localization to membrane contatct

Depositor

Kotaro Oka

Article

Shindo et al bioRxiv 2022

Insert

Pre-SPHERE-Rncp-iGluSnFR1

Use

Tags

TagBFP-P2A

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pHIE061 ZF43x6-C mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138742

Purpose

Integration Vector, assembled with pHIE045 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Compact

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pHIE065 ZF43x12-S mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138746

Purpose

Integration Vector, assembled with pHIE049 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x12-Spaced

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ1972 - [1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)

Plasmid

#159844

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pHIE059 ZF43x1 mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138740

Purpose

Integration Vector, assembled with pHIE043 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x1

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pHIE060 ZF43x3-C mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138741

Purpose

Integration Vector, assembled with pHIE044 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x3-Compact

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pHIE063 ZF43x3-S mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138744

Purpose

Integration Vector, assembled with pHIE047 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x3-Spaced

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIE064 ZF43x6-S mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138745

Purpose

Integration Vector, assembled with pHIE048 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Spaced

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIE062 ZF43x12-C mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138743

Purpose

Integration Vector, assembled with pHIE046 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x12-Compact

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHBS1506 [IBB-GFP-mCherry3E]-[BFP-TDP43 FYW-L]

Plasmid

#133330

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

All FYW to L in CTD of full-length TDP43

Promoter

Available since

Nov. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pHBS1507 [IBB-GFP-mCherry 3E]-[BFP-TDP43 VLIM-S]

Plasmid

#133331

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

All VLIM to S in CTD of full-length TDP43

Promoter

Available since

Nov. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pHBS1508 [IBB-GFP-mCherry 3E]-[BFP-TDP43 VLIM-F]

Plasmid

#133332

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

All VLIM to F in CTD of full-length TDP43

Promoter

Available since

Nov. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pHBS1401 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 F-S]

Plasmid

#118808

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

All F to S in CTD of full-length TDP43

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHBS1187 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 N-Q]

Plasmid

#107847

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

All asparagine to glutamine in splicing reporter

Promoter

Available since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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pHBS1185 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 F-S]

Plasmid

#107843

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

All phenylalanine to serine in splicing reporter

Promoter

Available since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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