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Plasmid#16447DepositorInsertp53-binding site (mutant) (TP53 )
UseBeta-gal reporterTagsbeta-galExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFastBac StrepII msfGFP TEV cloning vector with BioBrick polycistronic restriction sites(11R-GFP)
Plasmid#48297DepositorTypeEmpty backboneUseTagsStrepII and msfGFPExpressionInsectMutationPromoterAvailable sinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V3)
Plasmid#55217DepositorTypeEmpty backboneUseTagsSNAPf and TwinStrepIIExpressionInsectMutationPromoterAvailable sinceJuly 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V1)
Plasmid#55216DepositorTypeEmpty backboneUseTagsSNAPf and TwinStrepIIExpressionInsectMutationPromoterAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter TCF (0)-(4) sites mutant pGL3Basic
Plasmid#32737DepositorInsertCCND1 promoter (CCND1 Human)
UseLuciferaseTagsExpressionMutation-539 TCF(0) ATGAAAG deletion; -75 TCF(1) and -68 …Promoter-962 CCND1Available sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter TCF (1)-(4) sites mutant pGL3Basic
Plasmid#32736DepositorInsertCCND1 Promoter (CCND1 Human)
UseLuciferaseTagsExpressionMutation-75 TCF(1) and -68 TCF(2) CTTTGATCTTTGCT deletion…Promoter-962 CCND1Available sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter TCF (1)(2) sites mutant pGL3Basic
Plasmid#32734DepositorInsertCCND1 promoter (CCND1 Human)
UseLuciferaseTagsExpressionMutation-75 TCF(1) and -68 TCF(2) - CTTTGATCTTTGCT deleti…Promoter-962 CCND1Available sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter TCF (1)-(3) sites mutant pGL3Basic
Plasmid#32735DepositorInsertCCND1 Promoter (CCND1 Human)
UseLuciferaseTagsExpressionMutation-75 TCF(1) and -68 TCF(2) CTTTGATCTTTGCT deletion…Promoter-962 CCND1Available sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pXWJ109As(RS-te-AAV)
Plasmid#123156PurposeFor TEV-based leakiness regulation. pSB3K3 carrying J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-te-AAV-tDepositorInsertJ109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-te-AAV-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ109, ParsR, PhrpLEAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tom20-mChF-AIP(TE)
Plasmid#61522PurposeExpression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIP with TE mutationDepositorInsertFK506 binding protein 1A (FKBP1A Human)
UseTagsAIP with TE mutation (see comments), Tom20, and m…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-His-TEV-OTC_d1_Ub
Plasmid#170729PurposeScaffold design for studying ubiquitin interacting proteins by cryo-EM.DepositorInsertE. coli ornithine transcarbamylase (OTC), fused to human ubiquitin
UseTags6xHis-TEVExpressionBacterialMutationThe C-terminus of OTC was truncated by removing o…PromoterT7Available sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-His-TEV-OTC_Ub
Plasmid#172134PurposeScaffold design for studying ubiquitin interacting proteins by cryo-EM.DepositorInsertE. coli ornithine transcarbamylase (OTC), fused to human ubiquitin
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
PGL4-4X TE Down (GQs) Renilla
Plasmid#70039PurposePlasmid for testing 5’UTR TE Down (G-quadruplex) folding elementsDepositorInsert4x TE Down (G-quadruplexes) in 5'UTR
UseLuciferaseTagsrenilla luciferaseExpressionMammalianMutation4 repeats of TE down motif (G-quadruplexes) in 5&…PromoterSV40Available sinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
EcNGRfEfC-fadD-tesA'
Bacterial Strain#48938PurposeBase line strain is heat shock induce-able lambda red recombination system for MAGE (Multiplex Recombination Genome Engineering)DepositorBacterial ResistanceSpectinomycinAvailable sinceDec. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
EcNGRfEfC-fadD-tesA'-fadR
Bacterial Strain#48939PurposeBase line strain is heat shock induce-able lambda red recombination system for MAGE (Multiplex Recombination Genome Engineering)DepositorBacterial ResistanceSpectinomycinAvailable sinceDec. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVB001
Plasmid#127185PurposeTES with RiboJ insulatorDepositorInsertTES with RiboJ Insulator
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVB003
Plasmid#127187PurposeTES without RiboJ insulatorDepositorInsertTES without RiboJ Insulator
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pTKRED
Plasmid#41062DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 7, 2013AvailabilityAcademic Institutions and Nonprofits only