Addgene

We narrowed to 202 results for: pter

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pEF-Bos MAL Flag

Plasmid

#41554

Depositor

Tom Maniatis, Kate Fitzgerald

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

MAL (TIRAP Human)

Use

Tags

6xHis and Flag

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 26, 2013

Availability

Academic Institutions and Nonprofits only
EYFP-Golgi-7

Plasmid

#56590

Purpose

Localization: Golgi, Excitation: 513, Emission: 527

Depositor

Michael Davidson

Article

Olenych et al Curr Protoc Cell Biol. 2007 Sep;Chapter

Insert

Golgi (B4GALT1 Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCMV-M2-TLR2-V660N

Plasmid

#12292

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

TLR2 (Tlr2 Mouse)

Use

Tags

FLAG (FLAG-M2)

Expression

Mammalian

Mutation

M82I, H202Y and V660N; signal peptide (aa1-23) re…

Promoter

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pCMV-M2-TLR2-P681H

Plasmid

#12290

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

TLR2 (Tlr2 Mouse)

Use

Tags

FLAG (FLAG-M2)

Expression

Mammalian

Mutation

A74V, M82I, H202Y and P681H; signal peptide (aa1-…

Promoter

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
IDA3::HA exon2 (WS13)

Plasmid

#126874

Purpose

p3xHA inserted within second exon of IDA3. Includes ampicilin and hygromycin resistance.

Depositor

Winfield Sale

Article

Hunter et al Mol Biol Cell. 2018 Apr 15;29(8):886-896

Insert

IDA3

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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IDA3::NeonGreen N-term (WS14)

Plasmid

#126875

Purpose

NeonGreen-tag attached to the N-terminus of IDA3. Includes ampicilin and hygromycin resistance.

Depositor

Winfield Sale

Article

Hunter et al Mol Biol Cell. 2018 Apr 15;29(8):886-896

Insert

IDA3

Use

Other

Tags

NeonGreen

Expression

Mutation

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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IDA3::NeonGreen exon2 (WS15)

Plasmid

#126876

Purpose

NeonGreen-tag embedded in the second exon of IDA3. Includes ampicilin and hygromycin resistance.

Depositor

Winfield Sale

Article

Hunter et al Mol Biol Cell. 2018 Apr 15;29(8):886-896

Insert

IDA3

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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mPA-GFP-Actin-7

Plasmid

#57120

Purpose

Localization: Actin, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Article

Olenych et al Curr Protoc Cell Biol. 2007 Sep;Chapter

Insert

Actin (ACTB Human)

Use

Tags

mPA-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pET22b-T5-sfGFP*-XcP4H-MH4R

Plasmid

#178043

Purpose

Express green fluorescent protein with TAG at 151 position and enzymes to biosynthesize 5HTP

Depositor

Han Xiao

Article

Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 1

Inserts

superfolder green fluorescent protein with TAG at 151 position
XcP4H with W179F mutation
dihydropterine reductase from human
pterin-4a-carbinolamine dehydratase from human

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-EPG

Plasmid

#117978

Purpose

Express EPG in mamalian cells

Depositor

Assaf Gilad

Article

Krishnan et al Sci Rep. 2018 Jun 8;8(1):8764. doi: 10.1

Insert

Electromagnetic perceptive gene

Use

Tags

HIS and v5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-2_dCrk

Plasmid

#131146

Purpose

Expresses GST::dCrk (based on crk isoform RA).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

Crk (Crk Fly)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX6P1 UBXN11

Plasmid

#169017

Purpose

Expresses GST-UBXN11 (human) in bacteria

Depositor

Hemmo Meyer

Article

Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

Insert

UBXN11 (UBXN11 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pMT2-MEKK3-K392A

Plasmid

#21585

Depositor

John Kyriakis

Article

Chadee et al Mol Cell Biol. 2002 Feb . 22(3):737-49.

Insert

mitogen-activated protein kinase kinase kinase 3 (MAP3K3 Human)

Use

Tags

Expression

Mammalian

Mutation

Lysine 392 to Arginine

Promoter

Available since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pCDNA3-myc- mCE(Del 25C)

Plasmid

#82472

Purpose

Expresses myc tag and mRNA capping enzyme without 25 amino acid from C-terminal

Depositor

Daniel Schoenberg

Article

Mukherjee et al PLoS Biol. 2014 Aug 19;12(8):e1001933. d

Insert

myc-mCE-C terminal deleted (Rngtt Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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Sandgrouse–insD

Plasmid

#191228

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al Mol Biol Evol. 2022 Dec 6:msac258. doi:

Inserts

ATP1A1
ATP1B1

Use

Tags

Expression

Insect

Mutation

removed aspartic acid at 121 in the protein seque…

Promoter

PH and p10

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pFL GST-UBXN2A

Plasmid

#169016

Purpose

Expresses GST-UBXN2A (human) in insect cells

Depositor

Hemmo Meyer

Article

Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

Insert

UBXN2A (UBXN2A Human)

Use

Tags

GST

Expression

Insect

Mutation

Promoter

polyhedrin promoter

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pET28P_dCrk

Plasmid

#131145

Purpose

Expresses 6XHis::dCrk (based on crk isoform RA).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

Crk (Crk Fly)

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3-biotin-myc- mCE

Plasmid

#82475

Purpose

Expresses mRNA capping enzyme containing N terminal biotin and myc tag

Depositor

Daniel Schoenberg

Article

Mukherjee et al PLoS Biol. 2014 Aug 19;12(8):e1001933. d

Insert

Biotin (Rngtt Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pEF-HA-TSAd-Y89XF

Plasmid

#84559

Purpose

mammalian expression of TSAd-Y89XF

Depositor

Anne Spurkland

Article

Granum et al J Biol Chem. 2008 Aug 8;283(32):21909-19

Insert

TSAd (SH2D2A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Y279F, Y290F, Y305F

Promoter

EF1a

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only
pET-Cb2

Plasmid

#53529

Purpose

Expresses an insect mitochondrial arginyl-tRNA synthetase in E.coli

Depositor

Gabor Igloi

Article

Leisinger et al Insect Biochem Mol Biol. 2013 Dec;43(12)

Insert

mitochondrial arginyl-tRNA synthetase

Use

Tags

His Tag and Thioredoxin

Expression

Bacterial

Mutation

changed Ser4 to Ala4 to permit NcoI cloning

Promoter

T7

Available since

July 31, 2014

Availability

Academic Institutions and Nonprofits only

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