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Plasmid#171056PurposehTERT dEx2-expressing splicing minigeneDepositorInserthTERT dEx2-nanoLuc
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
Plasmid#125228PurposeExpresses SPARK2 NanoLuc-βarrestin2-TEVp in mammalian cellsDepositorInsertNanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
UseAAVTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-gLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)
Plasmid#125225PurposeExpresses NanoLuc-β2AR-AsLOV2(V416L) in mammalian cellsDepositorInsertgLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)
UseAAVTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX208-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
Plasmid#125231PurposeFor generating SPARK2 NanoLuc-βarrestin2-TEVp lentivirusDepositorInsertNanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX208-gLuc sp-NanoLuc-15aa linker-ICAM-Nrxn3b-HA
Plasmid#125233PurposeExpresses "sender" trans-cellular NanoLuc in mammalian cellsDepositorInsertgLuc sp-NanoLuc-15aa linker-ICAM-Nrxn3b-HA
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-NanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp
Plasmid#125235PurposeExpresses SPARK2 NanoLuc-βarrestin2-TEVp (no HA) in mammalian cellsDepositorInsertNanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
NLS-GI-VP16-IRES-NLS-GAL4DBD-NanoLuc-FKF1
Plasmid#183399PurposeDual expression of GI-VP16 and GAL4DBD-NanoLuc-FKF1 in the nucleusDepositorInsertGI-VP16-IRES-GAL4DBD-NanoLuc-FKF1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-HA-β2AR-NNES-NanoLuc-15aa linker-AsLOV2(V416L)
Plasmid#125224PurposeExpresses β2AR-NanoLuc-AsLOV2(V416L) in mammalian cellsDepositorInsertHA-β2AR-NNES-NanoLuc-15aa linker-AsLOV2(V416L)
UseAAVTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-β2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5
Plasmid#125236PurposeExpresses modified SPARK β2AR-NanoLuc transmembrane component in mammalian cellsDepositorInsertβ2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5
UseAAVTagsFlag, V5ExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX208-gLuc sp-NanoLuc-HA-oβ2AR-TS-eLOV-TEVcs-Flag-Gal4
Plasmid#125230PurposeFor generating SPARK NanoLuc-oβ2AR transmembrane component lentivirusDepositorInsertgLuc sp-NanoLuc-HA-oβ2AR-TS-eLOV-TEVcs-Flag-Gal4
UseLentiviralTagsHA,FlagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-IgK sp-HA-CD4-10aa linker-CIBN-NNES-NanoLuc
Plasmid#125229PurposeExpresses transmembrane intracellular NanoLuc in mammalian cellsDepositorInsertIgK sp-HA-CD4-10aa linker-CIBN-NNES-NanoLuc
UseAAVTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)::NL
Plasmid#141289PurposeNLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)DepositorInsertNLUC
UseLuciferaseTagsExpressionBacterialMutationEngineered for high stability (t1/2 = 11.5 days a…Promoterpromoter for bacteriophage T7 RNA polymeraseAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)::NL3F10H
Plasmid#141290PurposeNLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)DepositorInsertNLUC3F10H
UseLuciferaseTagsFlag (x3) and His (x10)ExpressionBacterialMutationEngineered for high stability (t1/2 = 11.5 days a…Promoterpromoter for bacteriophage T7 RNA polymeraseAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)::MBP-NL3F10H
Plasmid#141291PurposeMBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)DepositorInsertMBP-NLUC3F10H
UseLuciferaseTagsFlag (x3), His (x10), and MBPExpressionBacterialMutationEngineered for high stability (t1/2 = 11.5 days a…Promoterpromoter for bacteriophage T7 RNA polymeraseAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a Gx-NL3
Plasmid#169108PurposeExpresses protein G fused to 3 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.DepositorInsertprotein G - NanoLuc - NanoLuc - NanoLuc
UseTagshis-tag and strep-tagExpressionBacterialMutationAla 24 stopPromoterT7Available sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-N-cG-Blast
Plasmid#124981PurposeExpress Nanoluc, copGFPDepositorInsertNanoluc, copGFP
UseLentiviralTagsExpressionMutationPromoterEF1-alphaAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-N-cG-Hph
Plasmid#124980PurposeExpress Nanoluc, copGFPDepositorInsertNanoluc, copGFP
UseLentiviralTagsExpressionMutationPromoterEF1-alphaAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-N-cG-Zeo
Plasmid#124979PurposeExpress Nanoluc, copGFPDepositorInsertNanoluc, copGFP
UseLentiviralTagsExpressionMutationPromoterEF1-alphaAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-TRE3G-N-tdT-CMV-P
Plasmid#192887PurposeTet-inducible Nanoluc-tdTomato expressionDepositorInsertNanoluc-P2A-tdTomato
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-N-cG-Neo
Plasmid#124978PurposeExpress Nanoluc, copGFPDepositorInsertNanoluc, copGFP
UseLentiviralTagsExpressionMutationPromoterEF1-alphaAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only