-
Plasmid#206282PurposeENTR Vector 2 for MultiSite Gateway assembly. Encodes mKO2-hCdt1 under the control of CMVDepositorInsertmKO2-hCdt1 (CDT1 Human)
UseMultimate/gateway entr 2TagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
KA2897_pPB-TREtight-UBCmin-cHA-IRESH2BmKO2
Plasmid#124176PurposepiggyBac-based Dox-inducible expression (moderate)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/Flag-mKO2-HuR.DelHNS
Plasmid#110373PurposeMammalian expression of mKO2-HuR (DelHNS) with FLAG tag, Flp-In T-Rex systemDepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseTagsFLAG and mKO2ExpressionMammalianMutationHNS (HuR nuclear-cytoplasmic shuttling sequence) …PromoterCMVAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLv-EF1a-Parkin(K161N)-A92mKO2
Plasmid#213554PurposeLentiviral vector for stable expression of K161N Parkin, internally tagged with mKO2DepositorInsertParkin (PRKN Human)
UseLentiviralTagsExpressionMammalianMutationK161N mutation in ParkinPromoterEF1aAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLv-EF1a-Parkin(R104A)-A92mKO2
Plasmid#213556PurposeLentiviral vector for stable expression of R104A Parkin, internally tagged with mKO2DepositorInsertParkin (PRKN Human)
UseLentiviralTagsExpressionMammalianMutationR104A mutation in ParkinPromoterEF1aAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLv-EF1a-Parkin(S65A)-A92mKO2
Plasmid#213557PurposeLentiviral vector for stable expression of S65A Parkin, internally tagged with mKO2DepositorInsertParkin (PRKN Human)
UseLentiviralTagsExpressionMammalianMutationS65A mutation in ParkinPromoterEF1aAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLv-CMV(trunc)-Parkin-A92mKO2
Plasmid#213549PurposeLentiviral vector for stable low expression of Parkin, internally tagged with mKO2DepositorInsertParkin (PRKN Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTruncated CMVAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLv-EF1a-Parkin(N273K)-A92mKO2
Plasmid#213555PurposeLentiviral vector for stable expression of N273K Parkin, internally tagged with mKO2DepositorInsertParkin (PRKN Human)
UseLentiviralTagsExpressionMammalianMutationN273K mutation in ParkinPromoterEF1aAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLv-EF1a-Parkin(W403A)-A92mKO2
Plasmid#213558PurposeLentiviral vector for stable expression of W403A Parkin, internally tagged with mKO2DepositorInsertParkin (PRKN Human)
UseLentiviralTagsExpressionMammalianMutationW403A mutation in ParkinPromoterEF1aAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQC V5 mKO2 HuR.wt IRES Puro
Plasmid#110387Purposeγ-Retroviral transfer vector for expressing HuR (wild type), V5 and mKO2 tags, IRES-driven Puromycin selection.DepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseRetroviralTagsV5 and mKO2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQC V5 mKO2 HuR.DelHNS IRES Puro
Plasmid#110389Purposeγ-Retroviral transfer vector for expressing HuR (delHNS), V5 and mKO2 tags, IRES-driven Puromycin selection.DepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseRetroviralTagsV5 and mKO2ExpressionMammalianMutationHNS (HuR nuclear-cytoplasmic shuttling sequence) …PromoterCMVAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
KA2896_pPB-TREtight-Tkmin-cHA-IRESH2BmKO2
Plasmid#124175PurposepiggyBac-based Dox-inducible expression (very weak)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA2895_pPB-TREtight-TATAmin-cHA-IRESH2BmKO2
Plasmid#124174PurposepiggyBac-based Dox-inducible expression (weak)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19 KGA HA5'-mKO2-P2A-Zeo-3'
Plasmid#110408PurposeHomology donor for glutaminase KGA isoform knock-in, mKO2-P2A-ZeoDepositorInsertGLS glutaminase (GLS Human)
UseCRISPRTagsExpressionMutationPromoternoneAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7m-Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)
Plasmid#83841PurposeFluorescent probe for G1/S transition and M/G1 transitionDepositorUseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTU-A-005
Plasmid#124412PurposeSlot A, contains mKO2-MarA (step 1)DepositorInsertslot A with mKO2-MarA reporter + terminator + spacer
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTU-A-010
Plasmid#124418PurposeSlot A, contains mKO2-MarA (step 1)DepositorInsertslot A with mKO2-MarA reporter + terminator + spacer
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBABE.puro/TO/Flag/HuR.S221D
Plasmid#110355PurposeDoxycycline inducible mammalian retroviral expression of HuR (S221D) with FLAG and mKO2 tagsDepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseRetroviralTagsFLAG and mKO2ExpressionMammalianMutationS221D, P237SPromotergagAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBD21_036
Plasmid#109153PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of mKO2DepositorInsertsmRFP
mKO2
UseSynthetic BiologyTagsExpressionBacterial and MammalianMutationPromoterCMV and pTetAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
BSP607
Plasmid#122252Purposeexpression vector for mKO2 under EFT3 promoterDepositorInsertmKO2
UseTagsExpressionWormMutationPromoterEFT3Available sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only