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pFRT-DestFLAGHA_RBPMS2
Plasmid#65748Purposeconstitutively expresses FLAG-HA-tagged RBPMS2 in mammalian cellsDepositorInsertRBPMS2 (RBPMS2 Human)
UseTagsFLAG HAExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pN-iRS3GG
Plasmid#98858PurposeExpresses pheS cassette (in place of an antisense RNA probe, for golden gate cloning) in E. coli (kanR).DepositorInsertPheS cassette to be selectively replaced by unique asRNA probes
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
myc-Med4 pBacPAK8
Plasmid#15364DepositorInsertMed4 (MED4 N/A, Human)
UseBaculovirus constructionTagsFLAGExpressionMutationPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestGFP_RBPMS
Plasmid#65744Purposeexpresses GFP-tagged RBPMS in mammalian cellsDepositorInsertRBPMS (RBPMS Human)
UseTagsGFPExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestRFP_RBPMS
Plasmid#65745Purposeexpresses RFP-tagged RBPMS in mammalian cellsDepositorInsertRBPMS (RBPMS Human)
UseTagsRFPExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS2
Plasmid#59390PurposeDestination vector with RBPMS2 for stable cell line generationDepositorInsertRBPMS2 (RBPMS2 Human)
UseTagsFLAG HA and V5ExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceFeb. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
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FLAG-Med9 pBacPAK8
Plasmid#15363DepositorInsertMed9 (Med9 Mouse)
UseBaculovirus constructionTagsFLAGExpressionMutationPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TripleColour-StAR
Plasmid#222695PurposeCompetition assayDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsTagBFP, miRFP703, dTomatoExpressionMammalianMutationPGK, TagBFP, dTomato without BsmBI cutsitePromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
P789_pY026_EnAsCpf1_CLYBL_T1_catRNA
Plasmid#202760PurposeEncodes for CRISPR-Cas12a (EnAsCpf1) with gRNA for targeting the CLYBL safe harbor siteDepositorInserthuAsCpf1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-aC-VTS-US
Plasmid#160425PurposeExpression of c-luciferase from sp. Vargula tsujii (USA)DepositorInsertluciferase
UseLuciferaseTags6x Histidine tag, Yeast alpha secretion factor, c…ExpressionYeastMutationSee depositor comments belowPromoterAOXAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-aC-MSVU-BZ
Plasmid#160426PurposeExpression of c-luciferase from sp. Maristella sp. SVU (Belize)DepositorInsertluciferase
UseLuciferaseTags6x Histidine tag, Yeast alpha secretion factor, c…ExpressionYeastMutationSee depositor comments belowPromoterAOXAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
hHSF1 S307A
Plasmid#118364PurposeThis plasmid expresses human HSF1 S307A mutant.DepositorInserthHSF1 (HSF1 Human)
UseTagsmyc and 6xHISExpressionMammalianMutationS307APromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestGFP_RBPMS2
Plasmid#65747Purposeexpresses GFP-tagged RBPMS2 in mammalian cellsDepositorInsertRBPMS2 (RBPMS2 Human)
UseTagsGFPExpressionMammalianMutationS13N mutation in RBPMS2PromoterCMV, 2x TetAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNK3203
Plasmid#219749PurposeDVK_AF CIDAR vector encoding hispidin-synthase from Mycena citricolor under control of CMV promoter, for mammalian expressionDepositorInsertpCMV - mcitHispS - tSV40
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT2-TetR-neoR
Plasmid#153310PurposeTet-repressor with neomycin selection marker, to be used together with pT2-CMV/TetO2-EYFP-GWDepositorInsertTetR
UseTransposonTagsExpressionMutationPromoterSV40Available sinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNK5712
Plasmid#219752PurposepGAP-like vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 under control of GAP promoter, for yeast expressionDepositorInsertpGAP - nnH3H_v2 - tAOX
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK5785
Plasmid#219753PurposepGAP-like vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 under control of GAP promoter, for yeast expressionDepositorInsertpGAP - nnLuz_v4 - tAOX
UseLuciferaseTagsExpressionYeastMutationPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
hHSF1 S303/7A
Plasmid#118365PurposeThis plasmid expresses human HSF1 S303A and S307A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.DepositorInserthHSF1 (HSF1 Human)
UseTagsmyc and 6xHISExpressionMammalianMutationS303A and S307APromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only
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