Addgene

We narrowed to 699 results for: ha/1000

Showing: 101 - 120 of 699 results

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GFP Cav2.2 HA pCAGGS

Plasmid

#206054

Purpose

expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Macabuag et al J Neurosci. 2015 Oct 28;35(43):14636-52.

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV/B-actin

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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GFP Cav2.2 HA pcDNA3

Plasmid

#206053

Purpose

expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Macabuag et al J Neurosci. 2015 Oct 28;35(43):14636-52.

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-HsAGO2_E

Plasmid

#146224

Purpose

Insect Expression of HsAGO2

Depositor

Elisa Izaurralde

Article

Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

Insert

HsAGO2 (AGO2 Human)

Use

Tags

Expression

Insect

Mutation

Contains a K660E mutation compared to the sequenc…

Promoter

Available since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-p53 2KR

Plasmid

#78799

Purpose

To overexpress p53 2KR in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

Insert

p53 (TP53 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K291R, K292R

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only
pIRESneo-FLAG/HA Ago2

Plasmid

#10821

Depositor

Thomas Tuschl

Article

Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

Insert

Argonaute 2 (AGO2 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

R668H. Additionally, N terminus different from wi…

Promoter

Available since

Oct. 14, 2005

Availability

Academic Institutions and Nonprofits only

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HIS-HA-CMYC P53

Plasmid

#233738

Purpose

expresses HIS-HA-CMYC triple tagged P53 for pulldown assay

Depositor

Albert Keung

Article

Han et al Methods. 2025 Mar;235:92-99. doi: 10.101

Insert

HIS-HA-CMYC-p53 (TP53 Human)

Use

Tags

HIS-HA-CMYC

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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Cav2.2 HA W391A pcDNA3

Plasmid

#206071

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2

Depositor

Annette Dolphin

Article

Waithe et al J Biol Chem. 2011 Mar 18;286(11):9598-61

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

W391A

Promoter

CMV

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-LMW-FGF2

Plasmid

#157663

Purpose

Expresses FGF2 in mammalian cell

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Fibroblast growth factor 2 (FGF2 Human)

Use

Tags

HA tag

Expression

Mammalian

Mutation

deleted amino acid 1-134

Promoter

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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HA-Rab11-DN (S25N)

Plasmid

#101046

Purpose

Expresses HA-tagged human Rab11-DN (S25N) in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

RAB11 (RAB11A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

S25N

Promoter

CMV

Available since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe JUND-HA neo

Plasmid

#58489

Purpose

mammalian expression of human JUND

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

JUND (JUN Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2016

Availability

Academic Institutions and Nonprofits only

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Cav2.2 D122A HA pCAGGS

Plasmid

#206097

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

Depositor

Annette Dolphin

Article

Dahimene et al Cell Rep. 2018 Nov 6;25(6):1610-1621.e5.

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

D122A

Promoter

CMV/B-actin

Available since

Nov. 22, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 HA D122A pcDNA3

Plasmid

#206108

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

Depositor

Annette Dolphin

Article

Dahimene et al Cell Rep. 2018 Nov 6;25(6):1610-1621.e5.

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

D122A

Promoter

CMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pWZLblasti-HA-MEK1 C121S

Plasmid

#53165

Purpose

Expresses HA tagged human MEK1 C121S from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (MAP2K1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

C121S

Promoter

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
pGEX6P1-HA-MEK1 C121S

Plasmid

#53164

Purpose

Expresses GST-HA tagged human MEK1 C121S from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (MAP2K1 Human)

Use

Tags

GST and HA

Expression

Bacterial

Mutation

C121S

Promoter

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.2-Cx43-ML-HA

Plasmid

#49857

Purpose

Encodes human connexin 43 with all internal methionines mutated to leucine and a C_terminal HA epitope fusion tag

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

Connexin 43 (GJA1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

All internal Methionines mutated to Leucine. Cont…

Promoter

CMV

Available since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only
pGEX6P1-HA-MEK1 CBM

Plasmid

#53163

Purpose

Expresses GST-HA tagged human MEK1 M187AH188AM230AH239A from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (MAP2K1 Human)

Use

Tags

GST and HA

Expression

Bacterial

Mutation

M187A/H188A/M230A/H239A

Promoter

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
cacna2d1 HA D491A pcDNA3

Plasmid

#206070

Purpose

expression of rat calcium channel alpha2delta-1 with a single HA tag inserted after aa549 and a D491A mutation that prevents Gabapentin binding

Depositor

Annette Dolphin

Article

Gumerov et al Proc Natl Acad Sci U S A. 2022 Mar 8;119

Insert

cacna2d1 (Cacna2d1 Rat)

Use

Tags

Expression

Mammalian

Mutation

D491A

Promoter

CMV

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-UBXD8-Strep-HA

Plasmid

#113477

Purpose

Expression of human UBXD8 with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

UBXD8 (FAF2 Human)

Use

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pWZLblasti-HA-MEK1-MEK5DD

Plasmid

#53177

Purpose

Expresses HA tagged chimeric human MEK1 (nucleotides 1–201)-MEK5 (498–1344 with S311DT315D) from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (1-201)-MEK5 (498–1344) fusion (MAP2K1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

MEK1 (1-201) MEK5(498–1344 with S311D/T315D)

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only
cacna2d1 R241A HA pMT2

Plasmid

#206111

Purpose

expression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549 and with an R421A mutation that disrupts Gabapentin binding

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna2d1 (Cacna2d1 Rat)

Use

Tags

Expression

Mammalian

Mutation

R241A

Promoter

Ad MLP/TPL/SV40

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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