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Plasmid#108758PurposeExpresses aa169 to C-terminus of Flp recombinase fused to FKBP CIDDepositorInsertFlp recombinase fragment aa169 to C-terminus
UseCre/Lox and Synthetic BiologyTagsFKBP and NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2280_pCAG-PV1-FlpO-N374-L1-FRB-NLS-BGHpA
Plasmid#108759PurposeExpresses N-terminus to aa374 of Flp recombinase fused to FRB CIDDepositorInsertFlp recombinase fragment N-terminus to aa374
UseCre/Lox and Synthetic BiologyTagsFRB,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2281_pCAG-PV1-FKBP-L1-FlpO-375C-NLS-BGHpA
Plasmid#108760PurposeExpresses aa375 to C-terminus of Flp recombinase fused to FKBP CIDDepositorInsertFlp recombinase fragment aa375 to C-terminus
UseCre/Lox and Synthetic BiologyTagsFKBP and NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2284_pCAG-PV1-FlpO-N396-L1-FRB-NLS-BGHpA
Plasmid#108761PurposeExpresses N-terminus to aa396 of Flp recombinase fused to FRB CIDDepositorInsertFlp recombinase fragment N-terminus to aa396
UseCre/Lox and Synthetic BiologyTagsFRB,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2285_pCAG-PV1-FKBP-L1-FlpO-397C-NLS-BGHpA
Plasmid#108762PurposeExpresses aa397 to C-terminus of Flp recombinase fused to FKBP CIDDepositorInsertFlp recombinase fragment aa397 to C-terminus
UseCre/Lox and Synthetic BiologyTagsFKBP and NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2164_pCAG-PV1-FlpO-N168-L1-GID1-NLS-BGHpA
Plasmid#108749PurposeExpresses N-terminus to aa168 of Flp recombinase fused to GID1 CIDDepositorInsertFlp recombinase fragment N-terminus to aa168
UseCre/Lox and Synthetic BiologyTagsGID1,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2184_pCAG-PV1-GAI-L1-FlpO-169C-NLS-BGHpA
Plasmid#108750PurposeExpresses aa169 to C-terminus of Flp recombinase fused to GAI CIDDepositorInsertFlp recombinase fragment aa169 to C-terminus
UseCre/Lox and Synthetic BiologyTagsGAI and NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2173_pCAG-PV1-FlpO-N374-L1-GID1-NLS-BGHpA
Plasmid#108751PurposeExpresses N-terminus to aa374 of Flp recombinase fused to GID1 CIDDepositorInsertFlp recombinase fragment N-terminus to aa374
UseCre/Lox and Synthetic BiologyTagsGID1,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2193_pCAG-PV1-GAI-L1-FlpO-375C-NLS-BGHpA
Plasmid#108752PurposeExpresses aa375 to C-terminus of Flp recombinase fused to GAI CIDDepositorInsertFlp recombinase fragment aa375 to C-terminus
UseCre/Lox and Synthetic BiologyTagsGAI and NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2614_pCAG-PV1-GAI-L2-28-396-FlpO-ABI-NLS-BGHpA
Plasmid#108833PurposeExpresses aa28 to aa396 of Flp recombinase fused to GAI and ABI CIDsDepositorInsertFlp recombinase fragment aa28 to aa396
UseCre/Lox and Synthetic BiologyTagsABI,NLS and GAIExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV:ITR-U6-sgRNA (Backbone) PCB-FlPO-WPRE-syntetisk pA-UTR
Plasmid#68347PurposeAAV plasmid expressing the FlpO recombinase. with empty gRNADepositorInsertFlPO
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2 probasin_mTQ2_FlpO
Plasmid#68405PurposeThe prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex8 and SMAD4 ex2. are expressed by the U6 promoterDepositorInserts3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2
FlpO
UseCRISPRTagsmTurquoise2 (BFP)ExpressionMammalianMutationPromoterSynthetic Probasin ARRx2 and U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
AiP14826: pAAV-AiE0441h_3xC2-minBG-FlpO-WPRE3-BGHpA (Alias: CN4826)
Plasmid#214863PurposeExpression of FlpO in striatal medium spiny neuronsInsertFlpO
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiP14201: pAAV-AiE0779m_3xC2-minBG-FlpO-WPRE3-BGHpA (Alias: CN4201)
Plasmid#214855PurposeExpression of FlpO in striatal direct pathway medium spiny neurons (D1 MSNs)InsertFlpO
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC18 Apln5-HA-FlpO-pA-LoxP-PGK-Neo-pA-LoxP-Apln3 (SO89)
Plasmid#159222PurposeCan be used to target the mouse Apln locus in mouse eggs or ES cells, in order to drive the mosaic expression of the protein HA-NLS-FlpODepositorInsertApln 5'-FlpO-WPRE-Sv40pA-LoxP-PGK-Neo-pA-LoxP-Apln
UseTagsExpressionMammalianMutationPromoterAplnAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
AiP12205 - pAAV-3xSP10ins-AiE0367h-minRho-FlpO-WPRE3-BGHpA (Alias: CN2205)
Plasmid#224160PurposeAiE0367h is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal MSNsInsertFlpO
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7 probasin_mTQ2_FlpO
Plasmid#68356Purpose-The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and SMAD4 ex2. are expressed by the U6 promoterDepositorInserts4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7
FlpO recombinase
UseCRISPRTagsmTurquoise2 (BFP)ExpressionMammalianMutationPromoterSynthetic Probasin ARRx2 and U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9 probasin_mTQ2_FlpO
Plasmid#68357PurposeThe prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and 8, and SMAD4 ex2 and ex 9 are expressed by the U6 promoter.DepositorInserts5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9
FlpO recombinase
UseCRISPRTagsmTurquoise2 (BFP)ExpressionMammalianMutationPromoterU6 and synthetic Probasin ARRx2Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flpo (AAV1)
Viral Prep#55637-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-EF1a-Flpo (#55637). In addition to the viral particles, you will also receive purified pAAV-EF1a-Flpo plasmid DNA. EF1a-driven Flpo recombinase expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsNoneAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FlpO (AAV1)
Viral Prep#183412-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-CAG-FlpO (#183412). In addition to the viral particles, you will also receive purified pAAV-CAG-FlpO plasmid DNA. CAG-driven expression of the recombinase FlpO. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsNoneAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only