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Plasmid#174140PurposeCMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarletDepositorInserthuman codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-mScarlet
UseIn vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-mScarletExpressionMammalianMutationSpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…PromoterCMV and T7Available sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
C413-E34: attB4-mCMVp>-attB1r
Plasmid#162926PurposeGateway attB4/attB1r entry clone for mammalian expression using the minimal CMV promoterDepositorInsertminimal CMV IE1 promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
C453-E04: attB4-CMV51p>-attB5
Plasmid#162973PurposeGateway attB4/attB5 entry clone for mammalian expression using an optimized human CMV promoterDepositorInsertoptimized human CMV promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
C413-E36: attB4-CMV51p>-attB1r
Plasmid#162927PurposeGateway attB4/attB1r entry clone for mammalian expression using an optimized human CMV promoterDepositorInsertoptimized CMV promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SpdCas9-VP64
Plasmid#115794PurposeExpresses SpdCas9-Vp64 fusion protein when packaged into AAVDepositorInsertVP64
UseAAVTags3XFLAG and dCas9-VP64ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCMV-flag-Caspase11
Plasmid#21145DepositorInsertCaspase11 (Casp4 Mouse)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9-VRER-P2A-mScarlet (MNW003)
Plasmid#174138PurposeCMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarletDepositorInserthuman codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-mScarlet
UseIn vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-mScarletExpressionMammalianMutationVRER=D1135V/G1218R/R1335E/T1337RPromoterCMV and T7Available sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9-VRQR-P2A-mScarlet (MNW004)
Plasmid#174139PurposeCMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarletDepositorInserthuman codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-mScarlet
UseIn vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-mScarletExpressionMammalianMutationVRQR=D1135V/G1218R/R1335Q/T1337RPromoterCMV and T7Available sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-T7-hOsa1
Plasmid#17986DepositorInsertOsa1 (ARID1A Human)
UseTagsT7 epitopeExpressionMammalianMutationMissing 5' endPromoterAvailable sinceAug. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAdx-CMV-copGFP
Plasmid#73346PurposeExpress copGFP under CMV promoterDepositorInsertCMV-copGFP
UseAdenoviralTagsExpressionMutationPromoterCMVAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAdx-CMV-tdTomato
Plasmid#73347PurposeExpress tdTomato under CMV promoterDepositorInsertCMV-tdTomato
UseAdenoviralTagsExpressionMutationPromoterCMVAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCMV3
Plasmid#85711PurposeExpression vector employing the mouse CMV major immediate-early promoter enhancer (MIEP); there is a small polylinker (PstI, SmaI, XbaI, SalI ,HpaI) followed by the SV40 polyA signal sequencesDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterMIEPmouse CMV MIEPAvailable sinceApril 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPD1029 ZF43x6-Compact(CMVMin)_EYFP
Plasmid#138913PurposeEYFP reporter vector for the ZF43x6-Compact(overlapping ZF37) promoter with a CMV minimal promoterDepositorInsertZF43x6-Compact(CMVmin)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF43x6-Compact(CMVmin)Available sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-sadCas9-VP64
Plasmid#115790PurposeAn AAV vector expressing S. aureus dCas9 fused to VP64.DepositorInsertSa-dCas9
UseAAVTagsNLS and NLS-VP64ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAdx-CMV-LacZ
Plasmid#73345PurposeExpress LacZ under control of CMV promoterDepositorInsertCMV-LacZ
UseAdenoviralTagsExpressionMutationPromoterCMVAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-hBMPR-1A CA
Plasmid#49527Purposeexpresses constitutively active human BMP receptor 1ADepositorInsertBMPR-1A CA (BMPR1A Human)
UseTagsExpressionMammalianMutationconstitutively active mutation (Q233D); also P2A …PromoterCMVAvailable sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMVTNT-EPHX1
Plasmid#53114PurposeIn vitro translation of microsomal epoxide hydrolaseDepositorInsertEPHX1 (EPHX1 Human)
UseTagsExpressionBacterial and MammalianMutationPromoterCMV and T7Available sinceMay 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBSU6-shTim23/CMV-eGFP
Plasmid#89795PurposeExpresses shRNA against TIM23 with a separate promoter for expression of GFP.DepositorInsertshRNA Tim23 (Timm23 Mouse)
UseTagseGFP under separate promoterExpressionMammalianMutationPromoterU6Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
EF.delmFasL.CMV.GFP
Plasmid#17620DepositorInsertdelFasL (Fasl Mouse)
UseLentiviralTagsExpressionMammalianMutationNoncleavable mouse delFasL . Deleted the exon 2 r…PromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.HI.eGFP-Cre.WPRE.SV40
Plasmid#105545PurposeAAV expression of EGFP-Cre from CMV promoterDepositorHas ServiceAAV1, AAV2, AAV5, AAV8, and AAV9InsertEGFP-Cre
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterHIAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only