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Plasmid#131736PurposeExpresses Opti-SpCas9 in mammalian cellsDepositorInsertOpti-SpCas9
UseLentiviralTagsFlagExpressionMutationR661A+K1003HPromoterEFSAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
D3 clone (p21/WAF1 promoter)
Plasmid#16452DepositorInsertWAF1 promoter (CDKN1A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR v2-sgAMPK alpha1 clone3
Plasmid#162121PurposeLentiviral sgRNA plasmid targeting human AMPK alpha1DepositorInsertsgAMPK alpha1 (PRKAA1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
XE40 pSP64T-PABP (Clone 63)
Plasmid#17019DepositorInsertPoly(A) binding protein (pabpc1.S Frog)
UseXenopus expressionTagsExpressionMutationPromoterAvailable sinceApril 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJT309 RapInducible1-ActivatorCloneSite-Citrine
Plasmid#187321PurposeRapamycin inducible genetic circuit with cloning site for activator domainsDepositorArticleTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT2/shαSMA/GFP4_Seq1/clone1
Plasmid#206138PurposeKnockdown of alpha-SMA. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertalpha-SMA (Acta2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/shαSMA/GFP4_Seq2/clone1
Plasmid#201402PurposeKnockdown of alpha-SMA. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertalpha-SMA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
HH13 Clone 13 heavy chain
Plasmid#192177PurposeClone 13 heavy chain (HH013)DepositorInsertClone 13 heavy chain (HH013)
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR v2-sgAMPK alpha2 clone2
Plasmid#162123PurposeLentiviral sgRNA plasmid targeting human AMPK alpha2DepositorInsertsgAMPK alpha2 (PRKAA2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR v2-sgAMPK alpha2 clone3
Plasmid#162124PurposeLentiviral sgRNA plasmid targeting human AMPK alpha2DepositorInsertsgAMPK alpha2 (PRKAA2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAWp63-clone3-12 (OptiHF-SpCas9)
Plasmid#131737PurposeExpresses OptiHF-SpCas9 in mammalian cellsDepositorInsertOptiHF-SpCas9
UseLentiviralTagsFlagExpressionMutationQ695A+K848A+E923M+T924V+Q926APromoterEFSAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS Matrilin-4 (clone 1_35)
Plasmid#19192DepositorInsertMatrilin 4 isoform 1 precursor (MATN4 Chicken)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBS Matrilin-4 (clone 2_49)
Plasmid#19218DepositorInsertMatrilin 4 isoform 1 precursor (MATN4 Chicken)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
M35 Dpr yeast clone
Plasmid#17191DepositorInsertXDpr (dact1-b Frog)
UseTagsGal4-ADExpressionYeastMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
RAS Pathway 2.0 Clone Collection
Plasmid Kit#1000000070PurposeCollection contains 180 genes in the âRAS pathway version 2.0â, a crowdsourced pathway map of the RAS signaling pathway; For use with Gateway cloning or FNLCR Combinatorial Cloning PlatformDepositorAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Bison sgRNA library
Pooled Library#169942PurposeThe BISON CRISPR library targets 713 E1, E2, and E3 ubiquitin ligases, deubiquitinases, and control genes and contains 2,852 guide RNAs. It was cloned into the pXPR003.DepositorUseLentiviralAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXR003: CasRx gRNA cloning backbone
Plasmid#109053PurposehU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
RNA motif plasmid cloning backbone
Plasmid#107253PurposeRNA motif plasmid with BoxB sites. Digest with BsmBI and insert RNA motif of interest. Stuffer size 1.8kbDepositorInsertRNA motif plasmid cloning backbone
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 GFP LIC cloning vector (6D)
Plasmid#30127DepositorTypeEmpty backboneUseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 mCherry LIC cloning vector (6B)
Plasmid#30125PurposeEmpty LIC vector; adds an mCherry gene to the C-terminus of your protein of interest.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only