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Showing: 101 - 120 of 13452 results

  2. p425_Cas9_gRNA_LEU_1014a

    Plasmid
    #87407
    Purpose
    p425_Cas9_gRNA-ARS1014a All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, LEU2 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1014a sequence TTATGTGCGTATTGCTTTCA in yeast chromosome 10.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1014a
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87407-plasmid-map-sequence-id-193449

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  10. pENTR_Wheat_live_Cas9

    Plasmid
    #126880
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and BpiI sites for sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126880-plasmid-map-sequence-id-249730

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  13. pTPGI_dCas9_VP64

    Plasmid
    #49013
    Purpose
    encodes yeast-optimized dCas9_VP64 synthetic transcription factor
    Depositor
    Timothy Lu
    Article
    Farzadfard et al ACS Synth Biol. 2013 Sep 11.
    Insert
    dCas9_VP64 (codon-optimized for expression in S. cerevisiae)
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    Promoter
    pTPGI (galactose+aTc inducible)
    Available since
    Jan. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    49013-plasmid-map-sequence-id-231494

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  14. pKCcas9dO

    Plasmid
    #62552
    Purpose
    High efficiency Streptomyces genome editing by CRISPR/Cas9 system
    Depositor
    Weihong Jiang
    Article
    Huang et al Acta Biochim Biophys Sin (Shanghai). 201
    Inserts
    Scocas9
    sgRNA ()
    act-orf4 homology arm
    Use
    CRISPR; Bacterial genome editing
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    j23119 and ptipA
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    62552-plasmid-map-sequence-id-237466

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  16. pCMV_dCas9_VP64

    Plasmid
    #49015
    Purpose
    encodes human-optimized dCas9_VP64 synthetic transcription factor
    Depositor
    Timothy Lu
    Article
    Farzadfard et al ACS Synth Biol. 2013 Sep 11.
    Insert
    dCas9_VP64 (human-codon-optimized)
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    49015-plasmid-map-sequence-id-79086

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  19. pAG1guide_RUBY_EggCas9

    Plasmid
    #225983
    Purpose
    To make mutant alleles of AtAGAMOUS1 gene in Arabidopsis thaliana. The transgenics can be selected by red color appearance of plants
    Depositor
    Imran Siddiqi
    Insert
    Guide RNA against AtAGAMOUS1
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    35s promoter to Drive RUBY and DD45p to drive Cas9
    Available since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    225983-plasmid-map-sequence-id-448020

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Showing: 101 - 120 of 13452 results