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Showing: 101 - 120 of 13288 results

  2. pSaCas9_GFP

    Plasmid
    #64709
    Purpose
    Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells
    Depositor
    Kiran Musunuru
    Insert
    SaCas9-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP and 3xFLAG-SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    64709-plasmid-map-sequence-id-322473

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  3. pCAS9c

    Plasmid
    #141250
    Purpose
    Constitutive expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Article
    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1 (Cas9), pSNR52 (gRNA)
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141250-plasmid-map-sequence-id-308875

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  6. pDual_dsCas9_Blast_hs_NC_chr1

    Plasmid
    #214690
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214690-plasmid-map-sequence-id-428775

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  7. pDual_dsCas9_Blast_hs_TP73

    Plasmid
    #214691
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human TP73
    Depositor
    Kevin Janes
    Insert
    dgRNA_TP73 (TP73 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214691-plasmid-map-sequence-id-428772

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  10. pCas9

    Plasmid
    #167547
    Purpose
    Used for CRISPR-Cas9 mediated recomineering in Enterococcus. Can clone desired gRNA using BsaI digestion.
    Depositor
    Howard Hang
    Article
    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00
    Inserts
    chloramphenicol acetyl transferase
    pUC19 origin of replication
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    167547-plasmid-map-sequence-id-332733

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  12. pCas9D10A_GFP

    Plasmid
    #44720
    Purpose
    Co-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cells
    Depositor
    Kiran Musunuru
    Article
    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.
    Insert
    Cas9D10A-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP
    Expression
    Mammalian
    Mutation
    D10A mutation- nickase mutant
    Promoter
    CAG
    Available since
    May 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    44720-plasmid-map-sequence-id-179979

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  13. pCAS9i

    Plasmid
    #141249
    Purpose
    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Article
    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141249-plasmid-map-sequence-id-308874

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Showing: 101 - 120 of 13288 results