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Plasmid#146960PurposeInsect Expression of DmAGO1-R937ADepositorInsertDmAGO1-R937A (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations V315A and H…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-R937D_M
Plasmid#146961PurposeInsect Expression of DmAGO1-R937DDepositorInsertDmAGO1-R937D (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations V315A and H…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-D661E-siRNAres_J
Plasmid#146713PurposeInsect Expression of DmAGO1-D661E-siRNAresDepositorInsertDmAGO1-D661E-siRNAres (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations compared to…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-D661K-siRNAres_J
Plasmid#146714PurposeInsect Expression of DmAGO1-D661K-siRNAresDepositorInsertDmAGO1-D661K-siRNAres (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations compared to…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-K657E-siRNAres_J
Plasmid#146715PurposeInsect Expression of DmAGO1-K657E-siRNAresDepositorInsertDmAGO1-K657E-siRNAres (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations compared to…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-Y653L-siRNAres_J
Plasmid#146716PurposeInsect Expression of DmAGO1-Y653L-siRNAresDepositorInsertDmAGO1-Y653L-siRNAres (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations compared to…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-K690A_M
Plasmid#146957PurposeInsect Expression of DmAGO1-K690ADepositorInsertDmAGO1-K690A (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations V315A and H…PromoterAvailable sinceFeb. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
HCA1-Tango
Plasmid#66395PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertHCA1 (HCAR1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
RCAS-sgATG7
Plasmid#75228PurposeAn adaption on the RCAS/tv-a somatic cell gene transfer system, for use in combination with an existing Cas9 background in the cell/mouse of interest. Depositors: Jane Fraser/Noor GammohDepositorInsertAutophagy Related 7 (Atg7 Mouse)
UseRetroviralTagsExpressionMutationPromoterU6Available sinceJune 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-del942-971-GGSG_M
Plasmid#146950PurposeInsect Expression of DmAGO1-del942-971-GGSGDepositorInsertDmAGO1-del942-971-GGSG (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations V315A and H…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-del942-971-GSG_M
Plasmid#146951PurposeInsect Expression of DmAGO1-del942-971-GSGDepositorInsertDmAGO1-del942-971-GSG (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations V315A and H…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-del835-886-GGSG_M
Plasmid#146948PurposeInsect Expression of DmAGO1-del835-886-GGSGDepositorInsertDmAGO1-del835-886-GGSG (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations V315A and H…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-del835-886-GSG_M
Plasmid#146949PurposeInsect Expression of DmAGO1-del835-886-GSGDepositorInsertDmAGO1-del835-886-GSG (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations V315A and H…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-L819Y-siRNAres_AC
Plasmid#148394PurposeInsect Expression of DmAGO1-L819Y-siRNAresDepositorInsertDmAGO1-L819Y-siRNAres (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations compared to…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-P714GA744FL819Y-siRNAres_AC
Plasmid#148395PurposeInsect Expression of DmAGO1-P714GA744FL819Y-siRNAresDepositorInsertDmAGO1-P714GA744FL819Y-siRNAres (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 silent mutations compared to the…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVNP2.0-SpCas9
Plasmid#206880PurposeExpresses FLAG-tagged SpCas9 fused to the N-terminus of Gag/GagPol harbouring an intervening phospholipase C-δ1 pleckstrin homology (PH) domainDepositorInsertSpCas9 (cas9 )
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterCMVAvailable sinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast mAtg7
Plasmid#173170PurposeRetroviral vector to express sgRNA resistant mouse Atg7DepositorInsertAutophagy Related 7 (Atg7 Mouse)
UseRetroviralTagsExpressionMutationSynonymous mutations at sgRNA sitesPromoterMMLVAvailable sinceFeb. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
EMMA toolkit
Plasmid Kit#1000000119PurposeUsed for the construction of a variety of mammalian expression vectors in a one-pot, one-step Golden Gate reaction; compatible with automated productionDepositorAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
FNL Combinatorial Cloning Platform
Plasmid Kit#1000000211PurposeA collection of Multisite Gateway Entry clone plasmids which can be used in a combinatorial fashion to generate large numbers of tagged expression clones for protein expression in a variety of systemsDepositorAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only