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We narrowed to 253 results for: Weissman Jonathan

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pGFP-AGG-IRES-mCherry

Plasmid

#222115

Purpose

Start codon reporter with mutated start codon

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

GFP-IRES-mCherry

Use

Lentiviral

Tags

Expression

Mutation

AGG start codon

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGFP-AUG-IRES-mCherry

Plasmid

#222109

Purpose

Start codon reporter (WT AUG)

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

GFP-IRES-mCherry

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGFP-AUC-IRES-mCherry

Plasmid

#222117

Purpose

Start codon reporter with mutated start codon

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

GFP-IRES-mCherry

Use

Lentiviral

Tags

Expression

Mutation

AUC start codon

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1_dCas9-XTEN-KRAB-p2A-GFP

Plasmid

#222108

Purpose

Donor vector for CRISPRi integration at AAVS1 with a GFP fluorophore

Depositor

Jonathan Weissman

Article

She et al Cell. 2023 Jul 6;186(14):2977-2994.e23.

Insert

CRISPRi-kox1(KRAB) (cas9 S. pyogenes, Human)

Use

AAV and CRISPR

Tags

HA and P2A-GFP

Expression

Mammalian

Mutation

D10A, H840A

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pENN491_TALEcharm_MmT8x

Plasmid

#220836

Purpose

TALEcharm Kv2 expression in mammalian cells; targets Prnp

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

TALEcharm

Use

Lentiviral and TALEN

Tags

P2A-TagBFP

Expression

Mammalian

Mutation

N/A

Promoter

EF1a

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pENN456_TALEcharm_Kv2_MmT8x

Plasmid

#220835

Purpose

TALEcharm expression in mammalian cells

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

TALEcharm Kv2

Use

Lentiviral and TALEN

Tags

P2A-TagBFP

Expression

Mammalian

Mutation

N/A

Promoter

EF1a

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pTMB398_ZFcharm_Prnp_SMM

Plasmid

#220845

Purpose

AAV genome expressing ZFcharm targeting Prnp; single mismatch self-silencing binding site

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm

Use

AAV

Tags

HAtag

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pTMB303_ZFcharm_Kv1_Prnp_SMM

Plasmid

#220841

Purpose

AAV genome expressing ZFcharm Kv1 targeting Prnp; single mismatch self-silencing binding site

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm Kv1

Use

AAV

Tags

HAtag

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pGM9

Plasmid

#220167

Purpose

Expresses a reporter for an alpha-helical OMM protein

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

FUNDC1 (FUNDC1 Human)

Use

Lentiviral

Tags

GFP11

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pGM238

Plasmid

#220178

Purpose

Expresses exogenous ribosomal-binding domain mutant NAC complex members (K78E NACA and K43E BTF3)

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

NACA and BTF3 (NACA, BTF3 Human)

Use

Lentiviral

Tags

3xFLAG (NACA); 6xHis (BTF3)

Expression

Mutation

K78E (NACA) + K43B (BTF3)

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pGM258

Plasmid

#220176

Purpose

Expresses exogenous C-terminal mutant TTC1 (I271D)

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TTC1 (TTC1 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

I271D

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pGM133

Plasmid

#220173

Purpose

CRISRPi guide RNA against TTC1

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TTC1 (sgRNA) (TTC1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGM84

Plasmid

#220172

Purpose

CRISRPi guide RNA against BTF3

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

BTF3 (sgRNA) (BTF3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGM268

Plasmid

#220181

Purpose

Expresses exogenous C-terminal mutant TTC1 (I271D) with an N-terminal sfGFP tag

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TTC1 (TTC1 Human)

Use

Lentiviral

Tags

sfGFP

Expression

Mutation

I271D

Promoter

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGM295

Plasmid

#220183

Purpose

Expresses exogenous wild-type SEC61B with a C-terminal sfGFP tag

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

SEC61B (SEC61B Human)

Use

Lentiviral

Tags

sfGFP

Expression

Mutation

Promoter

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGM265

Plasmid

#220179

Purpose

Expresses exogenous wild-type TTC1 with an N-terminal sfGFP tag

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TTC1 (TTC1 Human)

Use

Lentiviral

Tags

sfGFP

Expression

Mutation

Promoter

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGM216

Plasmid

#220175

Purpose

Expresses exogenous TPR mutant TTC1 (R190A_R194A)

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TTC1 (TTC1 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

R190A + R194A

Promoter

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGM209

Plasmid

#220174

Purpose

Expresses exogenous wild-type TTC1

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TTC1 (TTC1 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW1509

Plasmid

#62363

Purpose

pFA6a BirA-heh2 linker-SSH1::HIS5

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

BirA-heh2-Ssh1

Use

Tags

Expression

Yeast

Mutation

Promoter

Gal1

Available since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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pJW1668

Plasmid

#112042

Purpose

CEN/ARS plasmid for GAL-inducible FET3-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

FET3

Use

Tags

V5-TEV-EGFP

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge