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S4246

Bacterial Strain

#156372

Purpose

Erythromycin-resistant S2060 derivative

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Bacterial Resistance

Streptomycin, tetracycline, erythromycin

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only
pPB UbC Arrestin-uTEV2-V5; UAS-mCherry

Plasmid

#173123

Purpose

Expresses uTEV2 and reporter receiver construct in DIPG

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

Arrestin-uTEV2-V5; UAS-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

UbC

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pT3TS-spCas9 ABE-Umax-ex2-rest1

Plasmid

#222143

Purpose

Adenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Expression

Mutation

Promoter

T3

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pT3TS-spCas9 ABE-Umax-ex1-rest1

Plasmid

#222140

Purpose

Adenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Expression

Mutation

Promoter

T3

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pPB UbC Arrestin-uTEV1-V5; UAS-mCherry

Plasmid

#173122

Purpose

Expresses uTEV1 and reporter receiver construct in DIPG

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

Arrestin-uTEV1-V5; UAS-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

UbC

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pPB UbC Arrestin-TEVp(WT)-V5; UAS-mCherry

Plasmid

#173121

Purpose

Expresses TEVp(WT) and reporter receiver construct in DIPG

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

Arrestin-TEVp(WT)-V5; UAS-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

UbC

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp

Plasmid

#125228

Purpose

Expresses SPARK2 NanoLuc-βarrestin2-TEVp in mammalian cells

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-NanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp

Plasmid

#125235

Purpose

Expresses SPARK2 NanoLuc-βarrestin2-TEVp (no HA) in mammalian cells

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

NanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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JGE302_pAAV-hSyn-DIO-Myc-PYLcs-bArrestin2-YFP (p2)

Plasmid

#107247

Purpose

beta arrestin 2 fused to PYLcs for abscisic acid mediated recruitment of ABIcs labelled proteins.

Depositor

Bryan Roth

Article

Roth et al bioRxiv 251769

Insert

ARRB2

Use

AAV

Tags

MYC, PYLcs, and YFP

Expression

Mutation

Promoter

hSYN

Available since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pLX208-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp

Plasmid

#125231

Purpose

For generating SPARK2 NanoLuc-βarrestin2-TEVp lentivirus

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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E. coli ER1821ΔlacI

Bacterial Strain

#141407

Purpose

LacI-deficient E. coli host bacterium that produces β-galactosidase constitutively

Depositor

Antal Kiss

Article

Szentes et al Nucleic Acids Res. 2020 Mar 18;48(5):e28

Bacterial Resistance

None

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only
rEcoli XpS

Bacterial Strain

#192872

Purpose

MG1655-C321 -- All 321 UAG codons changed to UAA

Depositor

Jesse Rinehart

Article

Gassaway et al Nat Methods. 2022 Oct 24. pii: 10.1038/s

Bacterial Resistance

Bleocin (zeocin)

Species

Escherichia coli

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only
PCDNA3-b2AR-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

Plasmid

#113610

Purpose

PPI reporter

Depositor

Xiaokun Shu

Article

Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

Insert

beta 2 adrenergic receptor

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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PCDNA3-muOR-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

Plasmid

#113611

Purpose

PPI reporter

Depositor

Xiaokun Shu

Article

Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

Insert

mu opioid receptor

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pET E. coli expression vector with BioBrick polypromoter restriction sites (14-A)

Plasmid

#48307

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pET LIC cloning vector with BioBrick polycistronic restriction sites (9A)

Plasmid

#48283

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

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PCDNA3-PAR1-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

Plasmid

#113608

Purpose

PPI reporter

Depositor

Xiaokun Shu

Article

Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

Insert

PAR1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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PCDNA3-PAR2-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

Plasmid

#113609

Purpose

PPI reporter

Depositor

Xiaokun Shu

Article

Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

Insert

PAR2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSYN-bArrestin2-TevC-P2A-TdTomato-WPRE-bGHpA

Plasmid

#89873

Purpose

expresses iTango bArrestin2-TevC component and TdTomato bicistronically for AAV production

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

bArrestin2-TevC-P2A-TdTomato

Use

AAV

Tags

TdTomato

Expression

Mammalian

Mutation

R173H in TdTomato (Please see depositor comments …

Promoter

Human synapsin

Available since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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PCDNA3-S1P1-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

Plasmid

#113612

Purpose

PPI reporter

Depositor

Xiaokun Shu

Article

Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

Insert

S1P1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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