Skip to main content
Addgene
Showing: 101 - 120 of 595 results

  1. LMP AMPK alpha1 hp1

    Plasmid
    #89489
    Purpose
    shRNA against AMPK alpha1 in mouse/human to knock down protein expression
    Depositor
    Russell Jones
    Article
    Faubert et al Cell Metab. 2013 Jan 8;17(1):113-24. doi
    Insert
    hairpin against mouse/human AMPKalpha1 (PRKAA1, Prkaa1 Human, Mouse)
    Use
    Mouse Targeting and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 (The GFP-Puro is from a PGK promoter; the shRN…
    Available since
    May 19, 2017
    Availability
    Academic Institutions and Nonprofits only

  2. pcDNA5/FRT/TO-GAlpha15-RLuc8

    Plasmid
    #140984
    Purpose
    Encodes a G alpha subunit (GNA15) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlpha15-RLuc8 (GNA15 Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140984-plasmid-map-sequence-id-300775

    Enlarge

  4. pcDNA5/FRT/TO-GAlpha11-RLuc8

    Plasmid
    #140983
    Purpose
    Encodes a G alpha subunit (GNA11) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlpha11-RLuc8 (GNA11 Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140983-plasmid-map-sequence-id-300767

    Enlarge

  6. pcDNA5/FRT/TO-GAlpha13-RLuc8

    Plasmid
    #140986
    Purpose
    Encodes a G alpha subunit (GNA13) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlpha13-RLuc8 (GNA13 Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140986-plasmid-map-sequence-id-301587

    Enlarge

  8. pcDNA5/FRT/TO-GAlpha12-RLuc8

    Plasmid
    #140985
    Purpose
    Encodes a G alpha subunit (GNA12) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlpha12-RLuc8 (GNA12 Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140985-plasmid-map-sequence-id-300781

    Enlarge

  10. pDGB3_alpha1_35S-eGFP-T35S (GB4279)

    Plasmid
    #225434
    Purpose
    Transcriptional unit for constitutive expression of the enhanced GFP.
    Depositor
    Diego Orzaez
    Insert
    35S:eGFP:T35S
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225434-plasmid-map-sequence-id-447741

    Enlarge

  11. pDGB3 alpha1 P35S:BFP:T35S (GB3416)

    Plasmid
    #160637
    Purpose
    TU for the constitutive expression of Blue Fluorescent Protein
    Depositor
    Diego Orzaez
    Insert
    BFP
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    35S
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160637-plasmid-map-sequence-id-321961

    Enlarge

  12. pDNA3-Cerulean-FHA1-NES

    Plasmid
    #138202
    Purpose
    Encodes N-terminal (PAABD) fragment of FRET-based bimolecular kinase activity reporters (BimKARs); cytosol targeted.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    Cerulean-FHA1-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    138202-plasmid-map-sequence-id-307221

    Enlarge

  20. pDGB3_alpha1 P35S:SV40-AcrIIA4_CONb:Tnos (GB3344)

    Plasmid
    #160635
    Purpose
    TU for SV40-AcrIIA4 protein Codon optimized for N. benthamiana expression under the regulation of the 35S promoter and Tnos terminator
    Depositor
    Diego Orzaez
    Article
    Calvache et al Plant Biotechnol J. 2022 Feb;20(2):399-4
    Insert
    AcrIIA4
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    35S
    Available since
    Sept. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    160635-plasmid-map-sequence-id-427149

    Enlarge

Showing: 101 - 120 of 595 results