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Plasmid#190077Purposeexpress human ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance geneDepositorUseTagsExpressionMammalianMutationPromoternoneAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only
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H2A-mTurquoise-RHOA-G14V-deltaCAAX
Plasmid#176097PurposeConstitutively active, nucelar localized RhoADepositorInsertRHOA (RHOA Human)
UseTagsExpressionMammalianMutationG14VPromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
H2A-mTurquoise-RAC1-G12V-deltaCAAX
Plasmid#176095PurposeConstitutively active, nuclear localized Rac1DepositorInsertRAC1 (RAC1 Human)
UseTagsExpressionMammalianMutationG12VPromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBac[3xP3-gTc'v;Tc'rps3P-Tc'H2Av_EGFP]
Plasmid#86454PurposeNuclear reporter (histone-tagged EGFP) construct with the Tribolium specific ribosomal protein subunit 3 promoter (driving ubiquitous expression); (Transformation plasmid; Black eye marker)DepositorInsert3xP3-gTc'v;Tc'rps3P-Tc'H2Av_EGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSUPER retro puro macroH2A1 shRNA
Plasmid#30517DepositorInsertmH2A1 shRNA
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSUPER retro puro macroH2A2 shRNA
Plasmid#30518DepositorInsertmH2A2 shRNA
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(+)-HaloTag N58-(GGS)X6-hH2A
Plasmid#171785PurposeExpression of HaloTag N58-(GGS)X6-hH2A in mammalian cellsDepositorInsertHaloTag N58-(GGS)X6-hH2A
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(+)-hH2A-(GGS)X6-HaloTag C58
Plasmid#171431PurposeExpression of hH2A-(GGS)X6-HaloTag C58 in mammalian cellsDepositorInserthH2A-(GGS)X6-HaloTag C58
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBac[3xP3-gTc'v;Tc'puP-Tc'H2Av_EGFP]
Plasmid#86455PurposeNuclear reporter (histone-tagged EGFP) construct with Tribolium specific polyubiquitin promoter (driving ubiquitous expression); (Transformation plasmid; Black eye marker)DepositorInsert3xP3-gTc'v;Tc'puP-Tc'H2Av_EGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
AttB_ACE2[HA]_IRES_iRFP670-H2A-P2A-PuroR
Plasmid#190080Purposeexpress cytoplasmically HA-tagged human ACE2 with selectable markers iRFP670 and puromycin resistance geneDepositorInsertACE2 (ACE2 Human)
UseTagsHAExpressionMammalianMutationPromoternoneAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
H2A-mTurquoise-CDC42-G12V-deltaCAAX
Plasmid#176094PurposeConstitutively active, nuclear localized Cdc42DepositorInsertCDC42 (CDC42 Human)
UseTagsExpressionMammalianMutationG12VPromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-H2A-mScarlet-2A-AKT_KTR-mTq2-2A-ERK_KTR-mNG
Plasmid#129632PurposeVisualization of Akt and ERK activityDepositorInsertPB-H2A-mScarlet-2A-AKT_KTR-mTq2-2A-ERK_KTR-mNG
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-Flag-H2A K13-15-118-119R
Plasmid#63568PurposeExpression of human H2A (with multiple Lysine to Arginine point mutations) tagged with FlagDepositorInsertH2A (H2AC20 Human)
UseTagsFlag (MDYKDDDDKVPRIQ)ExpressionMammalianMutationLysine at 13, 15, 118, and 119 are now ArgininePromoterCMVAvailable sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
AttB_ACE2[R.ferrum]-2A-TMPRSS2_IRES_iRFP670-H2A-P2A-PuroR
Plasmid#190078Purposeexpress Rhinolophus ferrumequinum ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance geneDepositorInsertACE2 (TMPRSS2 Rhinolophus ferrumequinum)
UseTagsExpressionMammalianMutationPromoternoneAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
AttB_ACE2[dEcto]_IRES-mCherry-H2A-P2A-PuroR
Plasmid#171596PurposeEctodomain deleted negative control ACE2 from Bxb1 landing padDepositorInsertsUseSynthetic Biology; Promoterless bxb1 dna recombin…TagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBac[3xP3-gTc'v; Tc'aTub1P-loxP(mCherry)Sv40_Tc'H2Av_EGFP]
Plasmid#86456PurposeLox responder construct with floxed (same orientation; 2xloxP, 2xlox2272) mCherry (cy) body marker and H2Av::EGFP (nu) for Cre dependent cell clone marking; (Transformation plasmid; Black eye marker)DepositorInsert3xP3-gTc'v; Tc'aTub1P-loxP(mCherry)Sv40_Tc'H2Av_EGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pME-Luciferase-P2A-H2A-mCherry (JDW 926)
Plasmid#224488PurposeGateway compatible middle entry clone containing a Luciferase P2A H2A-mCherry dual reporter (Luciferase/nuclear red fluorescent reporter)DepositorInsertLuciferase-P2A-H2A-mCherry
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E-IRES-H2A-mCherry-SV40-pA (JDW 1256)
Plasmid#224532PurposeA Gateway compatible 3' entry clone containing an IRES H2A mCherry followed by a polyADepositorInsertIRES-H2A-mCherry-SV40pA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-Flag-H2A K5-9-118-119-125-127-129R
Plasmid#63565PurposeExpression of human H2A (with multiple Lysine to Arginine point mutations) tagged with FlagDepositorInsertH2A (H2AC20 Human)
UseTagsFlag (MDYKDDDDKVPRIQ)ExpressionMammalianMutationLysines at 5, 9, 118, 119, 125, 127, and 129 are…PromoterCMVAvailable sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
AttB_[Kozak-mut]ACE2_IRES-mCherry-H2A-P2A-PuroR
Plasmid#171595PurposeWeak human ACE2 abundance from Bxb1 landing padDepositorInsertsUseSynthetic Biology; Promoterless bxb1 dna recombin…TagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only