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pEntry_SARS-CoV-2-Membrane

Plasmid

#168880

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

SARS-CoV-2-Membrane (M )

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_SARS-CoV-1-Membrane

Plasmid

#168840

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

SARS-CoV-1-Membrane (M )

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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membrane-mCherry-SpVasa-C3

Plasmid

#185587

Purpose

to recruit Vasa-C3 to membrane

Depositor

Mamiko Yajima

Article

Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

Insert

Vasa-C3

Use

In vitro transcription (mrna synthesis)

Tags

mCherry and membrane targeting sequence from Neur…

Expression

Mutation

C3 mutant

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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membrane-mCherry-SpVasa

Plasmid

#185586

Purpose

to recruit Vasa to membrane

Depositor

Mamiko Yajima

Article

Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

Insert

Vasa

Use

In vitro transcription (mrna synthesis)

Tags

mCherry, S-Tag and membrane targeting sequence fr…

Expression

Mutation

Promoter

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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membrane-HsPLK1-dead

Plasmid

#185572

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

PLK1-dead (PLK1 Human)

Use

In vitro transcription (mrna synthesis)

Tags

membrane targeting sequence from Neuromodulin (ML…

Expression

Mutation

K82R kinase-dead mutant

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pSP64-membrane-mCherry-LOV

Plasmid

#135599

Purpose

Expression of membrane-mCherry-LOV mRNA for microinjection for use of optogenetics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

membrane-mCherry-LOV

Use

In vitro transcription (mrna synthesis)

Tags

LOV and mCherry

Expression

Mutation

Promoter

Unknown

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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Plasma membrane-targeted optoPLD

Plasmid

#140114

Purpose

Encodes CRY2-mCherry-PLD-P2A-CIBN-CAAX for mammalian expression

Depositor

Jeremy Baskin

Article

Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Phospholipase D

Use

Tags

CAAX, CIBN, CRY2, P2A, and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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Plasma membrane-targeted optodPLD

Plasmid

#140061

Purpose

Encodes CRY2-mCherry-dPLD-P2A-CIBN-CAAX for mammalian expression

Depositor

Jeremy Baskin

Article

Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Phospholipase D

Use

Tags

CAAX, CIBN, CRY2, P2A, and mCherry

Expression

Mammalian

Mutation

H170A (catalytic dead)

Promoter

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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cASIC1 concatemer - fully assembled

Plasmid

#233133

Purpose

To create cASIC1 channels of defined stoichiometry in mammalian cells

Depositor

David MacLean

Article

Purohit et al Biophys J. 2024 Oct 15;123(20):3507-3518

Insert

ASIC1 (ASIC1 Chicken)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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PhOTO-N (pMTB:memb-Cerulean-2A-H2B-Dendra2)

Plasmid

#92400

Purpose

Constitutive expression of nuclear green-to-red photoconvertible Dendra2 and the membrane blue fluorescent protein Cerulean.

Depositor

Periklis Pantazis

Article

Dempsey et al PLoS One. 2012;7(3):e32888. doi: 10.1371

Inserts

Dendra2
Cerulean

Use

Zebrafish expression

Tags

H2B tag and membrane tag

Expression

Mutation

Promoter

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pPW3756 : CMVd3-ERmembrane-HaloTag-KKMP

Plasmid

#185678

Purpose

Low-level transient expression of a single HaloTag protein with a C-terminal KKMP ER retention signal, targeted to the ER membrane.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERmembrane-HaloTag-KKMP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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PhOTO-M (pMTB:memb-Dendra2-2A-H2B-Cerulean)

Plasmid

#92401

Purpose

Constitutive expression of membrane green-to-red photoconvertible Dendra2 and the nuclear blue fluorescent protein Cerulean.

Depositor

Periklis Pantazis

Article

Dempsey et al PLoS One. 2012;7(3):e32888. doi: 10.1371

Inserts

Dendra2
Cerulean

Use

Zebrafish expression

Tags

H2B tag and membrane tag

Expression

Mutation

Promoter

bactin2

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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Nano-lantern-plasma membrane/pcDNA3

Plasmid

#51975

Purpose

mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to the plasma membrane

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern

Use

Tags

HIS and Lyn

Expression

Mammalian

Mutation

S257G in Rluc8

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pPW3757 : CMVd3-ERmembrane-2xHaloTag-KKMP

Plasmid

#185679

Purpose

Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERmembrane-2xHaloTag-KKMP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-Membrin-IRES-Puromycin

Plasmid

#134865

Purpose

Expresses EGFP-tagged Golgi apparatus marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Membrin (GOSR2 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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HA-MAO-N Mitochondrial Membrane-Targeting Cloning Vector

Plasmid

#139015

Purpose

To express a protein of interest fused to the N-terminus of HA-MAO for mitochondrial outer membrane targeting

Depositor

Ken-Ichi Takemaru

Type

Empty backbone

Use

Tags

HA-MAO

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMOS010: CheRiff-eGFP channelrhodopsin (plasma membrane)

Plasmid

#163044

Purpose

CheRiff-eGFP channelrhodopsin (plasma membrane)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

CheRiff

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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pPW3783 : CMVd3-ERmembrane-GST-2xHaloTag-KKMP

Plasmid

#185687

Purpose

Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane, fused with a GST for constitutive dimerization (making a HaloTag tetramer).

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERmembrane-GST-2xHaloTag-KKMP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pPW3781 : CMVd3-ERmembrane-GST-HaloTag-KKMP

Plasmid

#185686

Purpose

Low-level transient expression of a HaloTag protein targeted to the ER membrane, fused with a GST for constitutive dimerization.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERmembrane-GST-HaloTag-KKMP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5 FLAG membrane-bound lipin-1 (TMD)

Plasmid

#120277

Purpose

Expression of ER/Golgi-tethered lipin-1 in mammalian cells

Depositor

Sirio Dupont

Article

Romani et al Nat Cell Biol. 2019 Mar;21(3):338-347. d

Insert

Lipin-1 (Lpin1 Mouse)

Use

Tags

FLAG and in-frame fusion with the TMD Golgi-local…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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