Addgene

We narrowed to 439 results for: EB1

Showing: 101 - 120 of 439 results

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FUGW-eGFP-ZEB1 3'UTR

Plasmid

#81018

Purpose

FUGW lentiviral vector with constitutively expressed eGFP regulated by the human Zeb1 3'UTR. Brighter than destabilized d2GFP sensor reported in manuscript

Depositor

Jeffrey Rosen

Article

Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

Insert

Zeb1 3'UTR (UTS2R Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pET22b-mEB1-mCherry-6xHis

Plasmid

#130984

Purpose

Bacterial expression of mouse EB1-mCherry-6xHis for protein purification.

Depositor

Anne Straube

Article

Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

Insert

EB1-mCherry-6xHis (Mapre1 Mouse)

Use

Tags

mCherry-6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCI-neo-RL-ZEB1 200bmutx5

Plasmid

#35537

Depositor

Greg Goodall

Article

Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

Insert

3'UTR of ZEB1 (ZEB1 Human, Budding Yeast)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

5 mutations in miR200b seed regions of 3'UTR…

Promoter

Available since

May 10, 2012

Availability

Academic Institutions and Nonprofits only

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pEB1deltaC-2xEGFP shRNA resistant

Plasmid

#37925

Depositor

Torsten Wittmann

Article

Gierke et al Curr Biol. 2012 May 8;22(9):753-62. Epub

Insert

EB1 (MAPRE1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

deletion after aa248, TTCCTGT=>TCCCGGT

Promoter

CMV

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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human EB1 in PET28a for protein expression

Plasmid

#39297

Depositor

Tim Mitchison, Jennifer Tirnauer

Article

Tirnauer et al Mol Biol Cell. 2002 Oct . 13(10):3614-26

Insert

EB1 (MAPRE1 Human)

Use

Tags

6HIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
human EB1 fused to monomeric RFP

Plasmid

#39323

Depositor

Tim Mitchison, Jennifer Tirnauer

Inserts

EB1 (MAPRE1 Human)
mRFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pLJM1 Flag GFP 4E-BP1 Rheb15

Plasmid

#112761

Purpose

express GFP-4EBP1-Rheb15

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-GFP-4EBP1-Rheb15 (EIF4EBP1 Human)

Use

Lentiviral

Tags

Flag eGFP and Rheb15

Expression

Mutation

Promoter

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGZ136 (pRS406-pREB1-3FLAG-MNase-NLS)

Plasmid

#72273

Purpose

FLAG-Mnase expressed under the REB1 promoter

Depositor

Steven Henikoff

Article

Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.

Insert

3xFLAG tag and aa 83-231 of Mnase

Use

Tags

Expression

Mutation

Promoter

REB1

Available since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only
pLJM1 Flag GFP 4E-BP1 Rheb15 CAAX

Plasmid

#112762

Purpose

express GFP-4EBP1-Rheb15(CAAX mutation)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-GFP-4EBP1-Rheb15 (CAAX mutant) (EIF4EBP1 Human)

Use

Lentiviral

Tags

Rheb15(CAAX mutant)

Expression

Mutation

CAAX mutation to prevent lipid localization

Promoter

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-phsyn-FLEX-KASH-EGFP-U6-ShGmeb1

Plasmid

#129706

Purpose

Express cre-dependent KASH-EGFP and Gmeb1 shRNA

Depositor

Yi Zhang

Article

Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

Insert

KASH-EGFP

Use

AAV

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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human EB1-GFP mutagenized to remove terminal 7 aa (QEEQEEY) (JB162))

Plasmid

#39308

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

C-terminal 7 aa (QEEQEEY) removed

Promoter

CMV

Available since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only
human EB1-GFP mutagenized Y44 Q46 both converted to A (JB140)

Plasmid

#39301

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Y44A and Q46A

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
human EB1-GFP mutagenized K148 K150 K151 all converted to A (JB144)

Plasmid

#39305

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

K148A, K150A and K151A

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
human EB1-GFP mutagenized T249 D250 E251 all converted to A (JB146)

Plasmid

#39307

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

T249A, D250A and E251A

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
human EB1-GFP mutagenized D88 K89 D94 K95 all converted to A (JB141)

Plasmid

#39302

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

D88A, K89A, D94A and K95A

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
human EB1-GFP mutagenized Q109 W110 K112 K113 all converted to A (JB142)

Plasmid

#39303

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Q109A, W110A, K112A and K113A

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
human EB1-GFP mutagenized K122 D123 Y124 D125 all converted to A (JB143)

Plasmid

#39304

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

K122A, D123A, Y124A and D125A

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
human EB1-GFP mutagenized S16 R17 H18 D19 W23 N25 all converted to A (JB139)

Plasmid

#39300

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S16A, R17A, H18A, D19A, W23A and N25A

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
human EB1-GFP mutagenized E211 K212 E213 R214 D215 Y217 all converted to A (JB145)

Plasmid

#39306

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

E211A, K212A, E213A, R214A, D215A and Y217A

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pSpCas9_BB_2A-GFP_MAPRE1-gRNA#1

Plasmid

#107726

Purpose

Knock out of EB1 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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