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Showing: 11961 - 11980 of 24952 results

  2. Intron-Tagging-EGFP-Frame0-Parental-Minicircle

    Plasmid
    #216124
    Purpose
    Parental minicircle containing EGFP flanked by a splice acceptor and splice donor. Used with other intron tagging plasmids for placing the EGFP tag as a synthetic exon into frame 0 introns of target genes.
    Depositor
    Stefan Kubicek
    Article
    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d
    Insert
    sgRNA-SA-(GGGGS)x4-EGFP-(GGGGS)x4-SD
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216124-plasmid-map-sequence-id-428729

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  4. Intron-Tagging-mScarlet-Frame1-Parental-Minicircle

    Plasmid
    #216125
    Purpose
    Parental minicircle containing mScarlet-I flanked by a splice acceptor and splice donor. Used with other intron tagging plasmids for placing the mScarlet tag as a synthetic exon into frame 1 introns of target genes.
    Depositor
    Stefan Kubicek
    Article
    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d
    Insert
    sgRNA-SA-(GGGGS)x4-mScarlet-I-(GGGGS)x4-SD
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216125-plasmid-map-sequence-id-428733

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  6. Lamp1-YFP

    Plasmid
    #1816
    Purpose
    Mammalian expression of Lamp1 fused to YFP. Used for localization to the lysosome.
    Depositor
    Walther Mothes
    Article
    Sherer et al Traffic 2003 Nov;4(11):785-801.
    Insert
    lysosome associated membrane protein 1 (Lamp1 Rat)
    Use
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    D50E (not important for function of plasmid)
    Promoter
    Available since
    Feb. 16, 2006
    Availability
    Academic Institutions and Nonprofits only
    1816-plasmid-map-sequence-id-364671

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  10. TA1-DuET

    Plasmid
    #213382
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    TA1 (TAAR1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213382-plasmid-map-sequence-id-430737

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Showing: 11961 - 11980 of 24952 results