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Plasmid#155324PurposeBinary vector for expression of PULSE-controlled GBP-mCherryDepositorInsertLB_(etr)8-(C120)5-PhCMVmin-GBP-mCherry-T35S_RB
UseSynthetic Biology; Eukaryotic expressionTagsExpressionMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROF021
Plasmid#155326PurposeExpression of PULSE-controlled FLucDepositorInsert(etr)8-(C120)5-PhCMVmin-FLuc-TSV40
UseLuciferase and Synthetic Biology; Eukaryotic expr…TagsExpressionMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROF366
Plasmid#155332PurposeExpression of FLuc under the control of AtAP1 promoterDepositorInsertPAtAP1-FLuc-TSV40
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-MmCENP-O-GFP
Plasmid#205178PurposeExpresses mouse CENP-O C-term tagged with GFP under T7 promoter - designed for in vitro transcriptionDepositorInsertCENP-O (Cenpo Mouse)
UseIn vitro transcriptionTags5 glycines linker followed by GFPExpressionMammalianMutationWild type mouse CENP-OPromoterT7Available sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMVQA
Plasmid#197894PurposepCR®2.1-TOPO® backbone with 3' end of HIV-1(HXB2:9045 till poly-A (20))DepositorInsertHIV-1 3' end (HXB2: 9045 till poly-A(10)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
gZiPro W5F
Plasmid#198443PurposeActivity/inhibition assays, 19F-NMR assignmentDepositorInsertglycine linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationR95*A, W5FPromoterT7 promoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
gZiPro W89F
Plasmid#198447PurposeActivity/inhibition assays, 19F-NMR assignmentDepositorInsertglycine linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationR95*A, W89FPromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
gZiPro W83F
Plasmid#198446PurposeActivity/inhibition assays, 19F-NMR assignmentDepositorInsertglycine linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationR95*A, W83FPromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
gZiPro W69F
Plasmid#198445PurposeActivity/inhibition assays, 19F-NMR assignmentDepositorInsertglycine linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationR95*A, W69FPromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
eZiPro
Plasmid#198434PurposeActivity/inhibition assays, 19F-NMRDepositorInsertenzymatically cleavable linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
gZiPro W50F
Plasmid#198444PurposeActivity/inhibition assays, 19F-NMR assignmentDepositorInsertglycine linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationR95*A, W50FPromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN2-del130-133G-T97RH102G-dsRNAres_Y
Plasmid#148064PurposeInsect Expression of DmPAN2-del130-133G-T97RH102G-dsRNAresDepositorInsertDmPAN2-del130-133G-T97RH102G-dsRNAres (PAN2 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN2-del246-249-dsRNAres_Y
Plasmid#148065PurposeInsect Expression of DmPAN2-del246-249-dsRNAresDepositorInsertDmPAN2-del246-249-dsRNAres (PAN2 Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmPAN2-del130-133G-T97RH102G-dsRNAres_Y
Plasmid#148033PurposeInsect Expression of DmPAN2-del130-133G-T97RH102G-dsRNAresDepositorInsertDmPAN2-del130-133G-T97RH102G-dsRNAres (PAN2 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmPAN2-del246-249-dsRNAres_Y
Plasmid#148034PurposeInsect Expression of DmPAN2-del246-249-dsRNAresDepositorInsertDmPAN2-del246-249-dsRNAres (PAN2 Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmPAN2-del358-467-T97RH102G-dsRNAres_Y
Plasmid#148036PurposeInsect Expression of DmPAN2-del358-467-T97RH102G-dsRNAresDepositorInsertDmPAN2-del358-467-T97RH102G-dsRNAres (PAN2 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
p21StBD
Plasmid#190269PurposeConstitutively expresses a protein of interest fused to a GAL4 DNA-binding domain. Targeted integration to genomic locus YPRC-tau 3, Chromosome XVI.DepositorTypeEmpty backboneUseSynthetic Biology; Yeast genome-integrating vectorTagsGAL4-BDExpressionMutationPromoterTDH3Available sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHage2-EF1a-TERT K78E-IRES-Puro
Plasmid#182591Purposeexpresses TERT K78E in mammalian cellsDepositorInserthTERT (TERT Human)
UseLentiviralTagsExpressionMammalianMutationK78EPromoterEF-1aAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnCS_mEGFP-DSep2_Peanut-Strep
Plasmid#174496Purposebacterial co-expression of Drosophila Sep2 fused to monomeric EGFP and of Drosophila PeanutDepositorUseTagsStrep and mEGFPExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBtPIPLC_N168C His
Plasmid#173809PurposeExpression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cellsDepositorInsertBacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
UseTags6XHisExpressionBacterialMutationAsn168Cys for labelingPromoterT7Available sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only