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Plasmid#61270PurposeAlso referred to as pRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains a pBBR1 origin and chloramphenicol resistance.DepositorInsertCas9, tracrRNA, crRNA
UseCRISPRTags6xHisExpressionBacterialMutationPromoterpLtetO, native, native (respectively)Available sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSecEffector
Plasmid#172891PurposeContains TEVp display cassette, atc inducible (in any E. coli strain) BxbI and arabinose inducible TP901DepositorInsertsBxb1
TP901
TEV Display cassette
ProD promoter between anti aligned Bxb1 sites
UseTagsExpressionBacterialMutationPromoterProD, nTETO, and pBAD /AraCAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC-94N
Plasmid#53281PurposeContains crtI, crtY, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and produces beta-carotene in E. coli when complemented with a gene encoding geranylgeranyl diphosphate synthaseDepositorInsertcrtY, crtI, crtB
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCggRO-reporter
Plasmid#124582PurposeReporter plasmid with Constitutively expressed mCherry and CggR-regulated YFP (eCitrine)DepositorInsertCggR promoter element
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZR064_CROPseq-sgCD4-CRISPRi
Plasmid#180270PurposeLentiviral CROPseq vector expressing CD4 targeting sgRNA for CRISPRiDepositorInsertCD4 sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNeoXTR f0
Plasmid#69157Purposemouse targeting vectorDepositorInsertsDTA
NeoR
GFP
AmpR
UseMouse TargetingTagsExpressionMutationPromoterAmp promoter, PGK, and noneAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
rAAV-syn::FLEX-rev::PSAML141F:GlyR-IRES-GFP
Plasmid#32479DepositorInsertPSAML141F-GlyR-IRES-GFP
UseAAV and Cre/Lox; Adeno-associated virus, flex swiā¦TagsIRES-EGFPExpressionMutationPromoterSynapsinAvailable sinceJan. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
LR (bact: HyPer)
Plasmid#162069PurposeUsed for the diffuse visualization of H2O2 production in cells.DepositorInsertbeta actin 2 (actb2 Zebrafish)
UseGateway donor vectorTagsHyPerExpressionMutationPromoterT3Available sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUF-AsCpf1-pre-gRNA
Plasmid#137849PurposeAsCpf1 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.DepositorInsertAsCpf1 and pre-gRNA cassette
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTK333
Plasmid#59574PurposeSIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2 4xFF5DepositorInsertSGP(14) Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2 4xFF5
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mCherry2-G
Plasmid#164162PurposeExpresses mCherry2 under the control of CAG promoter, basic vector, multiclonig site at C terminalDepositorInsertmCherry2
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET15b LFn-Fla
Plasmid#84871PurposeBacterial expression of LFn-Fla fusionDepositorInsertsLFn
Fla
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTRE:shRNA A-pGK:rtTA-Cre
Plasmid#68471PurposeInducible lentivirus expressing an arbitrary shRNA and CreDepositorInsertsGFP-shRNA A-miR30
rtTA-2A-Cre
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterpGK and pTREAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
EmptyTranscriptionalReporter
Plasmid#62453PurposeMXS_chaining vector with 2 cassettesDepositorInsertsCMVmin::NLS-Citrine-bGHpA
CMV::NLS-Cherry-bGHpA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
Pprm1-luciferase; his-3
Plasmid#137728PurposeCommunication-activated luciferase in Neurospora crassaDepositorInsertsLuciferase
Pprm-1 Promoter
UseLuciferase; Neurospora crassa expressionTagsExpressionMutationCodon-optimized for N. crassaPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
cPE
Plasmid#183549PurposeExpresses compact Prime Editor in mammalian cellsDepositorInsertSpCas9 nickase (H840A), Reverse Transcriptase
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
Flag-nls-NgAgo-GK
Plasmid#86435PurposeExpresses a Flag tag on the N-terminalDepositorInsertNgAgo
UseTagsFlag tagExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
CAG:: ChR2HA-2a-hM4D
Plasmid#52520PurposeCo-expresses HA tagged channelrhodopsin and hM4DDepositorInsertChR2-2a-hM4D
UseTags2xHAExpressionMammalianMutationPromoterCAGAvailable sinceMay 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pStr-KDEL_EPV-SBP-DsRed2-ER-5
Plasmid#170676PurposeExpresses ER-targeted streptavidin-KDEL (hook) and SBP-DsRed2 targeted to the ER lumen with an N-terminal EPV tripeptide (cargo) for RUSH.DepositorInsertsEPV-SBP-DsRed2-ER-5
streptavidin-KDEL
UseTagsExpressionMammalianMutationPromoterIRES and T7Available sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMS29: pDonor(PmcCAST)
Plasmid#168162PurposePmcCAST donor harboring non-functional cargo.DepositorInsertsPmcCAST right end (RE)
PmcCAST left end (LE)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only