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Plasmid#62524PurposeN-terminal aa1-269 of Drosha is deletedDepositorInserthuman Drosha with aa 1-269 deleted (DROSHA Human)
UseTagsGFPExpressionMammalianMutationdeleted amino acids 1-269PromoterAvailable sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCfB3038(XII-1 MarkerFree)
Plasmid#73278PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-1 (Chr XII: 795787..796720)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB3040(XII-4 MarkerFree)
Plasmid#73280PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-4 (Chr XII:830227..831248)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKP4010
Plasmid#113336PurposeBeta-estradiol inducible 3X-FLAG Ty3 Integrase; Z4 promoter-3XFLAG Ty3 IN-CYC1 terminator; artificial transcription factor under ACT1 promoterDepositorInsertsArtificial Transcription Factor (McIsaac et al, 2013)
Ty3 Integrase
UseTags3X-FLAG/8X-Gly linkerExpressionYeastMutationPromoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
BII-ChBtW-dnTCF4-ires-GFP
Plasmid#133378PurposePiggybac vector for constitutive expressionDepositorInsertdnTCF4 (TCF4 Human)
UseTags2x FlagExpressionMammalianMutationdeltaN31PromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-DroshaS302D
Plasmid#62529PurposeSerine 302 of Drosha is mutated to aspartic acidDepositorInserthuman Drosha with serine 302 changed to aspartic acid (DROSHA Human)
UseTagsGFPExpressionMammalianMutationchanged serine 302 to aspartic acidPromoterAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-DroshaS300E
Plasmid#62528PurposeSerine 300 of Drosha is mutated to glutamic acidDepositorInserthuman Drosha with serine 300 changed to glutamic acid (DROSHA Human)
UseTagsGFPExpressionMammalianMutationchanged serine 300 to glutamic acidPromoterAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCfB3037(XI-5 MarkerFree)
Plasmid#73277PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-5 (Chr XI: 11779..118967)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK-FLAG-TRAF4(K190R)
Plasmid#58317PurposeFLAG-tagged mouse TRAF4 K190R mutantDepositorInsertTNF receptor associated factor 4 (Traf4 Mouse)
UseTagsFLAGExpressionMammalianMutationK 190 to RPromoterCMVAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO myc Separase delta 55/S1126A
Plasmid#59825PurposeAllows the integration of myc Separase delta 55/S1126A in the genome and Tet-inducible expression.DepositorInsertSeparase (ESPL1 Human)
UseTagsMycExpressionMammalianMutationdelta 55/S1126APromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pR2Q105y
Plasmid#101026Purposefluorescent, functional, glutamate receptor subunitDepositorInsertGlutamate receptor subunit (Gria2 Rat)
UseTagsfluorescent proteinExpressionMammalianMutationtagged with fluorescent proteinPromoterCMVAvailable sinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-TRAF4(K190R)
Plasmid#58319PurposeEGFP-tagged TRAF4 K190R mutantDepositorInsertTNF receptor associated factor 4 (Traf4 Mouse)
UseTagsGFPExpressionMammalianMutationK 190 to RPromoterCMVAvailable sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Fgß-Coh7-ddFLN4-ELP-HIS-ybbR
Plasmid#169135PurposeE. coli expression of Coh7 domain containing Fgß tag, ybbR tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.DepositorInsertCoh7
UseTags3xELP linker, 6x Histag, Fgβ peptide, ddFLN4 tag,…ExpressionBacterialMutationPromoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1(tandem wt-wt)
Plasmid#224757PurposeIn vitro transcription for Oocyte expression of mTREK-1(tandem wt-wt)DepositorInsertTandem TREK-1 (wt-wt) (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationPromoterT7Available sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACB108
Plasmid#222207PurposeFor recombinant expression of fghA (PP_1617 from Pseudomonas putida) with a C-terminal His tagDepositorInsertfghA
UseTags6x HisExpressionBacterialMutationPromoterT7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACB109
Plasmid#222208PurposeFor recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the K10N mutation and a C-terminal His tagDepositorInsertfghA (K10N)
UseTags6x HisExpressionBacterialMutationChanged Lys10 to AsnPromoterT7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACB110
Plasmid#222209PurposeFor recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the G258D mutation and a C-terminal His tagDepositorInsertfghA (G258D)
UseTags6x HisExpressionBacterialMutationChanged Gly258 to AspartatePromoterT7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW-HA-hRb-delta-CDK-puro
Plasmid#212673PurposeExpress tagged hRb delta-CDK phosphosite mutantDepositorInsertRb (RB1 Human)
UseLentiviralTagsHAExpressionMutationAll 15 CDK phospho-sites are mutated to alaninesPromoterTREAvailable sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMZ828
Plasmid#155322PurposeConstitutive expression of PhyB(1-650)-VP16 in plantsDepositorInsertPCaMV35S-PhyB(1-650)-VP16-NLS-Tnos
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNBA001
Plasmid#155323PurposeBinary vector for expression of PULSE-controlled EFR-GFPDepositorInsertLB_(etr)8-(C120)5-PhCMVmin-EFR-GFP-T35S_RB
UseSynthetic Biology; Eukaryotic expressionTagsExpressionMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only